Distant interactions between enhancers and promoters in Drosophila melanogaster are mediated by transgene-flanking Su(Hw) insulators

Davydova, A.; Erokhin, M.; Georgiev, P.; Chetverina, D.
August 2011
Russian Journal of Genetics;Aug2011, Vol. 47 Issue 8, p917
Academic Journal
Insulators are DNA elements modulating gene activation by enhancers. Interaction between insulators can result in either isolation, or activation of promoter by the enhancer. In the present study, it is demonstrated that in transgenic lines, the yellow enhancers are unable to activate promoter of the target gene through the mini-white gene. The mini-white promoter, which functions as an insulator, can block the enhancer activity. In case that the genes and regulatory elements in the construct are flanked by Su(Hw) insulators from retrotransposon MDG4, interaction between enhancers and the yellow promoter is restored. The data obtained are congruent with the model suggesting that promoters can function as insulators, and that interaction between the insulators facilitates the interaction between enhancers and promoters within transcriptional domain.


Related Articles

  • Genome-guided transcript assembly by integrative analysis of RNA sequence data. Boley, Nathan; Stoiber, Marcus H; Booth, Benjamin W; Wan, Kenneth H; Hoskins, Roger A; Bickel, Peter J; Celniker, Susan E; Brown, James B // Nature Biotechnology;Apr2014, Vol. 32 Issue 4, p341 

    The identification of full length transcripts entirely from short-read RNA sequencing data (RNA-seq) remains a challenge in the annotation of genomes. Here we describe an automated pipeline for genome annotation that integrates RNA-seq and gene-boundary data sets, which we call Generalized RNA...

  • Endeavours of RuBisCO Small Subunit Promoter as a Tool of Green Tissue Specific Expression. BAKHSH, ALLAH; HUSNAIN, TAYYAB // Czech Journal of Genetics & Plant Breeding;2012, Vol. 48 Issue 1, p1 

    Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The successful application of transgenic technology has been further strengthened by the availability...

  • Functional interactions between promoters of neighboring yellow and CG3777 genes in Drosophila melanogaster. Leman, D.; Parshikov, A.; Georgiev, P.; Maksimenko, O. // Russian Journal of Genetics;Dec2012, Vol. 48 Issue 12, p1163 

    It was shown by us previously that the transcription of the yellow gene can be affected by the promoter of the neighboring gene CG3777, which has a similar expression profile. In the present work, we continued studying the functional interactions between the promoters of the yellow and CG3777...

  • SV40 transcription terminators stabilize the activity of regulatory elements in Drosophila melanogaster. Chetverina, D.; Elizar'ev, P.; Georgiev, P.; Erokhin, M. // Doklady Biochemistry & Biophysics;Jul2015, Vol. 463 Issue 1, p251 

    To ensure stable operation of transgenic systems and maintain reporter gene expression at a certain level, it is necessary to search for the conditions that protect the activity of regulatory elements. In this study, we have shown that the SV40 transcription terminators flanking the transgene...

  • Organization of the Drosophila melanogaster SF1 insulator and its role in transcription regulation in transgenic lines. Leman, D.; Parshikov, A.; Georgiev, P.; Maksimenko, O. // Russian Journal of Genetics;Apr2014, Vol. 50 Issue 4, p341 

    The SF1 insulator was found to contain a polyadenylation signal, which corresponded to the functional polyadenylation signal in embryonic S2 cells and the transgenic lines of Drosophila and bi-directional promoter that functioned in S2 cells. The studies performed did not confirm the ability of...

  • Molecular variability in geographically distant populations of Drosophila melanogaster at the Lim3 gene regulating nervous system development. Veselkina, E.; Rybina, O.; Symonenko, A.; Alatortsev, V.; Roshchina, N.; Pasyukova, E. // Russian Journal of Genetics;Jun2014, Vol. 50 Issue 6, p549 

    In this study, we compare the variability of the regulatory region of the Lim3 gene, which plays a key role in the development of the nervous system, in two populations of Drosophila melanogaster inhabiting the cities of Aleksandrov (Russia) and Raleigh (United States). The two population areas...

  • MITIE: Simultaneous RNA-Seq-based transcript identification and quantification in multiple samples. Behr, Jonas; Kahles, André; Zhong, Yi; Sreedharan, Vipin T.; Drewe, Philipp; Rätsch, Gunnar // Bioinformatics;Oct2013, Vol. 29 Issue 20, p2529 

    Motivation: High-throughput sequencing of mRNA (RNA-Seq) has led to tremendous improvements in the detection of expressed genes and reconstruction of RNA transcripts. However, the extensive dynamic range of gene expression, technical limitations and biases, as well as the observed complexity of...

  • Complex Organization of Promoter and Enhancer Elements Regulate the Tissue- and Developmental.... Keplinger, Brian L.; Xinmei Guo // Genetics;Feb2001, Vol. 157 Issue 2, p699 

    Examines the organization of promoter and enhancer elements regulating glucose dehydrogenase (GLD) gene expression in Drosophila melanogaster. Related studies on the GLD gene; Description of element reproductive and nonreproductive tissue expression; Model of GLD transcriptional regulation;...

  • Search for new regulatory elements of theBithoraxcomplex inDrosophila melanogaster. Melnik, E.S.; Kyrchanova, O.V.; Gruzdeva, N.M.; Georgiev, P.G. // Russian Journal of Genetics;Dec2004, Vol. 40 Issue 12, p1331 

    TheUltrabithorax ( Ubx ),Abdominal-A ( Abd-A ), andAbdominal-B ( Abd-B )homeotic genes of theBithoraxcomplex are responsible for the development of body segments inDrosophila melanogaster. Their regulatory region of approximately 300 kb harbors a series of specific enhancers, each regulating...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics