Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples

Prasad, Dinesh; Shankaracharya; Vidyarthi, Ambarish Sharan
September 2011
World Journal of Microbiology & Biotechnology;Sep2011, Vol. 27 Issue 9, p2227
Academic Journal
A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel with the conventional culture method. The sensitivity and specificity of colorimetric assay was 89.15 and 99.04% respectively with reference to conventional culture method. The total time required to detect the Salmonella spp. present in food samples by the developed method is less than 8 h, including 6 h incubation. It was observed that the colorimetric assay was 10 times more sensitive than gel-based detection with the same concentration of DNA used for analysis.


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