Characterization of Expression of the Gene for Human Pterin Carbinolamine Dehydratase/Dimerization Cofactor of HNF1

Lei, Xiang-Dong; Kaufman, Seymour
March 1999
DNA & Cell Biology;Mar1999, Vol. 18 Issue 3, p243
Academic Journal
Pterin carbinolamine dehydratase/dimerization cofactor of HNF1 (PCD/DCoH) is a dual-function protein. In the cytoplasm it acts as a dehydratase in the regeneration of tetrahydrobiopterin, the cofactor for aromatic amino acid hydroxylases. In the nucleus, it functions as a dimerization cofactor of HNF1 and increases the transcriptional activity of HNF1. To deepen our understanding of this protein, we characterized its expression in human tissues and cells. Human PCD/DCoH was present predominantly in liver and kidney, with significant amounts in testis and ovary, trace amounts in lung, and undetectable levels in whole brain, heart, and spleen. It was expressed in all of the cells that were examined. Importantly, it was also present in the nucleus of HeLa cells, which lack HNF1, and in the cytoplasm of fibroblasts that have little or no tetrahydrobiopterin. The expression of human PCD/DCoH in the liver and nonhepatic cells was compared at both the mRNA and protein levels. Although the mRNA level in liver was only fourfold higher than that in keratinocytes and fibroblasts, the hepatic PCD/DCoH protein level was 20-fold higher than that in normal human epidermal keratinocytes and dermal fibroblasts. Cloning of the 5' and 3' untranslated region (UTR) of human keratinocyte PCD/DCoH revealed that it has 53 bp more of GC-rich 5' untranslated sequence than the published liver PCD/DCoH. In vitro transcription and translation analysis showed that the longer 5' UTR resulted in about a 35% decrease in translation efficiency. These data show that human PCD/DCoH is not only present in cells where tetrahydrobiopterin is synthesized or HNF1 is present but is a widely distributed protein. Its differential expression in different tissues and cells is regulated not only at the transcriptional level but also at the translational level.


Related Articles

  • LMP2 expression and proteasome activity in NOD mice. Runnels, Herbert A.; Watkins, Wendy A.; Monaco, John J. // Nature Medicine;Oct2000, Vol. 6 Issue 10, p1064 

    Focuses on large multifunctional proteasome subunit (LMP) 2 expression and proteasome activity in non-obese diabetic (NOD) mice. Normal expression levels of LMP2; Western blot analysis; Homozygous deletion of genes.

  • In Situ Western Blot: A Novel Strategy to Study Gene Expression in Transgenic Plant Roots. Caiqing, Mo // Plant Molecular Biology Reporter;2000, Vol. 18 Issue 3, p289 

    Abstract. A novel strategy based on protein western blotting and "microcosm" principles has been developed to study gene expression in transgenic plant roots. Primary polyclonal antibodies, raised against streptavidin protein purified from Streptomyces avidinii, were used to localize...

  • Comparative analysis of the activation of MAP/ERK kinases in the CNS of animals with different learning abilities. L. Grinkevich; P. Lisachev; K. Baranova; O. Kharchenko // Neuroscience & Behavioral Physiology;Sep2007, Vol. 37 Issue 7, p715 

    Abstract  Western blot analysis was used to study the activation of MAP/ERK protein kinases responsible for controlling gene expression via phosphorylation of transcription factors CREB and ELK-1 in native common snails and animals with impaired abilities to form long-term types of...

  • Muscle-specific deletion of SOCS3 increases the early inflammatory response but does not affect regeneration after myotoxic injury. Swiderski, Kristy; Thakur, Savant S.; Naim, Timur; Trieu, Jennifer; Chee, Annabel; Stapleton, David I.; Koopman, René; Lynch, Gordon S. // Skeletal Muscle;10/24/2016, Vol. 6, p1 

    Background: Muscles of old animals are injured more easily and regenerate poorly, attributed in part to increased levels of circulating pro-inflammatory cytokines. The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling cascade is a key mediator of inflammatory...

  • biopterin.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p267 

    A definition of the term "biopterin," which is a by-product of pteridine that is important in metabolizing phenylalanine, is presented.

  • Enhanced Effects of 1,25(OH)2D3 Plus Genistein on Adipogenesis and Apoptosis in 3T3-L1 Adipocytes. Rayalam, Srujana; Della-Fera, Mary Anne; Ambati, Suresh; Jeong-Yeh Yang; Park, Hea Jin; Baile, Clifton A. // Obesity (19307381);Mar2008, Vol. 16 Issue 3, p539 

    The article focuses on a study which investigated the ability of 1,25(OH)2D3 and genistein (G), alone and in combination, to inhibit adipogenesis and induce apoptosis in 3T3-L1 adipocytes. Through western blotting, the expression of adipocyte-specific proteins and VDR was analyzed. The findings...

  • NOTCH-1 and NOTCH-4 are novel gene targets of PEA3 in breast cancer: novel therapeutic implications. Clementz, Anthony G.; Rogowski, Allison; Pandya, Kinnari; Miele, Lucio; Osipo, Clodia // Breast Cancer Research;2011, Vol. 13 Issue 3, p1 

    Introduction: Women with triple-negative breast cancer have the worst prognosis, frequently present with metastatic tumors and have few targeted therapy options. Notch-1 and Notch-4 are potent breast oncogenes that are overexpressed in triple-negative and other subtypes of breast cancer. PEA3,...

  • Photoperiodic Regulation of PER1 and PER2 Protein Expression in Rat Peripheral Tissues. Bendová, Z.; Sumová, A. // Physiological Research;2006, Vol. 55 Issue 6, p623 

    Circadian oscillations in biological variables in mammals are controlled by a central pacemaker in the suprachiasmatic nuclei (SCN) of the hypothalamus which coordinates circadian oscillators in peripheral tissues. The molecular clockwork responsible for this rhythmicity consists of several...

  • Transactivation of Protein Expression by Rice HSP101 in Planta and Using Hsp101 as a Selection Marker for Transformation. Ching-Chun Chang; Pin-Sheng Huang; Huei-Ru Lin; Chun-Hsien Lu // Plant & Cell Physiology;Aug2007, Vol. 48 Issue 8, p1098 

    Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics