Endothelial cell adhesion and growth within a bioassay chamber using microstamped ECM proteins

Rubenstein, David A.; Frame, Mary D.
June 2011
JOM: The Journal of The Minerals, Metals & Materials Society (TM;Jun2011, Vol. 63 Issue 6, p84
Academic Journal
Our goal was to evaluate microvascular endothelial cell growth on microstamped patterns of extracellular matrix proteins (ECM). A combination of photo- and soft-lithography was used to make features ∼100 μm deep and 150μm wide. Polydimethylsiloxane imprints of features produced positive molds used to stamp collagen I, IV, laminin and fibronectin onto cleaned hydrophilic or hydrophobic glass coverslips. Human dermal microvascular endothelial cells were seeded at an initial density of 800 cells cm-2, and cultured for three days. Explanted murine aortas, serving as an initial source for autologous endothelial cells, were perfused at 240 μL min for 1 day. Cell morphology was also quantified on both the non-patterned glass and within the microstamped patterns. Viability was high (>90%) on all microstamped proteins, regardless of glass hydrophobicity. Viability was reduced on bare hydrophobic glass. Cell density was 4 or 8 fold higher on microstamped ECM proteins compared with hydrophilic or hydrophobic glass, respectively. Confluence was approached more rapidly on microstamped proteins. Thus, rapid concentrated growth of endothelial cells was markedly enhanced within microstamped ECM patterns on hydrophilic and hydrophobic glass.


Related Articles

  • Degradation of Fibronectin and Vitronectin and Vitronectin in Chronic Wound Fluid: Analysis by Cell Blotting, Immunoblotting, and Cell Adhesion Assays. Grinnell, Frederick; Chin-Han Ho; Wysocki, Annette // Journal of Investigative Dermatology;Apr92, Vol. 98 Issue 4, p410 

    We used a combination of cell blotting, immunoblotting, and cell adhesion assays to analyze fibronectin and vitronectin in wound fluid from acute and chronic wounds. Acute wound fluid (e.g., suction blister fluid, mastectomy fluid) contained intact fibronectin and vitronectin as major cell...

  • Multilayer coatings on biomaterials for control of MG-63 osteoblast adhesion and growth. Kirchhof, Kristin; Hristova, Kamelia; Krasteva, Natalia; Altankov, George; Groth, Thomas // Journal of Materials Science: Materials in Medicine;Apr2009, Vol. 20 Issue 4, p897 

    Here, the layer-by-layer technique (LbL) was used to modify glass as model biomaterial with multilayers of chitosan and heparin to control the interaction with MG-63 osteoblast-like cells. Different pH values during multilayer formation were applied to control their physico-chemical properties....

  • Integrin Receptors and RGD Sequences in Human Keratinocyte Migration: Unique Anti-Migratory: Unique Anti-Migratory Function of α3β1 Epiligrin Receptor. Kim, Janice P.; Zhang, Ken; Kramer, Randall H.; Schall, Thomas J.; Woodley, David T. // Journal of Investigative Dermatology;May92, Vol. 98 Issue 5, p764 

    The migration of keratinocytes over the wound bed plays an important role in the re-epithelialization of cutaneous wounds. However, the mechanisms by which keratinocytes migrate over extracellular matrix components are unknown. In this study, we sought to determine if the RGD sequences in matrix...

  • Adhesion and Function of Human Endothelial Cells Co-cultured on Smooth Muscle Cells. Charles Wallace; John Champion; George Truskey // Annals of Biomedical Engineering;Mar2007, Vol. 35 Issue 3, p375 

    Abstract??To evaluate interactions between human endothelial cells (ECs) and smooth muscle cells (SMCs) for the development of tissue-engineered vessels, we examined the adhesion and key cell properties of human ECs grown on quiescent human aortic SMCs. ECs attached to SMCs spread more slowly...

  • Comparative Study of Adhesion Molecule Expression in Nodular Lesions of Behçet Syndrome and Other Forms of Panniculitis. Cuyan Demirkesen; Nukhet Tüzüner; Mustafa Şenocak; İknur Türkmen; Hilal Aki; Nuray Kepil; Cem Mat; Hasan Yazici // American Journal of Clinical Pathology;Jul2008, Vol. 130 Issue 1, p28 

    Adhesion molecules have a role in many vasculitic disorders. Our aim was to evaluate the status of adhesion molecules in nodular lesions of Behçet syndrome (BS) and compare them with results for the 2 most common types of panniculitis, erythema nodosum (EN) and nodular vasculitis (NV). We...

  • Characterization of Expression and Modulation of Cell Adhesion Molecules on an Immortalized Human Dermal Microvascular Endothelial Cell Line (HMEC-1). Xu, Yuelin; Swerlick, Robert A.; Sepp, Norbert; Bosse, Diane; Ades, Edwin W.; Lawley, Thomas J. // Journal of Investigative Dermatology;Jun94, Vol. 102 Issue 6, p833 

    We have recently reported the creation of the first immortalized cell line derived from human dermal microvascular endothelial cells (HMEC-1). In preliminary studies this line was found to closely resemble microvascular endothelial cells in regard to many phenotypic characteristics. Because two...

  • Human Microvascular Endothelial Cell Seeding on Cr-DLC Thin Films for Heart Valve Applications. Ali, N.; Kousar, Y.; Gracio, J.; Titus, E.; Okpalugo, T. I.; Singh, V.; Pease, M.; Ogwu, A. A.; Meletis, E. I.; Ahmed, W.; Jackson, M. J. // Journal of Materials Engineering & Performance;Apr2006, Vol. 15 Issue 2, p7 

    In this investigation, Cr-modified diamond-like carbon (Cr-DLC) films were studied for potential applications in mechanical heart valves. Three Cr-DLC samples were prepared using a magnetron sputtering technique employing an intensified plasma-assisted processing (IPAP) system. The three samples...

  • Comments on the Clinical Application of Fibronectin in Dentistry. PEARSON, B. S.; KLEBE, R. J.; BOYAN, B. D.; MOSKOWICZ, D. // Journal of Dental Research;Feb1988, Vol. 67 Issue 2, p515 

    Several studies have demonstrated that citric acid demineralization of the root surface promotes tissue attachment. Since demineralization exposes collagen to which fibronectin binds, the role of fibronectin in the attachment of cells to the tooth surface has been of considerable interest. It is...

  • Extracellular Matrix Molecules Improve Periodontal Ligament Cell Adhesion to Anorganic Bone Matrix. Lallier, T. E.; Yukna, R.; Moses, R. L. // Journal of Dental Research;Aug2001, Vol. 80 Issue 8, p1748 

    Bone replacement graft (BRG) materials are used in periodontal therapy to encourage new bone formation. Extracellular matrix proteins may improve periodontal ligament fibroblast (PDLF) attachment to these materials. We demonstrate that PDLFs adhere well to the extracellular matrix (ECM) proteins...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics