Identification and characterization of the donkey CSN1S2 I and II cDNAs

Cosenza, Gianfranco; Pauciullo, Alfredo; Annunziata, Anna Lidia; Rando, Andrea; Chianese, Lina; Marletta, Donata; Iannolino, Gabriella; Nicodemo, Davide; Berardino, Dino Di; Ramunno, Luigi
April 2010
Italian Journal of Animal Science;Apr2010, Vol. 9 Issue 2, p206
Academic Journal
The ás2 casein, encoded by the CSN1S2 gene, is one of the three Calcium sensitive caseins present in the milk of ruminants of zootechnical interest and in the milk of Equidae species (horse and donkey). In the present study, we cloned, sequenced and analysed two different donkey CSN1S2 cDNAs that we called CSN1S2 I and CSN1S2 II. The first, which spans over a fragment of 1016 nt, is constituted by 19 exons and encodes for a predicted protein (called ás2-I) of 221 aminoacids; the second, of which we determined the entire sequence (16 exons), encodes for a predicted peptide (called ás2-II) of 168 aminoacids. Alternative splicing and genetic markers are reported for both genes.


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