Calibration of optical tweezers with differential interference contrast signals

Capitanio, M.; Romano, G.; Ballerini, R.; Giuntini, M.; Pavone, F. S.; Dunlap, D.; Finzi, L.
April 2002
Review of Scientific Instruments;Apr2002, Vol. 73 Issue 4, p1687
Academic Journal
A comparison of different calibration methods for optical tweezers with the differential interference contrast (DIC) technique was performed to establish the uses and the advantages of each method. A detailed experimental and theoretical analysis of each method was performed with emphasis on the anisotropy involved in the DIC technique and the noise components in the detection. Finally, a time of flight method that permits the reconstruction of the optical potential well was demonstrated.


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