Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy

Shubeita, G. T.; Sekatskii, S. K.; Dietler, G.; Letokhov, V. S.
April 2002
Applied Physics Letters;4/15/2002, Vol. 80 Issue 15, p2625
Academic Journal
We present fluorescence resonance energy transfer (FRET) images of donor dye molecule clusters recorded using a local fluorescence probe for scanning near-field optical microscopy (SNOM): standard apertured SNOM fiber tip coated with the 30–100-nm-thick polymer layer stained with the acceptor dye molecules. The tip works as a “self-sharpening pencil”: the apical layers of the FRET-active tip coating are mechanically worn out during scanning thus continuously exposing a fresh active apex to continue imaging. Only a few tens of acceptor molecules are used to form the optical images, and using such an approach spatial resolution better than the size of the aperture is achievable. © 2002 American Institute of Physics.


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