TITLE

The Product of the Primary Response Gene BRF1 Inhibits the Interaction between 14-3-3 Proteins and cRaf-1 in the Yeast Trihybrid System

AUTHOR(S)
Bustin, Stephen A.; Mckay, Ian A.
PUB. DATE
August 1999
SOURCE
DNA & Cell Biology;Aug99, Vol. 18 Issue 8, p653
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The 14-3-3 proteins are small abundant cytosolic eukaryotic proteins that associate with and modulate the activity of numerous other proteins. The 14-3-3beta isoform has been shown to bind to the product of the protooncogene cRaf-1 and to facilitate its activation by Ras. Using the yeast two-hybrid system, we have demonstrated that 14-3-3beta and another isoform, 14-3-3tau, bind to the product of the primary response gene BRF1 and that the interaction between each isoform and BRF1 is significantly stronger than that with cRaf-1. We further demonstrated that the charge of residue 187 in 14-3-3beta regulates its affinity for both BRF1 and cRaf-1. The interaction of either isoform with BRF1 requires both proteins to be fully intact. When all three proteins are coexpressed in a yeast trihybrid system, BRF1 interferes significantly with the binding of 14-3-3 to full-length cRaf-1 as well as to its regulatory and kinase domains. Using quantitative reverse transcriptionpolymerase chain reaction, 14-3-3beta and BRF1 were found to be coexpressed in four different human tissues, suggesting a biologic role for their interaction in the regulation of cRaf-1-mediated signal transduction processes.
ACCESSION #
6462990

 

Related Articles

  • Grb2 binding to the different isoforms of Ret tyrosine kinase. Alberti, L; Borrello, M G; Ghizzoni, S; Torriti, F; Rizzetti, M G; Pierotti, M A // Oncogene;9/3/98, Vol. 17 Issue 9, p1079 

    The RET proto-oncogene encodes two isoforms of a receptor tyrosine kinase which plays a role in neural crest and kidney development. Ret ligands have been recently identified as the neuron survival factor GDNF (Glial-Derived Neurotrophic Factor) and Neurturin. Somatic rearrangements of RET,...

  • Signaling via Shc family adapter proteins. Ravichandran, Kodi S // Oncogene;10/1/2001 Review, Vol. 20 Issue 44, p6322 

    The adapter protein Shc was initially identified as an SH2 containing proto-oncogene involved in growth factor signaling. Since then a number of studies in multiple systems have implicated a role for Shc in signaling via many different types of receptors, such as growth factor receptors, antigen...

  • Regulation of the forkhead transcription factor FKHR, but not the PAX3-FKHR fusion protein, by the serine/threonine kinase Akt. del Peso, Luis; González, Víctor M; Hernández, Rubén; Barr, Frederic G; Núñez, Gabriel // Oncogene;12/2/99, Vol. 18 Issue 51, p7328 

    AKT:, a proto-oncogene that encodes a cytosolic serine/threonine kinase, can phosphorylate and modulate the activity of several proteins involved in cellular metabolism and survival. Recently, two mammalian highly related forkhead transcription factors FKHRL1 and AFX and their nematode homologue...

  • Stability of p21Waf1/Cip1 CDK inhibitor protein is responsive to RhoA-mediated regulation of the actin cytoskeleton. Coleman, M. L.; Densham, R. M.; Croft, D. R.; Olson, M. F. // Oncogene;5/4/2006, Vol. 25 Issue 19, p2708 

    The proto-oncogene Ras GTPase stimulates transcription of p21Waf1/Cip1 (p21), which is repressed by the RhoA GTPase. We previously showed that Ras also elevates p21 protein levels by reducing its proteasome-mediated degradation. Therefore, we investigated whether RhoA also influenced p21 protein...

  • Comprehensive Gene-Based Association Study of a Chromosome 20 Linked Region Implicates Novel Risk Loci for Depressive Symptoms in Psychotic Illness. Bigdeli, T. Bernard; Maher, Brion S.; Zhao, Zhongming; van den Oord, Edwin J. C. G.; Thiselton, Dawn L.; Sun, Jingchun; Webb, Bradley T.; Amdur, Richard L.; Wormley, Brandon; O'Neill, Francis A.; Walsh, Dermot; Riley, Brien P.; Kendler, Kenneth S.; Fanous, Ayman H. // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    Background: Prior genomewide scans of schizophrenia support evidence of linkage to regions of chromosome 20. However, association analyses have yet to provide support for any etiologically relevant variants. Methods: We analyzed 2988 LD-tagging single nucleotide polymorphisms (SNPs) in 327 genes...

  • Breast cancer and a proto-oncogene. Barnes, Diana M. // BMJ: British Medical Journal (International Edition);10/28/89, Vol. 299 Issue 6707, p1061 

    Reports the significance of proto-oncogene in managing patients with breast cancer in Great Britain. Determination of the proportion of patients with c-erbB-2 protein amplification through DNA analysis or immunohistochemistry; Relationship between c-erbB-2 and poor prognosis; Differentiation of...

  • Wnt Signaling in Development, Disease and Translational Medicine. Coombs, Gary S.; Covey, Tracy M.; Virshup, David M. // Current Drug Targets;Jul2008, Vol. 9 Issue 7, p513 

    Wnt signaling regulates a multitude of critical processes in development and tissue homeostasis. The wingless (wg) gene product was first identified in Drosophila in 1973. Subsequently, the proto-oncogene INT-1 was identified in mice in 1984 when its activation by mouse mammary tumor virus'...

  • Computer-Aided Drug Design for Cancer-Causing H-Ras p21 Mutant Protein. Jayakanthan, Mannu; Wadhwa, Gulshan; Mohan, Thangavel Madhan; Arul, Loganathan; Balasubramanian, Ponnusamy; Sundar, Durai // Letters in Drug Design & Discovery;Jan2009, Vol. 6 Issue 1, p14 

    GTP-bound mutant form H-Ras (Harvey-Ras) proteins are found in 30% of human tumors. Activation of H-Ras is due to point mutation at positions 12, 13, 59 and/or 61 codon. Mutant form of H-Ras proteins is continuously involved in signal transduction for cell growth and proliferation through...

  • The Wnt antagonist Dickkopf-1 is regulated by Bmp signaling and c-Jun and modulates programmed cell death. Grotewold, Lars; Rüther, Ulrich // EMBO Journal;3/1/2002, Vol. 21 Issue 5, p966 

    Dickkopf-1 (Dkk-1) has been shown to be a potent inhibitor of Wnt/β-catenin signaling in a variety of assays and organisms. In this study, we show that expression of Dkk-1 overlaps significantly with the sites of programmed cell death in normal as well as mutant vertebrate limb development,...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics