TITLE

Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples

AUTHOR(S)
Prasad, Dinesh; Shankaracharya; Vidyarthi, Ambarish Sharan
PUB. DATE
September 2011
SOURCE
World Journal of Microbiology & Biotechnology;Sep2011, Vol. 27 Issue 9, p2227
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel with the conventional culture method. The sensitivity and specificity of colorimetric assay was 89.15 and 99.04% respectively with reference to conventional culture method. The total time required to detect the Salmonella spp. present in food samples by the developed method is less than 8 h, including 6 h incubation. It was observed that the colorimetric assay was 10 times more sensitive than gel-based detection with the same concentration of DNA used for analysis.
ACCESSION #
64128408

 

Related Articles

  • Usefulness of hepatitis C virus RNA counts by second generation HCV bDNA-probe in chronic hepatitis C based on the HCV genotype. Kobayashi, Mariko; Kumada, Hiromitsu; Arase, Yasuji; Chayama, Kazuaki; Kobayashi, Masahiro; Tsubota, Akihito; Koida, Isao; Saitoh, Satoshi; Suzuki, Yosiyuki; Murashima, Naoya; Ikeda, Kenji; Miyano, Yukiko; Mizoshita, Kaori; Matsuda, Marie; Koike, Hiromi; Hashimoto, Michie // Journal of Gastroenterology;1998, Vol. 33 Issue 2, p223 

    Abstract: Detection of hepatitis C virus (HCV) RNA by a second generation (ver 2) HCV bDNA-probe method (bDNA-probe) was compared with detection by the first generation (ver 1) assay. The two assays were performed simultaneously with the same serum samples of HCV genotypes 1b, 2a, 2b, 3a, and...

  • Microcantilever resonance-based DNA detection with nanoparticle probes. Su, Ming; Li, Shuyou; Dravid, Vinayak P. // Applied Physics Letters;5/19/2003, Vol. 82 Issue 20, p3562 

    Microcantilevers are used to detect DNA strands with a specific sequence using gold nanoparticle modified DNA. The hybridization reactions lead to the attachment of gold nanoparticles. After the amplification process by catalyzing the nucleation of silver, the shift of the cantilever frequency...

  • Assay speeds Salmonella detection.  // Emerging Food R&D Report;Feb2013, Vol. 23 Issue 11, p6 

    The article discusses the study of Jackson State University researchers regarding an assay to determine contamination of multiple-drug resistant (MDR) Salmonella. It states that the researchers use gold nanoparticles with antibodies which can detect other microbes. It mentions that the approach...

  • Microbiological Assays on Edible Seaweed Ulva Lactuca (L.) Cultured in Outdoor Tanks. KARACALAR, Ulviye; TURAN, Gamze // Journal of Applied Biological Sciences;May2008, Vol. 2 Issue 2, p27 

    In this study, microbiological analysis was performed on sun-dried Sea lettuce, Ulva lactuca (L.), cultured in outdoor seaweed tanks at Urla Marine Center of Ege University (Izmir, Turkey). A total of five microbial assays were performed between March and April 2005. Standard microbiological...

  • A Nonradioactive Assay for Poly(A)-Specific Ribonuclease Activity by Methylene Blue Colorimetry. Yuan Cheng; Wei-Feng Liu; Yong-Bin Yan; Hai-Meng Zhou // Protein & Peptide Letters;Feb2006, Vol. 13 Issue 2, p125 

    A simple nonradioactive assay, which was based on the specific shift of the absorbance maximum of methylene blue induced by its intercalation into poly(A) molecules, was developed for poly(A)-specific ribonuclease (PARN). A good linear relationship was found between the absorbance at 662 nm and...

  • NEW TRENDS ON MICROBIOLOGICAL WATER TREATMENT. Heidarpour, F.; Ghani, W. A.W. A. K.; Fakhru'l-Razi, A.; Sobri, S.; Torabian, A.; Heydarpour, V.; Zargar, M. // Digest Journal of Nanomaterials & Biostructures (DJNB);Apr-Jun2011, Vol. 6 Issue 2, p791 

    Silver nanoparticle-decorated porous polypropylene filter is prepared by physical vapor deposition method using a modified Balzers 760 coating machine. Silver nanoparticles were generated by electron beam bombardment of the silver metal. A 45nm layer of the silver nanoparticles were subsequently...

  • The ability of the BANA Test to detect different levels of P. gingivalis, T. denticola and T. forsythia. de Andrade, José Alexandre; Feres, Magda; de Figueiredo, Luciene Cristina; Salvador, Sérgio Luiz; Cortelli, Sheila Cavalca // Brazilian Oral Research;Apr-Jun2010, Vol. 24 Issue 2, p224 

    The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with...

  • Use of Activity-Based Probes to Develop High Throughput Screening Assays That Can Be Performed in Complex Cell Extracts. Deu, Edgar; Zhimou Yang; Flora Wang; Klemba, Michael; Bogyo, Matthew // PLoS ONE;2010, Vol. 5 Issue 8, p1 

    Background: High throughput screening (HTS) is one of the primary tools used to identify novel enzyme inhibitors. However, its applicability is generally restricted to targets that can either be expressed recombinantly or purified in large quantities. Methodology and Principal Findings: Here, we...

  • Development of a real-time multiplex PCR assay for detection of viral pathogens of penaeid shrimp. Zhixun Xie; Liji Xie; Yaoshan Pang; Zhaofa Lu; Zhiqin Xie; Jianhua Sun; Xianwen Deng; Jiabo Liu; Xiaofei Tang; Mazhar Khan // Archives of Virology;Dec2008, Vol. 153 Issue 12, p2245 

    A real-time multiplex polymerase chain reaction (rtm-PCR) assay was developed and optimized to simultaneously detect three viral pathogens of shrimp in one reaction. Three sets of specific oligonucleotide primers for white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics