TITLE

Effects of sera taken from women with recurrent spontaneous abortion on sperm motility and apoptosis

AUTHOR(S)
Talaei-khozani, Tahereh; Borzoei, Zahra; Bahmanpour, Soghra; Zolghadr, Jaleh; Dehbashi, Sedigheh; Zareh, Hamid Reza
PUB. DATE
April 2011
SOURCE
Iranian Journal of Reproductive Medicine;Spring2011, Vol. 9 Issue 2, p125
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities. Objective: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility. Materials and Methods: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test. Results: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant. Conclusion: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.
ACCESSION #
64445398

 

Related Articles

  • Exposure to CB-153 and p,p′-DDE and male reproductive function. A. Rignell-Hydbom; L. Rylander; A. Giwercman; B.A.G. Jönsson; P. Nilsson-Ehle; L. Hagmar // Human Reproduction;Sep2004, Vol. 19 Issue 9, p2066 

    BACKGROUND: During the last decades, there has been concern that exposure to endocrine disruptors, such as persistent organochlorine pollutants (POPs), may contribute to an impairment of male reproductive function. To investigate whether exposure to...

  • Effect of Bovine Serum Albumin on Motility, Plasmalemma, Viability and Chromatin Integrity of Buffalo Bull Spermatozoa. Akhter, Shamim; Allah Rakha, Bushra; Iqbal, Razia; Sajjad Ansari, Muhammad // Pakistan Journal of Zoology;2014, Vol. 46 Issue 1, p115 

    The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in extender on post-thaw quality of buffalo spermatozoa. Semen was collected from three buffalo bulls with artificial vagina (42°C) for three weeks (replicates). Qualifying semen...

  • Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status. Domosławska, Anna; Zduńczyk, Sławomir; Janowski, Tomasz // Journal of Veterinary Research (2450-7393);Jun2019, Vol. 63 Issue 2, p293 

    Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. The dogs (a Golden Retriever, an English Cocker Spaniel,...

  • Effects of Tumour Necrosis Factor-α on Human Sperm Motility and Apoptosis. Anna Perdichizzi; Ferdinando Nicoletti; Sandro La Vignera; Nunziata Barone; Rosario D’Agata; Enzo Vicari // Journal of Clinical Immunology;Mar2007, Vol. 27 Issue 2, p152 

    The aim of this study was to evaluate the effects of tumour necrosis factor-? (TNF-?) on sperm motility, mitochondrial membrane potential (??), phosphatidylserine (PS) externalization, sperm chromatin packaging quality, and DNA fragmentation. Motile spermatozoa, obtained from 10 normozoospermic...

  • Older sperm develop defects similar to older eggs. Schieszer, John // Urology Times;Apr2003, Vol. 31 Issue 4, p18 

    Reports on a study which shows that men over age 35 have less sperm motility, apoptosis and more DNA defects than younger men. Research conducted at the University of Washington in Seattle; Effect of age and inflammation on sperm DNA damage and apoptosis; Warning that couples should try to have...

  • Protective role of glutathione in buck semen cryopreservation. Noei Razliqi, R.; Zhandi, M.; Shakeri, M.; Towhidi, A.; Sharafi, M.; Emamverdi, M.; Khodaei Motlagh, M. // Iranian Journal of Veterinary Research;2015, Vol. 16 Issue 3, p298 

    The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total...

  • Candida albicans experimental infection: effects on human sperm motility, mitochondrial membrane potential and apoptosis. Burrello, Nunziatina; Salmeri, Mario; Perdichizzi, Anna; Bellanca, Salvatore; Pettinato, Giuseppe; D'Agata, Rosario; Vicari, Enzo; Calogero, Aldo E. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Apr2009, Vol. 18 Issue 4, p496 

    Studies suggest Candida albicans infection has a negative effect on sperm function, including fertilizing ability. Assisted reproduction treatment using spermatozoa from a patient with unrecognized C. albicans infection did not result in fertilization. Preliminary evidence suggested an effect on...

  • Cigarette smoke extract immobilizes human spermatozoa and induces sperm apoptosis. Calogero, Aldo; Polosa, Riccardo; Perdichizzi, Anna; Guarino, Francesca; La Vignera, Sandro; Scarfia, Alessia; Fratantonio, Enza; Condorelli, Rosita; Bonanno, Oriana; Barone, Nunziata; Burrello, Nunziatina; D'Agata, Rosario; Vicari, Enzo // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2009, Vol. 19 Issue 4, p564 

    Cigarette smoking by the male partner adversely affects assisted reproductive techniques, suggesting that it may damage sperm chromatin/DNA and consequently embryo development. The effects of graded concentrations of research cigarettes smoke extract (CSE) on motility, mitochondrial membrane...

  • Effects of Partial Defect in Kit Gene Expression.  // Fertility Weekly;1/20/2003, p15 

    Presents a study in France on the effects of partial defect in Kit gene expression in mice. Impairment of sperm production; Increase of germ cell apoptosis; Impairment of the transition of type A spermatogonia to type B spermatogonia; Alteration in sperm motility.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics