TITLE

Bioactive Coating of Titanium Surfaces with Recombinant Human β -Defensin- 2 (rHuβD2) May Prevent Bacterial Colonization in Orthopaedic Surgery

AUTHOR(S)
Pfeufer, Nadine Y.; Hofmann-Peiker, Karsten; Muhle, Mike; Warnke, Patrick H.; Weigel, Martin C.; Kleine, Michael
PUB. DATE
May 2011
SOURCE
Journal of Bone & Joint Surgery, American Volume;5/4/2011, Vol. 93-A Issue 9, p840
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: A promising strategy to prevent infections around orthopaedic titanium implants is to use naturally occurring cationic antimicrobial peptides (CAMPs) such as, the human β-defensin-2 as antibacterial coatings. Human antimicrobial peptides represent a part of the innate immune system and have a broad antimicrobial spectrum against bacteria, fungi, and viruses. Methods: In the present study, titanium surfaces were functionalized by four different self-assembled monolayers (SAM5) forming methoxy si lanes: (1) hexadecyltrimethoxysi lane; (2) dimethoxymethyloctylsi lane, (3) allyltrimethylsi lane, and (4) 3-aminopropyltrimethoxysilane. In addition, calf skin type-I collagen was cross-linked to the SAM surface 3-aminopropyltrimethoxysilane by means of two different treatments: (1) N-hydroxysuccinimide and (2)glutaraldehyde. The functionalized titanium surfaces were coated with recombinant human β-defensin-2 (rHuβD2), an antimicrobial peptide, and were tested for antibacterial activity against Escherichia co/i. The release of rHuβD2 was quantified by means of enzyme-linked immunosorbent assay (ELISA). Results: The coating of functionalized titanium surfaces with rHuβD2 was successful. Recombinant HuβD2 was eluted from the titanium surfaces continuously, yielding antimicrobial activity up to several hours. Antimicrobial activity with a killing rate of 100% was observed for all functionalized titanium surfaces after two hours of incubation. The dimethoxy- methyloctylsilane-functionalized titanium surface delivered 0.65 ig of rHuβD2 after six hours with a 60% bacterial killing rate. The silane-functionalized surfaces exhibited a faster release of antimicrobially active rHuβD2 compared with col- lagen modifications. Conclusions: Natural antibiotics such as rHuβD2 integrated into the metal surface of titanium implants may be a promising tool to prevent and control infections around orthopaedic implants.
ACCESSION #
60581121

 

Related Articles

  • Antimicrobial Approaches for Preventing Infections Associated with Surgical Implants. Darouiche, Rabih O. // Clinical Infectious Diseases;5/15/2003, Vol. 36 Issue 10, p1284 

    Because management of infections associated with surgical implants can be both difficult and costly, prevention of such infections remains a priority. Preventive strategies comprise systemic perioperative administration of antibiotics and local application of antimicrobial agents (antibiotics or...

  • Innovative antimicrobial coating of titanium implants with iodine. Tsuchiya, Hiroyuki; Shirai, Toshiharu; Nishida, Hideji; Murakami, Hideki; Kabata, Tamon; Yamamoto, Norio; Watanabe, Koji; Nakase, Junsuke // Journal of Orthopaedic Science;Sep2012, Vol. 17 Issue 5, p595 

    Background: Postoperative infection associated with implants remains a serious complication in orthopedic surgery. Several biomaterial surface treatments have been proposed as a means of reducing the incidence of implant-associated infections. In this study, a clinical trial was performed using...

  • Controversies in the Management of Open Fractures. O'Brien, C. L.; Menon, M.; Jomha, N. M. // Open Orthopaedics Journal;2014 Supplement, Vol. 8, p178 

    Open fractures are a common problem encountered by orthopaedic surgeons and comprise a broad spectrum of trauma. Management is guided by principle-based steps aimed at reducing the risk of gas gangrene or suppurative infections, whilst maintaining viability in a favourable soft tissue...

  • Antimicrobial Activity of TiO2 Nanoparticle-Coated Film for Potential Food Packaging Applications. Othman, Siti Hajar; Salam, Nurul Raudhah Abd; Zainal, Norhazlizam; Basha, Roseliza Kadir; Talib, Rosnita A. // International Journal of Photoenergy;2014, p1 

    Recent uses of titanium dioxide (TiO2) have involved various applications which include the food industry This study aims to develop TiO2 nanoparticle-coated film for potential food packaging applications due to the photocatalytic antimicrobial property of TiO2. The TiO2 nanoparticles with...

  • Prevention infection in orthopedic surgery implementation of the hemoprophylactic protocol. Osmanovic, Elvedin; Asceric, Mensura; Omerkic, Esed; Ljuca, Farid // HealthMed;2011, Vol. 5 Issue 5, p1308 

    Introduction: Antibiotic prophylaxis is defined as the use of antimicrobials in the absence of symptoms of infection, with the aim of preventing or reducing the incidence of infection after surgery. Aim: We analyzed the incidence of surgical wound infection in patients in whom a protection of...

  • Methodologies for assessing local surface texture features that are relevant to cell attachment. Forbes, Alistair; Tomlins, Paul; Gurdak, Elzbieta; Illsely, Matthew; James, Stuart; James, Elizabeth // Journal of Materials Science: Materials in Medicine;Aug2010, Vol. 21 Issue 8, p2463 

    In this paper, we describe techniques for extracting features from surface topography data, gathered by a 3D-microscopy system, on a length scale that is relevant for cell attachment. The feature parameters considered include standard surface roughness parameters applied to the complete surface...

  • Bioactive glass enhances bone ingrowth into the porous titanium coating on orthopaedic implants. Drnovšek, Nataša; Novak, Saša; Dragin, Urška; Čeh, Miran; Gorenšek, Matevž; Gradišar, Marko // International Orthopaedics;Aug2012, Vol. 36 Issue 8, p1739 

    Purpose: The aim of the study was to verify the ability of nanoparticulate bioactive glass (BAG) to infiltrate into the porous titanium (Ti) layer on Ti-based implants to promote osseointegration. Methods: The porous titanium layer on Ti-based implants was impregnated with nanoparticulate BAG....

  • Nanowire Ceramic Coating Boosts Titanium Implants. Smock, Doug // Design News;10/22/2007, Vol. 62 Issue 15, p50 

    The article reports that nanowire ceramics has the potential to enhance the life of titanium implants, according to study of researchers from the University of Arkansas. Commercialization of the technology is planned through coating of biocompatible titanium with ceramic nanowire material. It is...

  • Effect of biological implant surface coatings on bone formation, applying collagen, proteoglycans, glycosaminoglycans and growth factors. Stadlinger, Bernd; Pilling, Eckart; Mai, Ronald; Bierbaum, Susanne; Berhardt, Ricardo; Scharnweber, Dieter; Eckelt, Uwe // Journal of Materials Science: Materials in Medicine;Mar2008, Vol. 19 Issue 3, p1043 

    The aim of the present study was to evaluate six different implant surface coatings with respect to bone formation. Being major structural components of the extracellular matrix, collagen, the non-collagenous components decorin/chondroitin sulphate (CS) and the growth factors TGF-β1/BMP-4...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics