TITLE

Development of 30 Novel Polymorphic Expressed Sequence Tags (EST)-Derived Microsatellite Markers for the Miiuy Croaker, Miichthys miiuy

AUTHOR(S)
Tianjun Xu; Dianqiao Sun; Yuena Sun; Rixin Wang
PUB. DATE
June 2011
SOURCE
International Journal of Molecular Sciences;Jun2011, Vol. 12 Issue 6, p4021
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2-13 alleles per locus. The observed and expected heterozygosities were from 0.1024 to 0.7917 and from 0.2732 to 0.8845, respectively. Nine loci deviated from the Hardy-Weinberg equilibrium, and linkage disequilibrium was significant between 22 pairs of loci. These polymorphic microsatellite loci will be useful for genetic diversity analysis and molecule-assisted breeding for M. miiuy.
ACCESSION #
62253021

 

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