RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress

Marraccini, Pierre; Freire, Luciana P.; Alves, Gabriel S. C.; Vieira, Natalia G.; Vinecky, Felipe; Elbelt, Sonia; Ramos, Humberto J. O.; Montagnon, Christophe; Vieira, Luiz G. E.; Leroy, Thierry; Pot, David; Silva, Vânia A.; Rodrigues, Gustavo C.; Andrade, Alan C.
January 2011
BMC Plant Biology;2011, Vol. 11 Issue 1, p85
Academic Journal
Background: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure") genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for "pure" cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown in either greenhouse or field conditions under severe or moderate drought. However, this reduction of RBCS1 gene expression was not accompanied by a decrease in the corresponding protein in the leaves of C. canephora subjected to water withdrawal. In that case, the amount of RBCS1 was even higher under drought than under unstressed (irrigated) conditions, which suggests great stability of RBCS1 under adverse water conditions. On the other hand, for C. arabica, high nocturnal expression of RBCS1 could also explain the accumulation of the RBCS1 protein under water stress. Altogether, the results presented here suggest that the content of RBCS was not responsible for the loss of photosynthetic capacity that is commonly observed in water-stressed coffee plants. Conclusion: We showed that the CaCe homeolog was expressed in C. eugenioides and non-introgressed ("pure") genotypes of C. arabica but that it was undetectable in C. canephora. On the other hand, the CaCc homeolog was expressed in C. canephora but highly repressed in C. arabica. Expression of the CaCc homeolog was enhanced in C. arabica cultivars that experienced recent introgression with C. canephora. For both C. canephora and C. arabica species, total RBCS1 gene expression was highly reduced with WS. Unexpectedly, the accumulation of RBCS1 protein was observed in the leaves of C. canephora under WS, possibly coming from nocturnal RBCS1 expression. These results suggest that the increase in the amount of RBCS1 protein could contribute to the antioxidative function of photorespiration in water-stressed coffee plants.


Related Articles

  • Regulation of gene expression by photosynthetic signals triggered through modified CO2 availability. Wormuth, Dennis; Baier, Margarete; Kandlbinder, Andrea; Scheibe, Renate; Hartung, Wolfram; Dietz, Karl-Josef // BMC Plant Biology;2006, Vol. 6, p1 

    Background: To coordinate metabolite fluxes and energy availability, plants adjust metabolism and gene expression to environmental changes through employment of interacting signalling pathways. Results: Comparing the response of Arabidopsis wild-type plants with that of the mutants adg1, pgr1...

  • Response of Photosynthesis in Maize to Drought and Re-Watering. Liu, J.; Guo, Y. Y.; Bai, Y. W.; Li, H. J.; Xue, J. Q.; Zhang, R. H. // Russian Journal of Plant Physiology;May2019, Vol. 66 Issue 3, p424 

    The mechanism by which photosynthetic adaptation occurs in maize (Zea mays L.) during both drought and the subsequent recovery after re-watering is currently unknown. To elucidate this mechanism, two maize cultivars (drought tolerant SD609 and drought sensitive SD902) were subjected to an...

  • Comparison of Drought Stress Response and Gene Expression between a GM Maize Variety and a Near-Isogenic Non-GM Variety. Gullì, Mariolina; Salvatori, Elisabetta; Fusaro, Lina; Pellacani, Claudia; Manes, Fausto; Marmiroli, Nelson // PLoS ONE;Feb2015, Vol. 10 Issue 2, p1 

    Maize MON810, grown and commercialised worldwide, is the only cultivated GM event in the EU. Maize MON810, variety DKC6575, and the corresponding near-isogenic line Tietar were studied in different growth conditions, to compare their behaviour in response to drought. Main photosynthetic...

  • Photosynthetic entrainment of the Arabidopsis thaliana circadian clock. Haydon, Michael J.; Mielczarek, Olga; Robertson, Fiona C.; Hubbard, Katharine E.; Webb, Alex A. R. // Nature;10/31/2013, Vol. 502 Issue 7473, p689 

    Circadian clocks provide a competitive advantage in an environment that is heavily influenced by the rotation of the Earth, by driving daily rhythms in behaviour, physiology and metabolism in bacteria, fungi, plants and animals. Circadian clocks comprise transcription-translation feedback loops,...

  • Trafficking of Plant Vacuolar Invertases: From a Membrane-Anchored to a Soluble Status. Understanding Sorting Information in Their Complex N-Terminal Motifs. Xiang, Li; Van den Ende, Wim // Plant & Cell Physiology;Aug2013, Vol. 54 Issue 8, p1263 

    Vacuolar invertases (VIs) are highly expressed in young tissues and organs. They may have a substantial regulatory influence on whole-plant metabolism as well as on photosynthetic efficiency. Therefore, they are emerging as potentially interesting biotechnological targets to increase plant...

  • Drying without senescence in resurrection plants. Griffiths, Cara A.; Gaff, Donald F.; Neale, Alan D. // Frontiers in Plant Science;Feb2014, Vol. 5, p1 

    Research into extreme drought tolerance in resurrection plants using species such as Craterostigma plantagineum, C. wilmsii, Xerophyta humilis, Tortula ruralis, and Sporobolus stapfianus has provided some insight into the desiccation tolerance mechanisms utilized by these plants to allow them to...

  • Roles of Gibberellin Catabolism and Signaling in Growth and Physiological Response to Drought and Short-Day Photoperiods in Populus Trees. Zawaski, Christine; Busov, Victor B. // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Survival and productivity of perennial plants in temperate zones are dependent on robust responses to prolonged and seasonal cycles of unfavorable conditions. Here we report whole-genome microarray, expression, physiological, and transgenic evidence in hybrid poplar (Populus tremula ×...

  • Transcriptome Changes in Hirschfeldia incana in Response to Lead Exposure. Auguy, Florence; Fahr, Mouna; Moulin, Patricia; El Mzibri, Mohamed; Smouni, Abdelaziz; Filali-Maltouf, Abdelkarim; Béna, Gilles; Doumas, Patrick // Frontiers in Plant Science;Jan2016, Vol. 6, p1 

    Hirschfeldia incana, a pseudometallophyte belonging to the Brassicaceae family and widespread in the Mediterranean region, was selected for its ability to grow on soils contaminated by lead (Pb). The global comparison of gene expression using microarrays between a plant susceptible to Pb...

  • The Coordination of Gene Expression within Photosynthesis Pathway for Acclimation of C4 Energy Crop Miscanthus lutarioriparius. Shilai Xing; Lifang Kang; Qin Xu; Yangyang Fan; Wei Liu; Caiyun Zhu; Zhihong Song; Qian Wang; Juan Yan; Jianqiang Li; Tao Sang; Xinguang Zhu; Chengbin Xiang // Frontiers in Plant Science;2/9/16, p1 

    As a promising candidate for the second-generation C4 energy crop, Miscanthus lutarioriparius has well acclimated to the water-limited and high-light Loess Plateau in China by improving photosynthesis rate and water use efficiency (WUE) compared to its native habitat along Yangtze River....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics