TITLE

N-glycan-defective breast cancer cells induce a phenotypic switch in polarization of bone marrow-derived macrophages

AUTHOR(S)
Haidan Chen; Huili Cai; Liang Chen; Xianglei Wu; Dongqing Li; Handan Chen
PUB. DATE
April 2011
SOURCE
Clinical & Investigative Medicine;Apr2011, Vol. 34 Issue 2, pE71
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To investigate the effect of N-glycan-defective mammary adenocarcinoma cells on the polarization of macrophages. Methods: N-glycan-defective breast cancer cells (MA782 cells) were prepared by swainsonine (SW) treatment and the cytotoxicity of SW to MA782 cells was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The Nglycan- defective MA782 cells were co-cultured with bone marrow-derived macrophages (BMDMs) for 48 h in vitro, and then the BMDMs and the co-cultured supernatant were analyzed for macrophage phenotypic using FQRT-PCR, FCM and ELISA. Results: SW-treated MA782 cells expressed defective N-glycan on the cell surface in a dose-dependent manner (*p<0.05). MTT assays showed that neither the 1 μg/mL nor 5μg/ mL SW treatments showed significant inhibition of MA782 cell growth in vitro. The expression of iNOS and agr-1 in the 5 μg/mL SW-treated group were 4.75-fold higher and 3.7-fold lower than that in the untreated group, respectively (*p<0.05). Mean fluorescence intensity of CD16/32 expressed in the cells treated with 5 μg/mL SW was significantly higher in comparison with the untreated group (65 vs. 7, *p<0.05), though the percentage of CD16/32-positive cells were not significantly different. Furthermore, the expression of CD206 and dectin-1 in the 5 μg/mL SW-treated group was significantly decreased (3.1±0.3% and 4.1±1.1%, respectively) in comparison with the untreated group (40±3% and 8.9±1.2%, respectively, both p<0.05). In addition, the 5 μg/mL SW-treated group secreted more TNF-alpha (350 ±25 pg/mL) and less IL-10 (89±7.2 pg/mL) than the untreated group (80 ±3 pg/mL and 150 ±10 pg/mL, respectively, both p<0.05). Conclusion: N-glycan-defective MA782 cells can induce the differentiation of BMDM into proinflammatory M1 macrophages in vitro.
ACCESSION #
61263465

 

Related Articles

  • Vitamin D kills breast cancer cells. Wishart, Ian // Investigate;Oct/Nov2012, Vol. 10 Issue 134, p28 

    The article presents research results revealing the potential of vitamin D solution in killing breast cancer cells in New Zealand. Scientists discover that the vitamin stimulates the immune system to control cancer. They note that it helps decrease the migration of breast cancer cells by 68%...

  • Green Tea Catechin, EGCG, Suppresses PCB 102-Induced Proliferation in Estrogen-Sensitive Breast Cancer Cells. Baker, Kimberly Mantzke; Bauer, Angela C. // International Journal of Breast Cancer;12/13/2015, p1 

    The persistence of polychlorinated biphenyls (PCBs) in the environment is of considerable concern since they accumulate in human breast tissue and may stimulate the growth of estrogen-sensitive tumors. Studies have shown that EGCG from green tea can modify estrogenic activity and thus may act as...

  • A High-Content, Multiplexed Screen in Human Breast Cancer Cells Identifies Profilin-1 Inducers with Anti-Migratory Activities. Joy, Marion E.; Vollmer, Laura L.; Hulkower, Keren; Stern, Andrew M.; Peterson, Cameron K.; Boltz, R. C. “Dutch”; Roy, Partha; Vogt, Andreas // PLoS ONE;Feb2014, Vol. 9 Issue 2, p1 

    Profilin-1 (Pfn-1) is a ubiquitously expressed actin-binding protein that is essential for normal cell proliferation and migration. In breast cancer and several other adenocarcinomas, Pfn-1 expression is downregulated when compared to normal tissues. Previous studies from our laboratory have...

  • Lycopene and other carotenoids inhibit estrogenic activity of 17β-estradiol and genistein in cancer cells. Keren Hirsch; Andrea Atzmon; Michael Danilenko; Joseph Levy; Yoav Sharoni // Breast Cancer Research & Treatment;Aug2007, Vol. 104 Issue 2, p221 

    Abstract  Epidemiological evidence suggests that carotenoids prevent several types of cancer, including mammary and endometrial cancers. On the other hand, such studies have also shown that estrogens are the most important risk factors for these cancer types. Genistein, the...

  • Potential Therapeutic Effect of Natural Killer Cells on Doxorubicin-Resistant Breast Cancer Cells In Vitro. Hwang, Mi-Hye; Li, Xiu Juan; Kim, Jung Eun; Jeong, Shin Young; Lee, Sang-Woo; Lee, Jaetae; Ahn, Byeong-Cheol // PLoS ONE;8/21/2015, Vol. 10 Issue 8, p1 

    Objective: The aim of this study was to explore the therapeutic effect of natural killer (NK) cells on human doxorubicin-sensitive and resistant breast adenocarcinoma. Methods: Human doxorubicin-sensitive and resistant breast cancer cell lines (MCF-7 and MCF-7/ADR) were tagged with renilla...

  • Anti-proliferative effect of Klimaktoplan on human breast cancer cells. Ahn, Ki; Yi, Kyung; Park, Hyun; Shin, Jung; Hur, Jun; Kim, Sun; Kim, Tak // Archives of Gynecology & Obstetrics;Oct2013, Vol. 288 Issue 4, p833 

    Objective: With the health concerns of menopausal hormone replacement therapy, alternatives have been sought. Klimaktoplan is a homeopathic formulation consisting of four main components and has been used for relief of menopausal symptoms for a long time. The study investigated the safety of...

  • Ulipristal acetate does not impact human normal breast tissue. Communal, Laudine; Vilasco, Myriam; Hugon-Rodin, Justine; Courtin, Aurélie; Mourra, Najat; Lahlou, Najiba; Dumont, Sylvie; Chaouat, Marc; Forgez, Patricia; Gompel, Anne // Human Reproduction;Sep2012, Vol. 27 Issue 9, p2785 

    BACKGROUND Antiprogestins are of growing interest for the development of new treatments in the gynecological field. Ulipristal acetate (UPA) is a progesterone receptor (PR) modulator considered for long-term administration in contraception and is currently being registered for the treatment of...

  • Transcription Factor Ets1 Cooperates with Estrogen Receptor α to Stimulate Estradiol-Dependent Growth in Breast Cancer Cells and Tumors. Kalet, Brian T.; Anglin, Sara R.; Handschy, Anne; O’Donoghue, Liza E.; Halsey, Charles; Chubb, Laura; Korch, Christopher; Duval, Dawn L. // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    The purpose of this study was to explore the role of transcription factor Ets1 in estrogen receptor α (ERα)-positive breast cancer progression. We expressed human Ets1 or empty vector in four human ERα-positive breast cancer cell lines and observed increased colony formation. Further...

  • New therapy options coming for advanced breast cancer. McCann, Jean // Drug Topics;2/5/2001, Vol. 145 Issue 3, p42 

    Reports on the testing of several therapy options for breast cancer. Subjects and methods used; Findings.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics