TITLE

Effect of Gabapentin-Lactam and Gamma-Aminobutyric Acid/Lactam Analogs on Proliferation and Phenotype of Ovine Mesenchymal Stem Cells

AUTHOR(S)
Sauerbier, Sebastian; Gutwald, Ralf; Wiedmann-Al-Ahmad, Margit; Wolkewitz, Martin; Haberstroh, Jörg; Obermeyer, Julia; Kuenz, Arne; Betz, Helene; Wolter, Franziska; Duttenhöfer, Fabian; Schmelzeisen, Rainer; Nagursky, Heiner; Proksch, Susanne; Al-Ahmad, Ali
PUB. DATE
April 2011
SOURCE
Oral & Craniofacial Tissue Engineering;2011, Vol. 1 Issue 1, p20
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Classic tissue engineering consists of three components: scaffold, cells, and growth or differentiation factors. Currently, expensive bone morphogenetic proteins are the most common substance used for hard tissue regeneration. An alternative could be gamma-aminobutyric acid/lactam (GABAlactam) analogs. Materials and Methods: The effects of gabapentin-lactam, cis- and trans-8-tertbutyl-GABA-pentin-lactam (trans-TB-GBP-L), and phenyl-GABA-lactam were tested in this study on ovine mesenchymal stem cell (MSC) proliferation. MSCs were selected from bone marrow aspirate concentrate by plastic adherence and amplified. Aliquots of the cells were incubated in medium, with four different concentrations of the GABA-lactam analogs dissolved in dimethyl sulfoxide. Cells in medium with and without dimethyl sulfoxide served as controls. Cell proliferation was tested with a nonradioactive assay. Before and after GABA-lactam analog influence, the MSC character was evaluated by the ability of the cells to differentiate into osteoblasts, chondrocytes, and adipocytes. Results: Proliferation was significantly increased under the influence of the analogs, depending on their concentration. MSCs cultured in 1 nmol/L trans-TB-GBP-L showed the highest proliferation rate. The MSC character was not altered. Conclusions: GABA-lactam analogs could be suited to stimulate MSC proliferation for tissue engineering applications. Further in vivo studies are necessary to evaluate the possible clinical potential of GABA-lactam analogs for hard tissue regeneration.
ACCESSION #
61029752

 

Related Articles

  • Ecto-Mesenchymal Stem Cells from Facial Process: Potential for Muscle Regeneration. Nie, Xin; Xing, Yongjun; Deng, Manjin; Gang, Li; Liu, Rui; Zhang, Yongjie; Wen, Xiujie // Cell Biochemistry & Biophysics;Sep2014, Vol. 70 Issue 1, p615 

    Ecto-mesenchymal stem cells (EMSCs) originate from the cranial neural crest and participate in the formation of tooth, salivary, and muscle in early development stage. The transplantation of EMSCs, a potential source of myoblast stem cell, might improve muscle regeneration. The purpose of this...

  • Inductive effect of Deprenyl and Dimethyl sulfoxide on proliferation and survival of the mesenchymal stem cells. Taheri, F.; Kashani, M. Haji Ghasem; Ghorbanian, M. T.; Hosseinpour, L. // Journal of Gorgan University of Medical Sciences;Autumn2012, Vol. 14 Issue 3, p1 

    Background and Objective: Research have been focused on the applying the chemical inducer for trans-differentiation the adult BMSCs into neural cell. So that, at the first should investigate the toxcity effect of the chemical inducer on the induced cells. Plasticity and easy accessibility of...

  • Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells. Somaiah, Chinnapaka; Kumar, Atul; Mawrie, Darilang; Sharma, Amit; Patil, Suraj Dasharath; Bhattacharyya, Jina; Swaminathan, Rajaram; Jaganathan, Bithiah Grace // PLoS ONE;12/14/2015, Vol. 10 Issue 12, p1 

    Mesenchymal stem cells (MSC) can differentiate into several cell types and are desirable candidates for cell therapy and tissue engineering. However, due to poor cell survival, proliferation and differentiation in the patient, the therapy outcomes have not been satisfactory. Although several...

  • CURRENT EDITORIAL CHALLENGES: CRITERIA FOR SELECTING ARTICLES FOR PUBLICATION. Hengstler, Jan // EXCLI Journal;2019, Vol. 18, p1101 

    No abstract available.

  • Research advances of fibroblastic differentiation of bone marrow mesenchymal stem cells. FU Hong-hai; HE Yue // China Journal of Oral & Maxillofacial Surgery;May2011, Vol. 9 Issue 3, p248 

    Bone marrow derived mesenchymal stem cells (BMSCs) can differentiate into multiple cell lineages such as osteoblasts, chondrocytes, and adipocytes. Furthermore, it is relatively easily harvested. So, it has wide applications in tissue engineering and regenerative medicine. Under a specific...

  • Isolation, Characterization, and Differentiation of Stem Cells for Cartilage Regeneration. Beane, Olivia; Darling, Eric // Annals of Biomedical Engineering;Oct2012, Vol. 40 Issue 10, p2079 

    The goal of tissue engineering is to create a functional replacement for tissues damaged by injury or disease. In many cases, impaired tissues cannot provide viable cells, leading to the investigation of stem cells as a possible alternative. Cartilage, in particular, may benefit from the use of...

  • EFFICACY OF MESENCHYMAL STEM CELL INCORPORATION IN DIFFERENT COMPOSITE ELECTROSPUN COLLAGEN NANOFIBERS FOR DIABETIC WOUND HEALING. NITHYA, T. G. // Asian Journal of Pharmaceutical & Clinical Research;Apr-Jun2013, Vol. 6 Issue 2, p149 

    Currently available skin substitutes for wound healing often suffer from a range of problems including wound contraction, scar formation, and poor integration with host tissue. Engineering skin substitutes by tissue engineering approach has relied upon the creation of three-dimensional scaffolds...

  • Engineering cell attachments to scaffolds in cartilage tissue engineering. Steward, Andrew; Liu, Yongxing; Wagner, Diane // JOM: The Journal of The Minerals, Metals & Materials Society (TM;Apr2011, Vol. 63 Issue 4, p74 

    One of the challenges of tissue engineering, a promising cell-based treatment for damaged or diseased cartilage, is designing the scaffold that provides structure while the tissue regenerates. In addition to the scaffold material's biocompatibility, mechanical properties, and ease of...

  • A promising injectable scaffold: The biocompatibility and effect on osteogenic differentiation of mesenchymal stem cells. Ding, Ke; Zhang, Yu; Yang, Zhong; Xu, Jian // Biotechnology & Bioprocess Engineering;Feb2013, Vol. 18 Issue 1, p155 

    Chitosan/β-glycerophosphate/collagen (C/GP/Co) is a promising injectable scaffold in the bone tissue engineering. In this study, we prepared this scaffold and evaluated its biocompatibility and effects on the osteogenic differentiation of mesenchymal stem cells (MSCs). After fabrication, the...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics