TITLE

Development of a new method for diagnosis of Group B Coxsackie genome by reverse transcription loop-mediated isothermal amplification

AUTHOR(S)
Jaianand, K.; Saravanan, N.; Gunasekaran, P.; Sheriff, A. K.
PUB. DATE
April 2011
SOURCE
Indian Journal of Medical Microbiology;Apr2011, Vol. 29 Issue 2, p110
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Coxsackie B viruses (genus, Enterovirus; family, Picornaviridae) can cause aseptic meningitis, encephalitis, pleurodynia, and fatal myocarditis, and are implicated in the pathogenesis of dilated cardiomyopathy. The differentiation of the group B Coxsackieviruses into their subtypes has potential clinical and epidemiological implications. Objective: In this study, we developed a one-step, single-tube genogroup-specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of group B Coxsackie genomes targeting 5′ UTR region. Materials and Methods: The amplification can be obtained in less than 1 hour by incubating all the reagents in a single tube with reverse transcriptase and Bst DNA polymerase at 63°C. Detection of gene amplification could be accomplished by agarose gel electrophoresis and the monitoring of gene amplifi cation can also be visualised with the naked eye by using SYBR green I fluorescent dye. Results: A total of 40 samples comprising 31 positive samples and 9 negative samples were used in this study for comparative evaluation. The results were compared with those from Real-Time Polymerase Chain Reaction (RT-PCR). None of the RT-PCR-positive samples were missed by RT-LAMP, thereby indicating a higher sensitivity of the RT-LAMP assay. Conclusion: Thus, due to easy operation without a requirement of sophisticated equipment and skilled personnel, the RT-LAMP assay reported here is extremely rapid, cost-effective, highly sensitive, and specifi c and has potential usefulness for rapid detection of non-polio enterovirus (NPEV) not only by well-equipped laboratories but also by peripheral diagnostic laboratories with limited fi nancial resources in developing countries.
ACCESSION #
61346944

 

Related Articles

  • Echoviruses are a major cause of aseptic meningitis in infants and young children in Kuwait. Dalwai, Ajmal; Ahmad, Suhail; Al-Nakib, Widad // Virology Journal;2010, Vol. 7, p236 

    Background: The etiologic agents of aseptic meningitis (AM) often include human enteroviruses. The role of enteroviruses causing AM in young children was investigated during a 3-year period in Kuwait. Results: Enteroviral RNA was detected in cerebrospinal fluid (CSF) by reverse transcription-PCR...

  • ENTEROVIRAL INFECTION OUTBREAK IN THE REPUBLIC OF BELARUS: PRINCIPAL CHARACTERISTICS AND PHYLOGENETIC ANALYSIS OF ETIOLOGICAL AGENTS. Amvrosieva, Tamara V.; Paklonskaya, Natallia V.; Biazruchka, Aliaksei A.; Kazinetz, Olga N.; Bohush, Zoya F.; Fisenko, Elena G. // Central European Journal of Public Health;2006, Vol. 14 Issue 2, p67 

    For the last decade enterovirus outbreaks were registered in all of six districts of Belarus. Two of them, reported in 1997 (in Gomel) and in 2003 (in Minsk), were the most extensive and involved 461 and 1,351 patients respectively. Virus ECHO 30 was identified as the dominant etiologic agent of...

  • A Broadly Cross-protective Vaccine Presenting the Neighboring Epitopes within the VP1 GH Loop and VP2 EF Loop of Enterovirus 71. Xu, Longfa; He, Delei; Yang, Lisheng; Li, Zhiqun; Ye, Xiangzhong; Yu, Hai; zhao, Huan; Li, Shuxuan; Yuan, Lunzhi; Qian, Hongliu; Que, Yuqiong; Kuo Shih, James Wai; Zhu, Hua; Li, Yimin; Cheng, Tong; Xia, Ningshao // Scientific Reports;8/7/2015, p12973 

    Human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are the major etiological agents of hand, foot and mouth disease (HFMD) and are often associated with neurological complications. Currently, several vaccine types are being developed for EV71 and CA16. In this study, we constructed a...

  • Novel and Predominant Pathogen Responsible for the Enterovirus-Associated Encephalitis in Eastern China. Zhang, Lei; Yan, Jie; Ojcius, David M.; Lv, Huakun; Miao, Ziping; Chen, Yin; Zhang, Yanjun; Yan, Jvying // PLoS ONE;Dec2013, Vol. 8 Issue 12, p1 

    Enteroviruses (EV) have been increasingly identified as the causative agent for unknown etiological encephalitis in many parts of the world, but the long period surveillance for enterovirus-associated encephalitis (EAE) was not reported in China. From 2002-2012 in Zhejiang, Coxsackieviruses A9,...

  • Enteroviral Infection of a Cardiac Prosthetic Device. Blumental, Sophie; Reynders, Marijke; Willems, Ariane; Biarent, Dominique; Duttman, Ruth; Lepage, Philippe; Vergison, Anne // Clinical Infectious Diseases;Mar2011, Vol. 52 Issue 6, p710 

    Case report of a trisomic infant with cardiac prosthetic device who presented three recurrences of culture negative endocarditis. Coxsackie virus was the only pathogen recovered from the endocardial tissue and its role as etiological agent of the infection is discussed.Background. Identification...

  • Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control. Xing-Long Xiao; Ya-Qing He; Yi-Gang Yu; Hong Yang; Gu Chen; Hui-Fang Li; Jing-Wei Zhang; Dong-Mei Liu; Xiao-Feng Li; Xiao-Quan Yang; Hui Wu // Archives of Virology;Jan2009, Vol. 154 Issue 1, p121 

    The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71...

  • Outbreak of febrile illness caused by coxsackievirus A4 in a nursery school in Beijing, China. Jin-Song Li; Xiao-Gen Dong; Meng Qin; Zhi-Ping Xie; Han-Chun Gao; Jun-Yong Yang; Xiao-Xin Yang; Dan-Di Li; Jie Li; Zhao-Jun Duan // Virology Journal;Jul2015, Vol. 12 Issue 1, p1 

    Background: Coxsackievirus A4 (CV-A4) is classified as human enterovirus A according to its serotype. CV-A4, an etiological agent of hand, foot, and mouth disease, affects children worldwide and can circulate in closed environments such as schools and hospitals for long periods. Findings: An...

  • Acute Hemorrhagic Conjunctivitis Caused by Coxsackievirus A24 Variant, South Korea, 2002. Myoung-doh Oh; Sangwon Park; Yongju Choi; Hongbin Kim; Kiduk Lee; Wanbum Park; Youngae Yoo; Eui-Chong Kim; Kangwon Choe // Emerging Infectious Diseases;Aug2003, Vol. 9 Issue 8, p1010 

    Investigates the viral etiology of acute hemorrhagic conjunctivitis caused by coxsackievirus A24 in South Korea in 2002. Outbreak of acute hemorrhagic conjunctivitis in the country; Background of the study population; Nucleotide sequences of the protease 3c region.

  • coxsackievirus. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p201 

    An encyclopedia entry for "coxsackievirus" is presented. It refers to a group of viruses responsible for a range of diseases. It can be either type A or B. Hand, foot and mouth disease is the best known of the type A infections. It is a common childhood disease characterized by blistering of...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics