TITLE

Do mitochondria play a role in remodelling lace plant leaves during programmed cell death?

AUTHOR(S)
Christina E. N. Lord; Jaime N. Wertman; Stephanie Lane; Arunika H. L. A. N Gunawardena
PUB. DATE
January 2011
SOURCE
BMC Plant Biology;2011, Vol. 11 Issue 1, p102
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Programmed cell death (PCD) is the regulated death of cells within an organism. The lace plant (Aponogeton madagascariensis) produces perforations in its leaves through PCD. The leaves of the plant consist of a latticework of longitudinal and transverse veins enclosing areoles. PCD occurs in the cells at the center of these areoles and progresses outwards, stopping approximately five cells from the vasculature. The role of mitochondria during PCD has been recognized in animals; however, it has been less studied during PCD in plants. Results: The following paper elucidates the role of mitochondrial dynamics during developmentally regulated PCD in vivo in A. madagascariensis. A single areole within a window stage leaf (PCD is occurring) was divided into three areas based on the progression of PCD; cells that will not undergo PCD (NPCD), cells in early stages of PCD (EPCD), and cells in late stages of PCD (LPCD). Window stage leaves were stained with the mitochondrial dye MitoTracker Red CMXRos and examined. Mitochondrial dynamics were delineated into four categories (M1-M4) based on characteristics including distribution, motility, and membrane potential (Δψm). A TUNEL assay showed fragmented nDNA in a gradient over these mitochondrial stages. Chloroplasts and transvacuolar strands were also examined using live cell imaging. The possible importance of mitochondrial permeability transition pore (PTP) formation during PCD was indirectly examined via in vivo cyclosporine A (CsA) treatment. This treatment resulted in lace plant leaves with a significantly lower number of perforations compared to controls, and that displayed mitochondrial dynamics similar to that of non-PCD cells. Conclusions: Results depicted mitochondrial dynamics in vivo as PCD progresses within the lace plant, and highlight the correlation of this organelle with other organelles during developmental PCD. To the best of our knowledge, this is the first report of mitochondria and chloroplasts moving on transvacuolar strands to form a ring structure surrounding the nucleus during developmental PCD. Also, for the first time, we have shown the feasibility for the use of CsA in a whole plant system. Overall, our findings implicate the mitochondria as playing a critical and early role in developmentally regulated PCD in the lace plant.
ACCESSION #
62641902

 

Related Articles

  • The pathway of cell dismantling during programmed cell death in lace plant (Aponogeton madagascariensis) leaves. Wertman, Jamie; Lord, Christina EN; Dauphinee, Adrian N.; Gunawardena, Arunika HLAN // BMC Plant Biology;2012, Vol. 12 Issue 1, p115 

    Background: Developmentally regulated programmed cell death (PCD) is the controlled death of cells that occurs throughout the life cycle of both plants and animals. The lace plant (Aponogeton madagascariensis) forms perforations between longitudinal and transverse veins in spaces known as...

  • Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis. Lord, Christina E. N.; Gunawardena, Arunika H. L. A. N. // Planta;Feb2011, Vol. 233 Issue 2, p407 

    Within plant systems, two main forms of programmed cell death (PCD) exist: developmentally regulated and environmentally induced. The lace plant ( Aponogeton madagascariensis) naturally undergoes developmentally regulated PCD to form perforations between longitudinal and transverse veins over...

  • Erratum to: Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis. Lord, Christina; Gunawardena, Arunika // Planta;Mar2013, Vol. 237 Issue 3, p917 

    A correction to the article "Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis," by Christina E. N. Lord and Arunika H. L. A. N. Gunawardena, which was published online on December 15, 2012 is presented.

  • Cyclosporin A inhibits programmed cell death and cytochrome c release induced by fusicoccin in sycamore cells. R. Cerana; P. Crosti; M. Malerba // Protoplasma;Oct2007, Vol. 231 Issue 3/4, p193 

    Summary.  Programmed cell death plays a vital role in normal plant development, response to environmental stresses, and defense against pathogen attack. Different types of programmed cell death occur in plants and the involvement of mitochondria is still under investigation. In...

  • Two distinct signaling pathways regulate peroxynitrite-induced apoptosis in PC12 cells. Shacka, J. J.; Sahawneh, M. A.; Gonzalez, J. D.; Ye, Y.-Z.; D'Alessandro, T. L.; Estévez, A. G. // Cell Death & Differentiation;Sep2006, Vol. 13 Issue 9, p1506 

    The mechanisms of peroxynitrite-induced apoptosis are not fully understood. We report here that peroxynitrite-induced apoptosis of PC12 cells requires the simultaneous activation of p38 and JNK MAP kinase, which in turn activates the intrinsic apoptotic pathway, as evidenced by Bax translocation...

  • Programmed cell death and tissue remodelling in plants. Gunawardena, Arunika H. L. A. N. // Journal of Experimental Botany;Feb2008, Vol. 59 Issue 3, p445 

    The use of programmed cell death (PCD) to remodel plants at the cellular, tissue, and organ levels is particularly fascinating and occurs in such processes as tracheary element differentiation, lysigenous aerenchyma formation, development of functionally unisexual flowers from bisexual floral...

  • Mitochondrial Damage: A Target for New Therapeutic Horizons Soustiel, Jean F.; Larisch, Sarit // Neurotherapeutics;Jan2010, Vol. 7 Issue 1, p13 

    Summary: Traumatic brain injury (TBI) represents a leading cause of death and morbidity, as well as a considerable social and economical burden in western countries, and has thus emerged as a formidable therapeutic challenge. Yet despite tremendous efforts enlightening the mechanisms of neuronal...

  • Antamanide, a Derivative of Amanita phalloides, Is a Novel Inhibitor of the Mitochondrial Permeability Transition Pore. Azzolin, Luca; Antolini, Nicola; Calderan, Andrea; Ruzza, Paolo; Sciacovelli, Marco; Marin, Oriano; Mammi, Stefano; Bernardi, Paolo; Rasola, Andrea // PLoS ONE;2011, Vol. 6 Issue 1, p1 

    Antamanide is a cyclic decapeptide derived from the fungus Amanita phalloides. Here we show that antamanide inhibits the mitochondrial permeability transition pore, a central effector of cell death induction, by targeting the pore regulator cyclophilin D. Indeed, (i) permeability transition pore...

  • Erratum to: Prerequisites for ubiquinone analogs to prevent mitochondrial permeability transition-induced cell death. Belliere, Julie; Devun, Flavien; Cottet-Rousselle, Cécile; Batandier, Cécile; Leverve, Xavier; Fontaine, Eric // Journal of Bioenergetics & Biomembranes;Jun2012, Vol. 44 Issue 3, p397 

    A correction to the article "Prerequisites for ubiquinone analogs to prevent mitochondrial permeability transition-induced cell death," which was published in a 2012 issue is presented.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics