Identification of a Novel Splice Variant of C3G Which Shows Tissue-Specific Expression

Shivakrupa; Singh, Rita; Swarup, Ghanshyam
September 1999
DNA & Cell Biology;Sep99, Vol. 18 Issue 9, p701
Academic Journal
C3G is a guanine nucleotide-releasing protein that binds to the Src homology 3 (SH3) domain of the adapter protein Crk. In this study, we isolated cDNAs coding for rat C3G. Northern blot analysis of RNA from various rat tissues and cell lines showed a major transcript of about 7 kb which was present at the highest level in testis. A comparison of the amino acid sequence (derived from the cDNA sequence) of rat C3G with the human form showed 87.3% sequence identity. The principal difference was the presence of an additional 51 amino acids in the rat C3G sequence after the fifth PXXP motif. This difference may be attributable to alternative splicing of the primary transcript. This interpretation was supported by reverse transcription-polymerase chain reaction (RT-PCR) assays, which resulted in two products differing by 153 bp. The RT-PCR analysis of RNA from various rat tissues showed that the relative expression levels of the two splice forms were variable. The form of C3G with the insertion of 51 amino acids (named C3G-2) was present in rat testis at a high level and, to a lesser extent, in brain, but it was not seen (or was present at a very low level) in other rat tissues and certain rat and mouse cell lines. This expression pattern of the C3G-2 form was confirmed by Northern blotting using the insert region as a probe. The C3G-1 form, without the insertion of 51 amino acids, was present in almost all rat tissues except testis and in cell lines of rat, mouse, or human origin. Thus, in rat cells, we have identified a novel splice variant of C3G. The expression pattern indicates that the form of C3G described here is likely to serve a tissue- or cell-specific physiological function.


Related Articles

  • Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is up-regulated during spermiogenesis. Fujii, Takayuki; Tamura, Kazutoshi; Masai, Kumiko; Tanaka, Hiromitsu; Nishimune, Yoshitake; Nojima, Hiroshi // EMBO Reports;Apr2002, Vol. 3 Issue 4, p367 

    We report the isolation of 153 mouse genes whose expression is dramatically up-regulated during spermiogenesis. We used a novel variation of the subtractive hybridization technique called stepwise subtraction, wherein the subtraction process is systematically repeated in a stepwise manner. We...

  • PANGEA: pipeline for analysis of next generation amplicons. Giongo, Adriana; Crabb, David B.; Davis-Richardson, Austin G.; Chauliac, Diane; Mobberley, Jennifer M.; Gano, Kelsey A.; Mukherjee, Nabanita; Casella, George; Roesch, Luiz F. W.; Walts, Brandon; Riva, Alberto; King, Gary; Triplett, Eric W. // ISME Journal: Multidisciplinary Journal of Microbial Ecology;Jul2010, Vol. 4 Issue 7, p852 

    High-throughput DNA sequencing can identify organisms and describe population structures in many environmental and clinical samples. Current technologies generate millions of reads in a single run, requiring extensive computational strategies to organize, analyze and interpret those sequences. A...

  • Genomics: Analysis of complex traits in rats.  // Nature Methods;Aug2013, Vol. 10 Issue 8, p700 

    The article offers information on a research led by the Rat Genome Sequencing and Mapping Consortium which analyzed complex traits in rats by using genome sequencing and gene mapping techniques.

  • Molecular characterization of a voltage-gated potassium channel expressed in rat testis. Jacob, Asha // MHR: Basic Science of Reproductive Medicine;Apr2000, Vol. 6 Issue 4, p303 

    Examines the expression of calcium ion-activated potassium (K[sup +]) channels and delayed rectifier K[sup +] channels in rat testis. DNA sequences of K[sup +] channels; Presence of K[sup +] transcripts in cytoplasm of spermatocytes and spermatids; Amplification of K[sup +] channels.

  • Organization of the variable region of the immunoglobulin heavy-chain gene locus of the rat. Hendricks, Jacobus; Terpstra, Peter; Dammers, Peter M.; Somasundaram, Rajesh; Visser, Annie; Stoel, Maaike; Bos, Nicolaas A.; Kroese, Frans G. M. // Immunogenetics;Jul2010, Vol. 62 Issue 7, p479 

    We have mapped and annotated the variable region of the immunoglobulin heavy (IGH) gene locus of the Brown Norway (BN) rat (assembly V3.4; Rat Genomic Sequence Consortium). In addition to known variable region genes, we found 12 novel previously unidentified functional IGHV genes and 1 novel...

  • Baylor College of Medicine Garners Research Grants to Produce Rat Genome Sequence.  // Ascribe Newswire: Medicine;2/28/2001, p3 

    This article reports that Baylor College of Medicine in Houston, Texas announced two significant research grants that will support the Human Genome Sequencing Center's (HGSC) effort to determine the DNA sequence of laboratory rats. Baylor is the only medical school involved in the rat-sequencing...

  • Pseudogenes of rat VDAC1: 16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues. Ido, Yusuke; Yamamoto, Takenori; Yoshitomi, Tatsuki; Yamamoto, Atsushi; Obana, Eriko; Ohkura, Kazuto; Shinohara, Yasuo // Mammalian Genome;Apr2012, Vol. 23 Issue 3/4, p286 

    BLAST analysis of the rat genome revealed the presence of 16 pseudogenes of isoform 1 of the mitochondrial voltage-dependent anion channel (VDAC1). Based on their structural characterization, it was concluded that these pseudogenes were formed by integration of VDAC1 cDNA into the genome, and...

  • A Proposal to Sequence Genomes of Unique Interest for Research on Aging. De Magalhães, João Pedro; Sedivy, John M.; Finch, Caleb E.; Austad, Steven N.; Church, George M. // Journals of Gerontology Series A: Biological Sciences & Medical ;Jun2007, Vol. 62A Issue 6, p583 

    The article proposes the sequencing of genomes of unique interest for aging research. It emphasizes that genome sequencing will initiate a study on the mechanisms behind the evolution of longevity in mammals and in primates in particular. It cites that the proposed genomic resources will provide...

  • Critical Developmental Periods in the Pathogenesis of Hypertension. KUNEŠ, J.; KADLECOVÁ, M.; VANĚČKOVÁ, I.; ZICHA, J. // Physiological Research;2013 Supplement, Vol. 62, p1 

    Hypertension is one of the major risk factor of cardiovascular diseases, but after a century of clinical and basic research, the discrete etiology of this disease is still not fully understood. One reason is that blood pressure is a quantitative trait with multifactorial determination. Numerous...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics