TITLE

First detection of TEM-116 extended-spectrum ²-lactamase in a Providencia stuartii isolate from a Tunisian hospital

AUTHOR(S)
Lahlaoui, H; Dahmen, S; Moussa, MB; Omrane, B
PUB. DATE
July 2011
SOURCE
Indian Journal of Medical Microbiology;Jul2011, Vol. 29 Issue 3, p258
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes Materials and Methods: This strain was analysed by PCR and sequencing to identify the genes responsible for the β-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation. Results: This resistance was carried by a 60 kb plasmid that encoded a β-lactamase with a pI of 5.4. This β-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 β-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 β-lactamase. Conclusion: This study demonstrates the ?rst description of TEM-116 in the P. stuartii species in the world and the ?rst one in a Tunisian hospital.
ACCESSION #
65286920

 

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