TITLE

Sustained Mitogen-Activated Protein Kinase Activation Is Induced by Transforming erbB Receptor Complexes

AUTHOR(S)
Wu, Chuan-Jin; Qian, Xiaolan; O'Rourke, Donald M.
PUB. DATE
October 1999
SOURCE
DNA & Cell Biology;Oct99, Vol. 18 Issue 10, p731
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We used a genetic approach to characterize features of mitogen-activated protein kinase (MAPK) activation occurring as a consequence of expression of distinct erbB receptor combinations in transformed human cells. Kinase-deficient erbB proteins reduced epidermal growth factor (EGF)-induced tyrosine phosphorylation of endogenous Shc proteins and also reduced immediate and sustained EGF-induced ERK MAPK activities in human glioblastoma cells, although basal ERK MAPK activities were unaffected. Basal and EGF-induced JNK and p38 MAPK kinase activities were equivalent in parental cancer cells and EGFR-inhibited subclones. When ectopically overexpressed in murine fibroblasts and human glioblastoma cells, a constitutively activated human EGF receptor oncoprotein (Delta EGFR) induced EGF-independent elevation of basal ERK MAPK activity. Basal JNK MAPK kinase activity was also specifically induced by Delta EGFR, which correlated with increased phosphorylation of a 54-kDa JNK2 protein observed in Delta EGFR-containing cells. The JNK activities in response to DNA damage were comparably increased in cells containing wildtype EGFR or Delta EGFR. Consistent with the notion that transforming erbB complexes induce sustained and unregulated MAPK activities, coexpression of p185neu and EGFR proteins to levels sufficient to transform murine fibroblasts also resulted in prolonged EGF-induced ERK in vitro kinase activation. Transforming erbB complexes, including EGFR homodimers, Delta EGFR homodimers, and p185neu/EGFR heterodimers, appear to induce sustained, unattenuated activation of MAPK activities that may contribute to increased transformation and resistance to apoptosis in primary human glioblastoma cells.
ACCESSION #
6463058

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics