A His2AvDGFP Fusion Gene Complements a Lethal His2AvD Mutant Allele and Provides an in Vivo Marker for Drosophila Chromosome Behavior

Clarkson, Michael; Saint, Robert
June 1999
DNA & Cell Biology;Jun99, Vol. 18 Issue 6, p457
Academic Journal
We have generated Drosophila melanogaster lines carrying a modified genomic fragment which encodes the D. melanogaster variant H2A.F/Z class histone, His2AvD, fused to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria. We show here that the fusion protein consists of functional GFP and functional histone His2AvD. The His2AvD portion of the fusion gene was shown to be functional by rescue of His2AvD mutant lethality. Fluorescence of the fusion protein in vivo was observed in embryonic cleavage stage interphase nuclei and on chromosomes as early as cycle 9, correlating with activation of transcription. Unlike transcription factors, the His2AvDGFP protein remained on transcriptionally inactive chromosomes throughout mitosis. Subsequently, fluorescence was observed in nuclei at all stages of embryonic and larval development and in adult somatic tissues, consistent with the distribution of His2AvD observed by immunohistochemical staining. This functional fusion histone acts as an excellent in vivo marker for chromosomes and chromosome behavior and, given the ability of the fusion gene to prevent null-mutant lethality, without disrupting normal cellular functions. The very high level of conservation of the H2A.F/Z family of variant histones suggests that the equivalent fusion protein construct should function equally well in a wide range of organisms.


Related Articles

  • LOOK INTO MY (GLOWING) EYES.  // Ecologist;Mar2001, Vol. 31 Issue 2, p8 

    Reports on the discovery of the glowing jellyfish gene. Purposes of the fluorescence gene; Information on a competition sponsored by the British Biotechnology and Biological Research Council in 2000 regarding the creation of a glowing tree.

  • Sea creatures.  // Boys' Life;Apr90, Vol. 80 Issue 4, p60 

    Reports on the medusa or jellyfish. Description; What they eat; Different kinds; The Portuguese man-of-war; First aid if you are stung by one.

  • Jellyfish Invasion. Puleo, Christine // Scholastic News -- Senior Edition;10/25/99, Vol. 68 Issue 7, p6 

    Provides information on jellyfish.

  • Jellyfish. Jaques, Susan // National Geographic World;Aug96, Issue 252, p7 

    Explains the nature of jellyfish. Physiology; Habitat; Food-hunting mechanism. INSET: Where danger lurks..

  • The world of jellyfish. Montgomery, Sy // Animals;Jul/Aug95, Vol. 128 Issue 4, p8 

    Features the jellyfish. Physical characteristics; Habitat; Adaptations; Classification; Locomotion; Food; Evolution; Behavior.

  • Stingers in the sea. Miller, Jeanne // World & I;Mar96, Vol. 11 Issue 3, p164 

    Discusses various aspects of jellyfish. Life cycle; Physical characteristics; Tentacles' sting. INSET: Avoiding a painful lesson, by J.M..

  • Just when you thought it was safe to go in the water.... Kageleiry, Jamie; Schultz, Christine // Yankee;Jul94, Vol. 58 Issue 7, p50 

    Recounts the discovery in 1865 of an Arctic giant jellyfish on the beach at Cape Ann, Massachusetts.

  • Space-age jewels. Dybas, Cheryl Lyn // National Wildlife (World Edition);Aug/Sep92, Vol. 30 Issue 5, p18 

    Examines the beneficial role jellyfish play in the oceans and in space experiments. Control of jellyfish prey; Size and territory; How jellyfish move; Food; Dangerous jellyfish; Details of National Aeronautics and Space Administration's (NASA) experiment; Government's Jellyfish Control Act,...

  • Crimson Tide. Snyder, Kari // Boating;Aug2003, Vol. 76 Issue 8, p25 

    Reports that scientists have discovered a variety of jellyfish, Tiburonia granrojo, in the Pacific Ocean.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics