TITLE

Optimization of culture conditions for mycoepoxydiene production by Phomopsis sp. Hant25

AUTHOR(S)
Thammajaruk, Narukjaporn; Sriubolmas, Nongluksna; Israngkul, Duangnate; Meevootisom, Vithaya; Wiyakrutta, Suthep
PUB. DATE
June 2011
SOURCE
Journal of Industrial Microbiology & Biotechnology;Jun2011, Vol. 38 Issue 6, p679
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Culture media and fermentation conditions for cultivation of an endophytic fungus Phomopsis sp. Hant25 were investigated in order to improve the yield of mycoepoxydiene, a novel fungal metabolite having potent cytotoxic activity against many cancer cell lines. Mycoepoxydiene accumulated in the culture broth during the stationary phase of fungal growth. Modified M1D medium was superior to malt Czapek, and Czapek yeast autolysate broths in supporting mycoepoxydiene production. Pellet growth was the morphological form that favored biosynthesis of mycoepoxydiene. This could be achieved by incubating the culture statically for 6 days before shaking at 120 rpm. Incorporation of a cellulose paper disc into the culture flask promoted fungal growth at the liquid surface, which accelerated mycoepoxydiene production and maximized the final yield to a level of 354 mg l, though fungal attachment to the solid support was not required. Since the peak concentration of mycoepoxydiene in the culture broth was followed by a steeply declining phase, the harvest time had to be precisely determined for maximum product yield. Understanding the factor(s) involved in rapid degradation of mycoepoxydiene could lead to improved final yields.
ACCESSION #
60727710

 

Related Articles

  • CYTOTOXICITY OF ISOLATED COMPOUNDS FROM THE EXTRACTS OF STRUCHIUM SPARGANOPHORA (LINN) KTZE ASTERACEAE. KASIM, LATEEF SAKA; FERRO, VALERIE; ODUKOYA, OLUWAKEMI A.; UKPO, GRACE EIGBIBHALU; SEIDEL, VERONIQUE; GRAY, ALEXANDER I.; WAIGH, ROGER // Pakistan Journal of Pharmaceutical Sciences;Oct2011, Vol. 24 Issue 4, p475 

    Chemical investigation of the leaves of Struchium sparganophora by the application of VLC, CL and PTLC resulted in isolation of three compounds. The cytotoxicity activity of these compounds on malignant human cultured cells was examined. Vernodalin showed a significant cytotoxic activity on the...

  • P1–160CONSTRUCTION OF A 3D CULTURE ASSAY SYSTEM FOR ANTI-CANCER DRUG TESTING. Terashima, M.; Sakai, K.; Fujita, Y.; De Velasco, M. A.; Nishio, K. // Annals of Oncology;Nov2013 Supplement 9, Vol. 24, pix77 

    No abstract available.

  • Cytotoxic metabolites from the cultures of endophytic fungi from Panax ginseng. Zheng, Cheng-Jian; Xu, Li-Li; Li, Yuan-Yuan; Han, Ting; Zhang, Qiao-Yan; Ming, Qian-Liang; Rahman, Khalid; Qin, Lu-Ping // Applied Microbiology & Biotechnology;Sep2013, Vol. 97 Issue 17, p7617 

    Two strains of endophytic fungi, Penicillium melinii Yuan-25 and Penicillium janthinellum Yuan-27, with strong anti- Pyricularia oryzae activity, were obtained from the roots of Panax ginseng. Based on bioactivity-oriented isolation, a new benzaldehyde derivative, ginsenocin ( 1), together with...

  • Novel cytotoxic agents: Epothilones. Goodin, Susan // American Journal of Health-System Pharmacy;5/15/2008 Supplement, Vol. 65 Issue S3, pS10 

    Purpose. The epothilones are effective antitumor medications for patients with breast cancer, including patients who have been previously treated with or are resistant to anthracyclines or the taxanes. Summary. With the best currently available therapies, the median survival time for patients...

  • Assessing Combinations of Cytotoxic Agents Using Leukemia Cell Lines. Reynolds, C. Patrick; Kang, Min H.; Keshelava, Nino; Maurer, Barry J. // Current Drug Targets;Jun2007, Vol. 8 Issue 6, p765 

    The mainstay of clinical anti-neoplastic chemotherapy is multi-agent combinations, most of which were developed empirically. To speed research and decrease costs, there is increasing interest in moving new drugs into clinical trials in potentially active combinations based upon pre-clinical...

  • The Cytotoxic Effect of PM 701 and its Fractions on Cell Proliferation of Breast Cancer Cells, McF7. Khorshid, Faten A. // World Academy of Science, Engineering & Technology;Mar2011, Issue 51, p454 

    No abstract available.

  • Cytotoxicity of Triphenyltin(IV) Methyl- and Ethylisopropyldithiocarbamate Compounds in Chronic Myelogenus Leukemia Cell Line (K-562). Awang, N.; Kamaludm, N. F.; Hamid, A.; Mokhtar, N. W. N.; Rajah, N. F. // Pakistan Journal of Biological Sciences;2012, Vol. 15 Issue 17, p833 

    Studies on the discovery of new cancer treatment by using metal-based compounds such as tin (Sn) has now greatly being synthesized and evaluated to identify their effectiveness and suitability to be developed as a new anticancer drug. Approach: This study was carried out to evaluate the...

  • IN VITRO AND IN VIVO ANTI-CANCER ACTIVITY OF LEAF EXTRACT OF BARLERIA GRANDIFLORA. NISHANT MANGLANI; SHILPA VAISHNAVA; DHAMODARAN, P.; HEMANT SAWARKAR // International Journal of Pharmacy & Pharmaceutical Sciences;2014, Vol. 6 Issue 3, p70 

    Objective: To evaluate anticancer activity of the Barleria grandiflora (Acanthacae) leaf on the different cancerous and normal cell lines such as A-549 (human lung cancer) cells, Dalton's lymphoma Ascites (DLA tumour cells) and Vero (African green monkey kidney) normal cells. Methods: In vitro...

  • Functional study of the 830C>G polymorphism of the human carboxylesterase 2 gene. Bellott, Ricardo; Le Morvan, Valérie; Charasson, Virginie; Laurand, Armelle; Colotte, Marthe; Zanger, Ulrich M.; Klein, Kathrin; Smith, Denis; Bonnet, Jacques; Robert, Jacques // Cancer Chemotherapy & Pharmacology;Mar2008, Vol. 61 Issue 3, p481 

    Carboxylesterase 2 (CES2) is involved in the activation of the anticancer drug irinotecan to its active metabolite SN-38. We previously identified a single nucleotide polymorphism (SNP), with an allele frequency around 10%, as possibly involved in enzyme expression (Clin Pharmacol Ther...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics