TITLE

Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment

AUTHOR(S)
Khuc, Mai-Thu; Mendonça, Lucille; Sharma, Sapna; Solinas, Xavier; Volk, Martin; Hache, François
PUB. DATE
May 2011
SOURCE
Review of Scientific Instruments;May2011, Vol. 82 Issue 5, p054302
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution.
ACCESSION #
62010742

 

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