TITLE

Development of a new method for diagnosis of Group B Coxsackie genome by reverse transcription loop-mediated isothermal amplification

AUTHOR(S)
Jaianand, K.; Saravanan, N.; Gunasekaran, P.; Sheriff, A. K.
PUB. DATE
April 2011
SOURCE
Indian Journal of Medical Microbiology;Apr2011, Vol. 29 Issue 2, p110
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Coxsackie B viruses (genus, Enterovirus; family, Picornaviridae) can cause aseptic meningitis, encephalitis, pleurodynia, and fatal myocarditis, and are implicated in the pathogenesis of dilated cardiomyopathy. The differentiation of the group B Coxsackieviruses into their subtypes has potential clinical and epidemiological implications. Objective: In this study, we developed a one-step, single-tube genogroup-specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of group B Coxsackie genomes targeting 5′ UTR region. Materials and Methods: The amplification can be obtained in less than 1 hour by incubating all the reagents in a single tube with reverse transcriptase and Bst DNA polymerase at 63°C. Detection of gene amplification could be accomplished by agarose gel electrophoresis and the monitoring of gene amplifi cation can also be visualised with the naked eye by using SYBR green I fluorescent dye. Results: A total of 40 samples comprising 31 positive samples and 9 negative samples were used in this study for comparative evaluation. The results were compared with those from Real-Time Polymerase Chain Reaction (RT-PCR). None of the RT-PCR-positive samples were missed by RT-LAMP, thereby indicating a higher sensitivity of the RT-LAMP assay. Conclusion: Thus, due to easy operation without a requirement of sophisticated equipment and skilled personnel, the RT-LAMP assay reported here is extremely rapid, cost-effective, highly sensitive, and specifi c and has potential usefulness for rapid detection of non-polio enterovirus (NPEV) not only by well-equipped laboratories but also by peripheral diagnostic laboratories with limited fi nancial resources in developing countries.
ACCESSION #
61346944

 

Related Articles

  • Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control. Xing-Long Xiao; Ya-Qing He; Yi-Gang Yu; Hong Yang; Gu Chen; Hui-Fang Li; Jing-Wei Zhang; Dong-Mei Liu; Xiao-Feng Li; Xiao-Quan Yang; Hui Wu // Archives of Virology;Jan2009, Vol. 154 Issue 1, p121 

    The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71...

  • Rapid and sensitive identification of RNA from the emerging pathogen, coxsackievirus A6. Ojcius, David M.; Jie Yan; Lei Zhang; Xinying Wang; Yanjun Zhang; Liming Gong; Haiyan Mao; Cen Feng // Virology Journal;2012, Vol. 9 Issue 1, p1 

    Background: Hand, foot and mouth disease (HFMD) is caused by members of the family Picornaviridae in the genus Enterovirus. It has been reported that coxsackievirus A6 (CVA6) infections are emerging as a new and major cause of epidemic HFMD. Sporadic HFMD cases positive for CVA6 were detected in...

  • DEVELOPMENT OF A NEW METHOD FOR DIAGNOSIS OF COXSACKIE B5 VIRUSES BY REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION. Jaianand, K.; Gunasekaran, P.; Rajkumar, M.; Sheriff, A. K. // International Journal of Pharma & Bio Sciences;Jul-Sep2010, Vol. 1 Issue 3, p1 

    We developed a one-step, single-tube genogroup-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of group B Coxsackie genomes targeting 5' UTR region. The amplification can be obtained in less than 1 h by incubating all of the reagents in a...

  • coxsackievirus.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p538 

    An encyclopedia entry for "coxsackievirus," which refers to any of a group of viruses, the first of which was isolated in 1948 from two children in Coxsackie, New York, is presented.

  • Coxsackievirus B1 most commonly reported enterovirus serotype from 2007 to 2008. Blazek, Nicole; Haigh, Christen; Hasson, Matt; Ripa, Meredith // Infectious Diseases in Children;Oct2009, Vol. 22 Issue 10, p40 

    The article discusses research on the prevalence of coxsackievirus B1 in enterovirus infections in infants, by M. E. Wikswo, published in the 2009 issue of "Clinical Infectious Diseases."

  • Molecular characterization of coxsackievirus A21 in Shandong, China. Wang, Suting; Xu, Minglei; Lin, Xiaojuan; Liu, Yao; Xiong, Ping; Wang, Lijuan; Xu, Aiqiang; Tao, Zexin; Zhang, Dongfeng // Archives of Virology;Feb2016, Vol. 161 Issue 2, p437 

    Coxsackievirus A21 (CV-A21) is a rarely detected serotype belonging to the species Enterovirus C (EV-C). In this study, we report the isolation and genetic characterization of CV-A21 in Shandong Province, China, during 1997 to 2013. A total of 13 strains were obtained from surveillance of cases...

  • Rhombencephalitis and Coxsackievirus A16. Goto, Kazuna; Sanefuji, Masafumi; Kusuhara, Koichi; Nishimura, Yorihiro; Shimizu, Hiroyuki; Kira, Ryutaro; Torisu, Hiroyuki; Hara, Toshiro // Emerging Infectious Diseases;Oct2009, Vol. 15 Issue 10, p1689 

    A letter to the editor about coxsackievirus A16 and enterovirus 71 as causes of hand, foot and mouth disease (HFMD) is presented.

  • Human MLPA Probe Design (H-MAPD): a probe design tool for both electrophoresis-based and bead-coupled human multiplex ligation-dependent probe amplification assays. Jizu Zhi; Hatchwell, Eli // BMC Genomics;2008, Vol. 9, Special section p1 

    Background: Multiplex ligation-dependent probe amplification (MLPA) is an efficient and reliable technique for gene dosage analysis. Currently MLPA can be conducted on two platforms: traditional electrophoresis-based, and FlexMAP bead-coupled. Since its introduction in 2002, MLPA has been...

  • Coxsackievirus B3, Shandong Province, China, 1990-2010. Zexin Tao; Yanyan Song; Yan Li; Yao Liu; Ping Jiang; Xiaojuan Lin; Guifang Liu; Lizhi Song; Haiyan Wang; Aiqiang Xu // Emerging Infectious Diseases;Nov2012, Vol. 18 Issue 11, p1865 

    To determine the cause of a 2008 outbreak of aseptic meningitis in Shandong Province, China, we analyzed samples from outbreak patients and coxsackievirus B3 samples collected during 1990-2010 surveillance. The cause of the outbreak was coxsackievirus B3, genogroup D. Frequent travel might...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics