Identification and Characterization of a Transcript for a Novel Rac GTPase-Activating Protein in Terminally Differentiating 3T3-L1 Adipocytes

Wooltorton, Eric J.; Haliotis, Tina; Mueller, Christopher R.
April 1999
DNA & Cell Biology;Apr99, Vol. 18 Issue 4, p265
Academic Journal
Using differential display, we sought to identify novel genes expressed in the early stages of 3T3-L1 adipocyte differentiation. A gene which we have named "band25" was identified, and a full-length cDNA sequence was assembled. Sequence analysis revealed that the 2842-bp cDNA encodes a putative 628-amino acid protein product, which is a member of the GTPase-activating protein (GAP) family. This gene may be the murine homolog of the human M gcRacGAP protein, which was identified in male germ cells. Other closely related proteins include the Drosophila protein Rotund, several chimerins, and the human breakpoint cluster region (Bcr) protein. These GAP proteins all specifically inactivate Rac, a member of the Ras-like family of proteins. A consensus sequence for a diacyl glycerol/phorbol ester-binding domain was also found in the Band25 sequence. The expression of band25 mRNA is regulated during the differentiation of both adipocytes and myoblasts. Its mRNA was shown to be expressed at a low level in confluent 3T3-L1 preadipocytes and in differentiated 3T3-L1 adipocytes. Expression of band25 was increased 15.5 fold by 24 h after the induction of differentiation, when 3T3-L1 cells undergo several rounds of postconfluent cell division. Expression was also high in growing 3T3-L1 and C2C12 cells but decreased progressively as C2C12 cells underwent differentiation. These observations suggest that the expression of band25 is growth regulated and that the protein could play a role in the regulation of growth-related processes.


Related Articles

  • Rho3p Regulates Cell Separation by Modulating Exocyst Function in Schizosaccharomyces pombe. Hongyan Wang; Xie Tang; Balasubramanian, Mohan K. // Genetics;Aug2003, Vol. 164 Issue 4, p1323 

    Reports the isolation of rho3[sup +], encoding a Rho family GTPase, as a high-copy suppressor of an exocyst mutant sec8-1 in Schizosaccharomyces pombe. Regulation of cell separation by Rho3p; Aspects of cytokinesis; Suppression of the temperature-sensitive growth phenotype observed in cells...

  • Rab18 Dynamics in Adipocytes in Relation to Lipogenesis, Lipolysis and Obesity. Pulido, Marina R.; Diaz-Ruiz, Alberto; Jiménez-Gómez, Yolanda; Garcia-Navarro, Socorro; Gracia-Navarro, Francisco; Tinahones, Francisco; López-Miranda, José; Frühbeck, Gema; Vázquez-Martínez, Rafael; Malagón, Maria M. // PLoS ONE;2011, Vol. 6 Issue 7, p1 

    Lipid droplets (LDs) are organelles that coordinate lipid storage and mobilization, both processes being especially important in cells specialized in managing fat, the adipocytes. Proteomic analyses of LDs have consistently identified the small GTPase Rab18 as a component of the LD coat....

  • Arabidopsis SHORT INTEGUMENTS 2 Is a Mitochondrial DAR GTPase. Hill, Theresa A.; Broadhvest, Jean; Kuzoff, Robert K.; Gasser, Charles S. // Genetics;Oct2006, Vol. 174 Issue 2, p707 

    The Arabidopsis short integuments 2-1 (sin2-1) mutant produces ovules with short integuments due to early cessation of cell division in these structures. SIN2 was isolated and encodes a putative GTPase sharing features found in the novel DAR GTPase family. DAR proteins share a signature DAR...

  • Tetramerization of ZapA is required for FtsZ bundling. PACHECO-GÓMEZ, Raúl; Xi CHENG; HICKS, Matthew R.; SMITH, Corinne J. I.; ROPER, David I.; ADDINALL, Stephen; RODGER, Alison; DAFFORN, Timothy R. // Biochemical Journal;2/1/2013, Vol. 449 Issue 3, p795 

    Prokaryotic cell division is a highly orchestrated process requiring the formation of a wide range of biomolecular complexes, perhaps the most important of these involving the prokaryotic tubulin homologue FtsZ, a fibre-forming GTPase. FtsZ assembles into a ring (the Z-ring) on the inner surface...

  • Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells. Ciciarello, Marilena; Roscioli, Emanuele; Fiore, Barbara Di; Francesco, Laura Di; Sobrero, Fabrizia; Bernard, Delphine; Mangiacasale, Rosamaria; Harel, Amnon; Schininà, Maria Eugenia; Lavia, Patrizia // Chromosoma;Dec2010, Vol. 119 Issue 6, p651 

    The GTPase Ran regulates nucleocytoplasmic transport in interphase and spindle organisation in mitosis via effectors of the importin beta superfamily. Ran-binding protein 1 (RanBP1) regulates guanine nucleotide turnover on Ran, as well as its interactions with effectors. Unlike other Ran network...

  • Analysis of septins across kingdoms reveals orthology and new motifs. Pan, Fangfang; Malmberg, Russell L.; Momany, Michelle // BMC Evolutionary Biology;2007, Vol. 7, p103 

    Background: Septins are cytoskeletal GTPase proteins first discovered in the fungus Saccharomyces cerevisiae where they organize the septum and link nuclear division with cell division. More recently septins have been found in animals where they are important in processes ranging from actin and...

  • Essential Role of the Small GTPase Ran in Postnatal Pancreatic Islet Development. Xia, Fang; Dohi, Takehiko; Martin, Nina M.; Raskett, Christopher M.; Liu, Qin; Altieri, Dario C. // PLoS ONE;2011, Vol. 6 Issue 11, p1 

    The small GTPase Ran orchestrates pleiotropic cellular responses of nucleo-cytoplasmic shuttling, mitosis and subcellular trafficking, but whether deregulation of these pathways contributes to disease pathogenesis has remained elusive. Here, we generated transgenic mice expressing wild type (WT)...

  • Mutations in the bacterial cell division protein FtsZ highlight the role of GTP binding and longitudinal subunit interactions in assembly and function. Arjes, Heidi A.; Lai, Bradley; Emelue, Ezinwanne; Steinbach, Adriana; Levin, Petra Anne // BMC Microbiology;10/13/2015, Vol. 15, p1 

    Background: Assembly of the tubulin-like GTPase, FtsZ, at the future division site initiates the process of bacterial cytokinesis. The FtsZ ring serves as a platform for assembly of the division machinery and constricts at the leading edge of the invaginating septum during cytokinesis. In vitro,...

  • Novel functions of endocytic player clathrin in mitosis. Fu, Wenxiang; Jiang, Qing; Zhang, Chuanmao // Cell Research;Dec2011, Vol. 21 Issue 12, p1655 

    Clathrin has been widely recognized as a pivotal player in endocytosis, in which several adaptors and accessory proteins are involved. Recent studies suggested that clathrin is also essential for cell division. Here this review mainly focuses on the clathrin-dependent mechanisms involved in...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics