TITLE

Expression and Molecular Cloning of Human Liver Leukotriene B4 omega-Hydroxylase (CYP4F2) Gene

AUTHOR(S)
Kikuta, Yasushi; Miyauchi, Yumiko; Kusunose, Emi; Kusunose, Masamichi
PUB. DATE
September 1999
SOURCE
DNA & Cell Biology;Sep99, Vol. 18 Issue 9, p723
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Human liver leukotriene B4 (LTB4) omega-hydroxylase (CYP4F2) plays an important role in the metabolic inactivation and degradation of LTB4, a potent mediator of inflammation. The regulatory mechanism for the transcription of CYP4F2 has not yet been clarified. Here, we report that CYP4F2 is constitutively expressed in a human hepatoma cell line, HepG2, and is not induced by clofibrate. We isolated the gene encoding CYP4F2 and determined its genomic organization and the functional activity of its promoters. The CYP4F2 gene contains at least 13 exons with its open reading frame being encoded from exon II to exon XIII. Exon I includes 49 bp of a 5' untranslated sequence. The structure of this gene is very similar to that of the CYP4F3 gene earlier reported by Kikuta et al. (DNA Cell Biol 1998;17:221-230). The 5' flanking sequence downstream from -165 of the CYP4F2 gene has 75% similarity to the corresponding region of the CYP4F3 gene. However, common putative regulating elements in the two human CYP4F genes were not detected except for the TATA box. The elements recognized by nuclear receptors were not observed within its 5' flanking region. Deletion of the 5' flanking regions containing putative regulating elements recognized by HNF-3beta, CDP CR, and p300 caused alterations in the transcriptional activity. The region from -83 to -67 was necessary for transcription, but the TATA sequence was not. Our results indicate that the human two CYP4F genes evolved by duplication and alterations of the transcription regulation region and the site of exon III.
ACCESSION #
6463059

 

Related Articles

  • Cloning and characterization of the thyroid iodide transporter. Dai, Ge; Levy, Orlie // Nature;2/1/1996, Vol. 379 Issue 6564, p458 

    Reports on the isolation of a complementary DNA clone that encodes the thyroid iodide transporter. Provision of a missing link in the thyroid hormone biosynthetic pathway; Nucleotide sequence of the sodium/iodide symport.

  • An Efficient Procedure for Cloning Hormone-Responsive Genes from a Specific Tissue. Korkmaz, Kemal S.; Korkmaz, Ceren G.; Ragnhildstveit, Erlend; Pretlow, Thomas G.; Saatcioglu, Fahri // DNA & Cell Biology;Aug2000, Vol. 19 Issue 8, p499 

    Nuclear receptors form a superfamily of ligand-activated transcription factors. In contrast to the significant advances made in recent years to dissect nuclear receptor structure and their corresponding function, progress has been rather slow in the identification of target genes for nuclear...

  • Molecular Cloning, Tissue Distribution, and Chromosomal Localization of MMEL2, a Gene Coding for a Novel Human Member of the Neutral Endopeptidase-24.11 Family. Bonvouloir, Nadia; Lemieux, Nicole; Crine, Philippe; Boileau, Guy; DesGroseillers, Luc // DNA & Cell Biology;Aug2001, Vol. 20 Issue 8, p493 

    Members of the neutral endopeptidase (NEP, also known as MME for membrane metallo-endopeptidase in the Human Gene Nomenclature database) family play significant roles in pain perception, arterial pressure regulation, phosphate metabolism, and homeostasis. In this paper, we report the cloning of...

  • Common Sense Rules Findings. Stringleman, Hugh // New Zealand Forest Industries;Sep2001, Vol. 32 Issue 9, p51 

    Focuses on the stress management in forest management industry in New Zealand. Modification of genetically combined trees; Discovery of gene cloning fragment on DNA in novel organism; Emphasis on biotechnology enhancement on competitiveness and sustainability of growth rates.

  • Sperm donors create super worker.  // Personnel Today;1/22/2002, p60 

    Reports the sperm donor campaign of a company to create its own staff through gene cloning scheme.

  • Expression of the Bacillus licheniformis PWD-1 keratinase gene in B. subtilis. Lin, X; Wong, S-L; Miller, E S; Shih, J C H // Journal of Industrial Microbiology & Biotechnology;Aug97, Vol. 19 Issue 2, p134 

    The kerA gene which encodes the enzyme keratinase was isolated from the feather-degrading bacterium Bacillus licheniformis PWD-1. The entire gene, including pre-, pro- and mature protein regions, was cloned with P[SUBker], its own promoter, P43, the vegetative growth promoter, or the combination...

  • Molecular cloning and characterization of a phytochelatin synthase gene, PvPCS1, from Pteris vittata L. Dong, Ruibin // Journal of Industrial Microbiology & Biotechnology;Aug2005, Vol. 32 Issue 8, p382 

    Presents a correction to the article "Molecular cloning and characterization of a phytochelatin synthase gene, PvPCS1, from Pteris vittata L.," by Ruibin Dong, which was published online on July 13, 2005.

  • Back from extinction. Pierce, Alan // Tech Directions;Jan2000, Vol. 59 Issue 6, p12 

    Reports on the possible cloning of the extinct prehistoric creature, the woolly mammoth. Existence of viable DNA for the creature; Cloning of the animal from dead cells; Possible use of Asian elephant as surrogate mother.

  • Molecular Cloning and Baculovirus Expression of the Rabbit Corneal Aldehyde Dehydrogenase (ALDH1A1) cDNA. Manzer, Rizwan; Qamar, Lubna; Estey, Tia; Pappa, Aglaia; Petersen, Dennis R.; Vasiliou, Vasilis // DNA & Cell Biology;May2003, Vol. 22 Issue 5, p329 

    Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics