TITLE

Morphologic Change and Elevation of Cortisol Secretion in Cultured Human Normal Adrenocortical Cells Caused by Mutant p21[sup K-ras] Protein

AUTHOR(S)
Wu, Chia-Hung; Lee, Su-Chen; Chiu, Hua-Hsien; Yang, Yuan-Chieh; Lian, Shen-Tsuen; Shin, Shyi-Jang; Lin, Shiu-Ru
PUB. DATE
January 2002
SOURCE
DNA & Cell Biology;Jan2002, Vol. 21 Issue 1, p21
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In our previous study on the tumorigenesis of human functional adrenal tumors, we observed a high frequency of K-ras point mutations in clinical specimens. Furthermore, we cloned the mutated K-ras gene from the tumors and inserted it into vectors to transfect normal bovine adrenocortical cells to express the mutated K-ras gene. The mRNA level of steroidogenic enzymes such as cholesterol sidechain cleavage enzyme (P450[sub SCC]), 17a-hydroxylase/17,20-lyase (P450[sub c17]), and 3�-hydroxysteroid dehydrogenase (3�HSD) in the mutant K-ras stably transfected cells were elevated. Cultured normal adrenocortical cells from donors and patients with adrenocortical tumors were then transfected with mutant K-ras expression plasmids constructed from human adrenal tumors. Stable transfectants grew faster than normal cells. Additionally, morphologic change was observed in the transfected cells. Moreover, when the synthesis of hormones was analyzed, the mRNA of P450[sub SCC], P450[sub C17], and 3�HSD was found to have increased, and the level of cortisol was 18 to 25 times that in control cells. The increased steroid hormone production in mutant K-ras-transfected cells was reversed by lovastatin, a pharmacologic inhibitor of p21[sup ras] function. These results, combined with previous reports of steroidogenic K-ras in bovine adrenocortical cells, suggest that the K-ras oncogene is involved in steroidogenesis in human adrenocortical cells.
ACCESSION #
6359144

 

Related Articles

  • Adrenal incidentalomas: Incidental in detection, not significance. Ooi, Teik Chey // CMAJ: Canadian Medical Association Journal;10/01/97, Vol. 157 Issue 7, p903 

    Editorial. Looks at the use of advanced imaging techniques as being reason for the finding of unsuspected adrenal tumors in investigations unrelated to the adrenal gland. The detection of serendipitous tumors called adrenal incidentalomas; The 80 percent that are benign or homonally silent;...

  • ERRATUM.  // Indian Journal of Endocrinology & Metabolism;Nov/Dec2013, Vol. 17 Issue 6, p982 

    A correction to the article "Feminising adrenal tumors: Our experience about 3 cases and literature review," by F. Chentli and colleagues published in a 2013 issue of the "Indian Journal of Endocrinology and Metabolism" is presented.

  • Adrenal Cortical Carcinoma: An Updated Review. Nayak, Samir R.; Soren, Dilip K.; Mishra, Aninidta; Mohapatra, Manisha; Srinivas, Ayappa // Internet Journal of Endocrinology;2013, Vol. 8 Issue 1, p1 

    Distant metastasis from differentiated thyroid carcinoma is a rare manifestation. Follicular thyroid cancer rarely manifests itself as a distant metastatic lesion. [1]. We report a 67 year old woman with no history of thyroid disorders referred for lump over the chest wall with clinical...

  • ADRENAL INCIDENTALOMA CASES: CLINICAL, LABORATORY AND IMAGING CHARACTERISTICS OF 62 PATIENTS. Emral, Rifat; Tonyukuk, Vedia; �n�r, Defne; �orap�io&gcaron;lu, Demet; Aydintu&gcaron;, Semih; Uysal, Ali Riza; Kamel, Nuri; Erdo&gcaron;an, G�rb�z // Journal of Ankara Medical School;Jun2003, Vol. 25 Issue 2, p55 

    Discusses the cases of patients with adrenal incidentalomas. Detection of adrenal tumors by abdominal ultrasonography, abdominal computed tomography (CT) or thoracic CT; Evaluation of pituitary-adrenal function.

  • Incidental adrenal mass found on CT scan. Kuraganti, Gayatri; Lee, Stephanie // Endocrine Today;Dec2011, Vol. 9 Issue 12, p30 

    The article focuses on adrenal incidentaloma (AI), an accidentally discovered adrenal mass on a radiological examination for an unrelated symptom, in a 43-year-old woman.

  • Anxiety and a severe headache. Sheahan, William T.; Saunders, Carol S. // Patient Care;9/30/1999, Vol. 33 Issue 15, p112 

    Describes the medical case of a 31-year-old nursing student afflicted with pheochromocytoma, a tumor of chromaffin cells and often seen in the adrenal glands. Clinical characteristics presented by the patient; Diagnostic and laboratory tests performed; Performance of exploratory laparotomy;...

  • Case 8.  // Journal of Endourology;Sep2008, Vol. 22 Issue 9, p1945 

    A 67-Year-Old Male Is Found to Have a 6-cm Pheochromocytoma of the Left Adrenal Gland on a Work-Up for Hypertension. Is There a Role for “Needlescopic” Instrumentation in the Laparoscopic Approach to This Case

  • Hormonal assessment in clinically silent adrenal pheochromocytoma. Shakiba, Behnam // Canadian Urological Association Journal;Apr2013, Vol. 7 Issue 3/4, p86 

    A letter to the editor is presented in response to the article "A case of clinically silent giant right pheochromocytoma and review of literature" by C. Li and colleagues in the June 2012 issue.

  • Incidentally Identified Adrenal Lipoma: Case Report and Review of Related Literature. Nathan Shumaker; Carrie Rochman; Robin Legallo; C. Northup; John Hanks // Endocrine Practice; 

    Objective: To report a case of an incidentally identified adrenal lipoma in a 68-year-old man with flank pain and hypertension.Methods: The clinical, computed tomographic, and magnetic resonance imaging findings in this patient are described, and the pathologic features of the tumor are...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics