TITLE

HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND STAVUDINE IN BULK AND COMBINED TABLET DOSAGE FORM

AUTHOR(S)
Kumaraswamy, G.; Rajendra Kumar, J. M.; Sheshagiri Rao, J. V. L. N.; Vinay Kumar, D.; Prabakar, D.; Ashok Kumar, U.
PUB. DATE
July 2011
SOURCE
International Journal of Pharmacy & Pharmaceutical Sciences;Jul2011, Vol. 3 Issue 3, p142
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A Simple, accurate and precise HPLC method was developed for the simultaneous estimation of Lamivudine and Stavudine in bulk and pharmaceutical dosage forms. The quantification was carried out using symmetry C-18, Thermo (100x4.6,), i.d, and 3.5 μm particle size in isocratic mode, with mobile phase compressing of Phosphate buffer pH 2.5: Methanol (80:20v/v). The flow rate was 0.8 ml/min and the detection was carried out by UV detector dual i.e. wavelength of 262nm for Stavudine and 271nm for Lamivudine was selected. The retention time for Lamivudine and Stavudine was found to be 2.16 and 4.32 min, respectively. The method was found to be linear in the range of 25-125 μg/ml for Lamivudine and Stavudine. The developed method was validated in terms of accuracy, precision, LOD, LOQ, robustness and solution stability. The percentage recovery was found to be 98.73 and 99.15 % for Lamivudine and Stavudine respectively. The proposed method can be successfully used for the estimation of Lamivudine and Stavudine in bulk and combined dosage forms.
ACCESSION #
62639370

 

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