TITLE

Distant interactions between enhancers and promoters in Drosophila melanogaster are mediated by transgene-flanking Su(Hw) insulators

AUTHOR(S)
Davydova, A.; Erokhin, M.; Georgiev, P.; Chetverina, D.
PUB. DATE
August 2011
SOURCE
Russian Journal of Genetics;Aug2011, Vol. 47 Issue 8, p917
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Insulators are DNA elements modulating gene activation by enhancers. Interaction between insulators can result in either isolation, or activation of promoter by the enhancer. In the present study, it is demonstrated that in transgenic lines, the yellow enhancers are unable to activate promoter of the target gene through the mini-white gene. The mini-white promoter, which functions as an insulator, can block the enhancer activity. In case that the genes and regulatory elements in the construct are flanked by Su(Hw) insulators from retrotransposon MDG4, interaction between enhancers and the yellow promoter is restored. The data obtained are congruent with the model suggesting that promoters can function as insulators, and that interaction between the insulators facilitates the interaction between enhancers and promoters within transcriptional domain.
ACCESSION #
64418079

 

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