Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: Public Health significance

Kankya, Clovice; Muwonge, Adrian; Djønne, Berit; Munyeme, Musso; Opuda-Asibo, John; Skjerve, Eystein; Oloya, James; Edvardsen, Vigdis; Johansen, Tone B.
January 2011
BMC Public Health;2011 Supplement 4, Vol. 11 Issue Suppl 4, p320
Academic Journal
Background: The importance of non-tuberculous mycobacteria (NTM) infections in humans and animals in sub- Saharan Africa at the human-environment-livestock-wildlife interface has recently received increased attention. NTM are environmental opportunistic pathogens of humans and animals. Recent studies in pastoral ecosystems of Uganda detected NTM in humans with cervical lymphadenitis and cattle with lesions compatible with bovine tuberculosis. However, little is known about the source of these mycobacteria in Uganda. The aim of this study was to isolate and identify NTM in the environment of pastoral communities in Uganda, as well as assess the potential risk factors and the public health significance of NTM in these ecosystems. Method: A total of 310 samples (soil, water and faecal from cattle and pigs) were examined for mycobacteria. Isolates were identified by the INNO-Lipa test and by 16S rDNA sequencing. Additionally, a questionnaire survey involving 231 pastoralists was conducted during sample collection. Data were analysed using descriptive statistics followed by a multivariable logistic regression analysis. Results: Forty-eight isolates of NTM were detected; 25.3% of soil samples, 11.8% of water and 9.1% from animal faecal samples contained mycobacteria. Soils around water sources were the most contaminated with NTM (29.8%). Of these samples, M. fortuitum-peregrinum complex, M. avium complex, M. gordonae, and M. nonchromogenicum were the most frequently detected mycobacteria. Drinking untreated compared to treated water (OR = 33), use of valley dam versus stream water for drinking and other domestic use (OR = 20), sharing of water sources with wild primates compared to antelopes (OR = 4.6), sharing of water sources with domestic animals (OR = 5.3), and close contact with cattle or other domestic animals (OR = 13.8) were the most plausible risk factors for humans to come in contact with NTM in the environment. Conclusions: The study detected a wide range of potentially pathogenic NTM from the environment around the pastoral communities in Uganda. Drinking untreated water and living in close contact with cattle or other domestic animals may be risk factors associated with the possibility of humans and animals acquiring NTM infections from these ecosystems.


Related Articles

  • Prevalence study on bovine tuberculosis and molecular characterization of its causative agents in cattle slaughtered at Addis Ababa municipal abattoir, Central Ethiopia. Mekibeb, Abraham; Fulasa, Tadele; Firdessa, Rebuma; Hailu, Elena // Tropical Animal Health & Production;Mar2013, Vol. 45 Issue 3, p763 

    A cross-sectional study was conducted on 500 cattle slaughtered at Addis Ababa abattoir to determine the prevalence of bovine tuberculosis (BTB) and characterize its causative agents. Postmortem examination, mycobacteriological culturing, region of difference-4 (RD4)-based PCR and spoligotyping...

  • Mycobacteria species in wild mammals of the Pantanal of central South America. Gomes Albertti, Letícia Alves; Souza-Filho, Antonio Francisco; Fonseca-Júnior, Antônio Augusto; Freitas, Michele Eduardo; de Oliveira-Pellegrin, Aiesca; Pinheiro Zimmermann, Namor; Walfrido Moraes Tomás; Fuzeta Schabib Péres, Igor Alexandre Hany; Fontana, Isabella; Rosa Osório, Ana Luiza Alves // European Journal of Wildlife Research;Feb2015, Vol. 61 Issue 1, p163 

    Bovine tuberculosis is a chronic disease of economic importance in livestock, but may also infect wild animals and occasionally humans. Its principal infective agent is Mycobacterium bovis. The present study was undertaken to examine the possible involvement of wildlife in the M. bovis life...

  • TB CRISIS DEEPENS AS BADGER LOBBIES AND DEFRA EXACERBATE IT.  // Farmers Weekly;4/12/2002, Vol. 136 Issue 15, p5 

    Assesses the condition of tuberculosis in cattle in Great Britain as of April 12, 2002. Criticism of the efforts of animal welfare groups to allow the slaughter of infected cattle; Objections against the apathetic response of the government to the disease; Implications of the apathy for British...

  • Steady rise in bovine TB but no emergency cull. Davies, Isabel // Farmers Weekly;5/3/2002, Vol. 136 Issue 18, p6 

    Reports that the Krebs trial, which seeks to address questions about the links between bovine tuberculosis and badgers and cattle in Great Britain, will proceed as planned despite a sharp increase in the number of herd breakdowns. Anticipated increase in tuberculosis in cattle in 2002; Another...

  • TB restrictions cause increasing frustration. Curtis, Marianne; Buss, Jessica // Farmers Weekly;8/30/2002, Vol. 137 Issue 9, p31 

    Reports on the frustration of cattle producers on the tuberculosis restrictions imposed by the British government. Total number of cows that are under the tuberculosis restrictions; Statement from veterinarian Chris Watson; Problems posed by the restrictions to beef herds selling stores.

  • Reduce suffering by supporting cull. Carter, Neil // Farmers Weekly;8/12/2011, Vol. 156 Issue 7, p34 

    The author asserts that supporting the cull plan can lessen the suffering of badgers infected with tuberculosis (TB).

  • Badger culling pilots. Jones, Mark; Allen, Caroline; Dalzell, Fiona; Eastwood, Bronwen; Edward, Richard; Elliott, Phill; Knight, Andrew; Lewis, Jo; McGill, Iain; MacMillan, Alastair; Menache, Andre; Southgate, Peter // Veterinary Record: Journal of the British Veterinary Association;07/20/2013, Vol. 173 Issue 2, p54 

    A letter to the editor is presented in response to an article about the bovine tuberculosis (TB) awareness in Great Britain in the June 8, 2013 issue.

  • What to expect if results are positive. Buss, Jessica // Farmers Weekly;8/9/2002, Vol. 137 Issue 6, p40 

    Details the process of clearing cattle affected by tuberculosis in Great Britain. Separation of affected cattle from the herd; Testing of the rest of the herd for TB; Delay in the receipt of official lesion testing result by breeders.

  • Bovine tuberculosis in South Darfur State, Sudan: an abattoir study based on microscopy and molecular detection methods. Asil, El; Sanousi, Sulieman; Gameel, Ahmed; Beir, Haytham; Fathelrahman, Maha; Terab, Nasir; Muaz, Magzoub; Hamid, Mohamed // Tropical Animal Health & Production;Feb2013, Vol. 45 Issue 2, p469 

    Bovine tuberculosis (BTB) is a widespread zoonosis in developing countries but has received little attention in many sub-Saharan African countries including Sudan and particularly in some parts such as Darfur states. This study aimed to detect bovine tuberculosis among caseous materials of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics