TITLE

The effect of passive smoking on the levels of matrix metalloproteinase 9 in nasal secretions of children

AUTHOR(S)
De, Sujata; Leong, Samuel C.; Fenton, John E.; Carter, Stuart D.; Clarke, Raymond W.; Jones, Andrew S.
PUB. DATE
July 2011
SOURCE
American Journal of Rhinology & Allergy;Jul/Aug2011, Vol. 25 Issue 4, p226
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Matrix metalloproteinase (MMP) 9 is a gelatinase associated with tissue remodeling. It is thought to play a part in the pathogenesis of allergy. Increased levels of MMP-9 have been shown to increase in the acute allergic response in the nose, lungs, and skin. Exposure to passive tobacco smoke is associated with an increase in sneezing, nasal blockage, and a decreased sense of smell. The aim of this study was to study the effect of passive smoking on the levels of MMP-9 in nasal secretions of children. Methods: A prospective descriptive study was performed. Thirty-nine children aged between 7 and 16 years were enrolled in the study. They were selected based on attendance at the Otorhinolaryngology Outpatients Clinic with a primary complaint unrelated to the nose or paranasal sinuses. Children with allergic rhinitis, sinusitis, or a recent cold were excluded. The study was performed at a tertiary pediatric referral center. Exposure to passive smoking was determined by measuring the urinary cotinine to creatinine ratio. Nasal fluid was obtained by using a Rhino-Probe curette (Arlington Scientific, Inc., Springville, UT). The concentration of MMP-9 was determined by ELISA. MMP-9 activity was determined by gelatin zymography. Data were tabulated on Microsoft Excel (Microsoft Corp., Redmond, WA) and analyzed using SPSS (SPSS Inc., Chicago, IL). Results: Using a cutoff urinary cotinine/creatinine ratio of 0.025 ng/mg, 15 children were found to be exposed to passive smoking. Both the MMP-9 concentration and the activity were significantly higher in nasal secretions of children exposed to passive smoking. There was a distinct difference between the two cohorts with regard to the level of enzyme activity per weight of protein. The lowest level of enzyme activity recorded in the 'exposed' cohort was over twice that of the level in the 'not exposed' cohort. Conclusion: MMP-9 activity and concentration is higher in nasal secretions of children exposed to passive smoking. This suggests that passive smoking might alter the inflammatory response within the nasal mucosa in a similar way to allergy.
ACCESSION #
62541141

 

Related Articles

  • Expression of extracellular matrix metalloproteinase inducer in nasal polyps. Lee, Jang-Hyeog; Chung, Seung Won; Park, Il-Ho; Lee, Sang Hag; Lee, Seung Hoon; Lee, Heung-Man // American Journal of Rhinology & Allergy;Nov/Dec2010, Vol. 24 Issue 6, p127 

    Background: Extracellular matrix metalloproteinase inducer (EMMPRIN) is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily. The presence of EMMPRIN in nontumoral tissues suggests a role in other physiological and pathological situations, which may be associated with...

  • Extra-Cellular Matrix Proteins Induce Matrix Metalloproteinase-1 (MMP-1) Activity and Increase Airway Smooth Muscle Contraction in Asthma. Rogers, Natasha K.; Clements, Debbie; Dongre, Arundhati; Harrison, Tim W.; Shaw, Dominic; Johnson, Simon R. // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    Airway remodelling describes the histopathological changes leading to fixed airway obstruction in patients with asthma and includes extra-cellular matrix (ECM) deposition. Matrix metalloproteinase-1 (MMP-1) is present in remodelled airways but its relationship with ECM proteins and the resulting...

  • Tissue Inhibitors of Metalloproteinases (TIMPs): Their Biological Functions and Involvement in Oral Disease. Verstappen, J.; den Hoff, J. W. Von // Journal of Dental Research;Dec2006, Vol. 85 Issue 12, p1074 

    Several families of enzymes are responsible for the degradation of extracellular matrix (ECM) proteins during the remodeling of tissues. An important family of such enzymes is that of the matrix metalloproteinases (MMPs). To control MMP-mediated ECM breakdown, tissue inhibitors of...

  • A time course study on the development of allergen-induced nasal airway remodeling in a rat model. Chung, Young-Jun; Kim, Hyo Yeol; Chung, Seung-Kyu; Dhong, Hun-Jong // American Journal of Rhinology & Allergy;Nov/Dec2012, Vol. 26 Issue 6, p421 

    Background: Only a few studies have investigated the airway remodeling process in allergic rhinitis (AR), and the results reported are conflicting. We established an allergen-induced nasal remodeling model for AR using brown Norway rats and investigated time-dependent histological changes and...

  • Matrix metalloproteinases: A review of their structure and role in acute coronary syndrome. Jones, Charles B.; Sane, David C.; Herrington, David M. // Cardiovascular Research;Oct2003, Vol. 59 Issue 4, p812 

    Objective: To summarize the structure, function, and regulation of matrix metalloproteinases (MMPs) and to review the literature to date on their potential role in the pathogenesis of acute coronary syndromes. Methods: A recursive strategy starting with a Medline Search for primary...

  • The importance of proline residues in the structure, stability and susceptibility to proteolytic degradation of collagens. Stephen Krane // Amino Acids;Nov2008, Vol. 35 Issue 4, p703 

    Abstract  Collagens are among proteins that undergo several post-translational modifications, such as prolyl hydroxylation, that occur during elongation of the nascent chains in the endoplasmic reticulum. The major structural collagens, types I, II and III, have large, uninterrupted triple...

  • The metabolism of proline, a stress substrate, modulates carcinogenic pathways. James Phang; Steven Donald; Jui Pandhare; Yongmin Liu // Amino Acids;Nov2008, Vol. 35 Issue 4, p681 

    Abstract  The resurgence of interest in tumor metabolism has led investigators to emphasize the metabolism of proline as a “stress substrate” and to suggest this pathway as a potential anti-tumor target. Proline oxidase, a.k.a. proline dehydrogenase (POX/PRODH), catalyzes the...

  • Age-dependent changes in myocardial matrix metalloproteinase/tissue inhibitor of metalloproteinase profiles and fibroblast function. Lindsey, Merry L.; Goshorn, Danielle K.; Squires, Christina E.; Escobar, G. Patricia; Hendrick, Jennifer W.; Mingoia, Joseph T.; Sweterlitsch, Sarah E.; Spinale, Francis G. // Cardiovascular Research;May2005, Vol. 66 Issue 2, p410 

    Abstract: Objective: To evaluate the effects of aging on left ventricular (LV) geometry, collagen levels, matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) abundance, and myocardial fibroblast function. Methods: Young (3-month-old; n=28), middle-aged (MA;...

  • The role of extracellular matrix metalloproteinases and their inhibitors in allergic diseases. Kuźmiński, Andrzej; Przybyszewski, Michał; Graczyk, Małgorzata; Bartuzi, Zbigniew // Advances in Dermatology & Allergology / Postepy Dermatologii i A;2012, Vol. 29 Issue 5, p384 

    During the migration to the site of inflammation, allergic inflammatory cells must overcome several structures that constitute an impregnable barrier under normal conditions. One such structure is the extracellular matrix responsible for closely filling the intercellular space of tissues. The...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics