Oligodeoxynucleotide Containing Disulphide Bond Stabilizes Triplex DNA Structure

Kumar, Ajay
April 2011
E-Journal of Chemistry;2011, Vol. 8 Issue 2, p507
Academic Journal
The triplex formation of disulphide containing oligonucleotide with duplex DNA using melting temperature studies is reported. The stability of the triplex formed with disulphide containing oligonucleotide was compared with unmodified oligonucleotide and C-5 propyne deoxyuridine containing oligonucleotide. Melting temperature (Tm) values of the triplexes formed with disulphide containing oligonucleotide were found to be 37 °C and 46 °C at pH 7 and 6 respectively. The triplexes formed with C-5 propyne deoxyuridine substituted oligonucleotide showed Tm values at 27 °C and 42 °C at pH 7 and 6. The Tm values of the triplexes formed with unmodified oligonucleotide were found to be 18 °C and 32 °C at pH 7 and 6 respectively. This clearly demonstrates that disulphide containing oligonucleotide stabilzes triplex DNA structure better than unmodified oligonucleotide as well as C-5 propyne deoxyuridine containing oligonucleotide when targeted with duplex DNA at both the mentioned pH values.


Related Articles

  • Antisense Technology: A Selective Tool for Gene Expression Regulation and Gene Targeting. Sahu, N. K.; Shilakari, G.; Nayak, A.; Kohli, D. V. // Current Pharmaceutical Biotechnology;Oct2007, Vol. 8 Issue 5, p291 

    This review deals with the antisense technology that, together, forms a very powerful tool to inhibit gene expression and may be used for studying gene function (functional genomics) and for therapeutic purpose (antisense gene therapy). Antisense oligonucleotides block translation of target...

  • Triplex-Forming Oligonucleotides as Potential Tools for Modulation of Gene Expression. Rogers, Faye A.; Lloyd, Jnice A.; Glazer, Peter M. // Current Medicinal Chemistry - Anti-Cancer Agents;Jul2005, Vol. 5 Issue 4, p319 

    Triplex-forming oligonucleotides (TFOs) bind in the major groove of duplex DNA at polypurine/ polypyrimidine stretches in a sequence-specific manner. The binding specificity of TFOs makes them potential candidates for use in directed genome modification. A number of studies have shown that TFOs...

  • Anti-Gene Strategies to Down-Regulate Gene Expression in Mammalian Cells. Cogoi, S.; Rapozzi, V.; Xodo, L. E. // Current Pharmaceutical Design;Mar2004, Vol. 10 Issue 7, p805 

    A current goal in molecular medicine is the development of new strategies for the selective inhibition of cancer-critical genes. Triplex-forming oligonucleotides and peptide nucleic acids bind to the double helix of DNA in a sequence-specific manner and with great affinity. Because of these...

  • The Cdk5-p35 kinase associates with the Golgi apparatus and regulates membrane traffic. Paglini, Gariela; Peris, Leticia; Diez-Guenra, Javier; Quiroga, Santiago; Cáceres, Aifredo // EMBO Reports;Dec2001, Vol. 2 Issue 12, p1139 

    We show here that an active Cdk5-p35 kinase is present in Golgi membranes, where it associates with a detergent-insoluble fraction containing actin. In addition, Cdk5-p35- dependent phosphorylation of α-PAK immunoreactive protein species was detected in Golgi membranes, as well as an...

  • Analysis of Perfect and Mismatched DNA Duplexes with a Generic Hexanucleotide Microchip. Khomyakova, E. B.; Livshits, M. A.; Sharonov, A. Yu.; Prokopenko, D. V.; Mirzabekov, A. D. // Molecular Biology;Jul/Aug2003, Vol. 37 Issue 4, p617 

    Thermodynamic analysis was performed for the duplexes formed by fluorescently labeled oligonucleotide targets on a generic hexanucleotide microchip. All 4096 different hexanucleotide chains were immobilized as probes in individual gel pads of the microchip. To strengthen the hybridization, each...

  • Clusterin confers gmcitabine resistance in pancreatic cancer. Qingfeng Chen; Zhengkun Wang; Kejun Zhang; Xiaoyi Liu; Weihong Cao; Lei Zhang; Shuhua Zhang; Bomin Yan; Yaoguang Wang; Chunping Xia // World Journal of Surgical Oncology;2011, Vol. 9 Issue 1, p59 

    Objective: To measure clusterin expression in pancreatic cancer tissues and cell lines and to evaluate whether clusterin confers resistance to gmcitabine in pancreatic cancer cells. Methods: Immunohistochemistry for clusterin was performed on 50 primary pancreatic cancer tissues and 25 matched...

  • A strategy for detection of known and unknown SNP using a minimum number of oligonucleotides applicable in the clinical settings. Wang, Ena; Adams, Sharon; Yingdong Zhao; Panelli, Monica; Simon, Richard; Klein, Harvey; Marincola, Francesco M. // Journal of Translational Medicine;2003, Vol. 1, p4 

    Detection of unknown single nucleotide polymorphism (SNP) relies on large scale sequencing expeditions of genomic fragments or complex high-throughput chip technology. We describe a simplified strategy for fluorimetric detection of known and unknown SNP by proportional hybridization to...

  • CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway. Jianfeng Zheng; Rongquan Fu; Jing Li; Xiaozhong Wang // Journal of Experimental & Clinical Cancer Research (17569966);2011, Vol. 30 Issue 1, p48 

    Objective: To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro. Methods: The...

  • Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases. Rogers, Faye A.; Lloyd, Janice A.; Tiwari, Meetu Kaushik // Artificial DNA;Jan2014, Vol. 5, p1 

    Triplex structures generated by sequence-specific triplex-forming oligonucleotides (TFOs) have proven to be promising tools for gene targeting strategies. In addition, triplex technology has been highly utilized to study the molecular mechanisms of DNa repair, recombination and mutagenesis....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics