TITLE

Gene expression profile analysis of tobacco leaf trichomes

AUTHOR(S)
Hong Cui; Song-Tao Zhang; Hui-Juan Yang; Hao Ji; Xiu-Jie Wang
PUB. DATE
January 2011
SOURCE
BMC Plant Biology;2011, Vol. 11 Issue 1, p76
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Leaf trichomes of Nicotiana tabacum are distinguished by their large size, high density, and superior secretion ability. They contribute to plant defense response against biotic and abiotic stress, and also influence leaf aroma and smoke flavor. However, there is limited genomic information about trichomes of this non-model plant species. Results: We have characterized Nicotiana tabacum leaf trichome gene expression using two approaches. In the first, a trichome cDNA library was randomly sequenced, and 2831 unique genes were obtained. The most highly abundant transcript was ribulose bisphosphate carboxylase (RuBisCO). Among the related sequences, most encoded enzymes involved in primary metabolism. Secondary metabolism related genes, such as isoprenoid and flavonoid biosynthesis-related, were also identified. In the second approach, a cDNA microarray prepared from these 2831 clones was used to compare gene expression levels in trichome and leaf. There were 438 differentially expressed genes between trichome and leaves-minus-trichomes. Of these, 207 highly expressed genes in tobacco trichomes were enriched in second metabolic processes, defense responses, and the metabolism regulation categories. The expression of selected unigenes was confirmed by semi-quantitative RT-PCR analysis, some of which were specifically expressed in trichomes. Conclusion: The expression feature of leaf trichomes in Nicotiana tabacum indicates their metabolic activity and potential importance in stress resistance. Sequences predominantly expressed in trichomes will facilitate genemining and metabolism control of plant trichome.
ACCESSION #
62639449

 

Related Articles

  • Expression Profiling of Tobacco Leaf Trichomes Identifies Genes for Biotic and Abiotic Stresses. Harada, Emiko; Ji-Ah Kim; Meyer, Andreas J.; Hell, Rüdiger; Clemens, Stephan; Yong-Eui Cho // Plant & Cell Physiology;Oct2010, Vol. 51 Issue 10, p1627 

    Nicotiana tabacum (tobacco) plants have short and long glandular trichomes. There is evidence that tobacco trichomes play several roles in the defense against biotic and abiotic stresses. cDNA libraries were constructed from control and cadmium (Cd)-treated leaf trichomes. Almost 2,000 expressed...

  • The family of LSU-like proteins. Sirko, Agnieszka; Wawrzyńska, Anna; Collados Rodríguez, Milagros; Sęktas, Pawel // Frontiers in Plant Science;Jan2015, Vol. 5, p1 

    The plant response to sulfur deficiency includes extensive metabolic changes which can be monitored at various levels (transcriptome, proteome, metabolome) even before the first visible symptoms of sulfur starvation appear. Four members of the plant-specific LSU (response to Low SUlfur) gene...

  • Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells. Andreas Weise; Sylvie Lalonde; Christina K�hn; Wolf Frommer; John Ward // Plant Molecular Biology;Oct2008, Vol. 68 Issue 3, p251 

    Abstract��In solanaceous plants such as tomato and tobacco, the sucrose transporter SUT1 is crucial for phloem loading. Using GUS as a reporter, the promoter and other regulatory cis elements required for the tomato LeSUT1 expression were analyzed by heterologous expression of...

  • Trichome expression of iaaM transgene influences their development and elongation in tobacco. Zhao, Y.; Huang, L.; Peng, Y.; Peng, Z.; Liu, X.; Kuang, F.; Zhang, X. // Russian Journal of Plant Physiology;Nov2013, Vol. 60 Issue 6, p839 

    The iaaM gene encodes a monooxygenase, an enzyme that can catalyze the synthesis of IAA from tryptophan and is functional in plants. We cloned the iaaM into the T-DNA region of a Ti binary vector pWM101 under the control of the cotton fiber specific E6 promoter, and the recombinant was...

  • Parallel Domestication, Convergent Evolution and Duplicated Gene Recruitment in Allopolyploid Cotton. Hovav, Ran; Chaudhary, Bhupendra; Udall, Joshua A.; Flagel, Lex; Wendel, Jonathan F. // Genetics;Jul2008, Vol. 179 Issue 3, p1725 

    A putative advantage of allopolyploidy is the possibility of differential selection of duplicated (homeologous) genes originating from two different progenitor genomes. In this note we explore this hypothesis using a high throughput, SNP-specific microarray technology applied to seed trichomes...

  • Metabolism: Athero: all but Sirt-ain? Levinson, Randy // Nature Medicine;Jan2012, Vol. 18 Issue 1, p34 

    The article focuses on the role of genetic overexpression of sirtuin (silent mating type information regulation 2 homolog) 1 (Sirt1) in improving the macrophage cholesterol metabolism in mice and in preventing the development of atherosclerosis, based from the study of Li Qiang and colleagues.

  • Nature Communications: Molecular economics: New computer models calculate systems-wide costs of gene expression.  // Biomedical Market Newsletter;8/1/2012, Vol. 21, p1 

    The article informs that bioengineers are developing a model to study the association between metabolism and gene expression in larger organisms.

  • Anaerobic induction of the maize GapC4 promoter in poplar leaves requires light and high CO 2. Robert Hänsch; Tobias Kurz; Jutta Schulze; Ralf R. Mendel; Rüdiger Cerff; Reinhard Hehl // Planta;Nov2003, Vol. 218 Issue 1, p79 

    The maize ( Zea mays L.) glyceraldehyde-3-phosphate dehydrogenase gene 4 ( GapC4) promoter confers anaerobic gene expression in tobacco ( Nicotiana tabacum L.), potato ( Solanum tuberosum L.) and Arabidopsis thaliana (L.) Heynh. Here we have investigated its expression in hybrid poplar ( Populus...

  • Intron-mediated enhancement of gene expression in transgenic plants using chimeric constructs composed of the Peanut chlorotic streak virus (PClSV) promoter?leader and the antisense orientation of PClSV ORF VII (p7R). Somnath Bhattacharyya; Sitakanta Pattanaik; Indu B. Maiti // Planta;Nov2003, Vol. 218 Issue 1, p115 

    The antisense orientation of the Peanut chlorotic streak virus (PClSV) open reading frame (ORF) VII (denoted as p7R), in conjunction with the sense orientation of the PClSV leader sequence, acts as an intron and enhances the expression of a reporter gene, analyzed in protoplasts and transgenic...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics