TITLE

Determination of phenolic compounds in medicinal herbs by reversed-phase HPLC

AUTHOR(S)
Temerdashev, Z. A.; Frolova, N. A.; Kolychev, I. A.
PUB. DATE
April 2011
SOURCE
Journal of Analytical Chemistry;Apr2011, Vol. 66 Issue 4, p407
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
n HPLC procedure is proposed for the determination of 12 phenolic compounds in plant tissues by reversed-phase HPLC with gradient elution and UV detection. The influence of pH, composition of the mobile phase, concentration of the organic modifier, and temperature on the separation of gallic, protocatechuic, trans-ferulic, trans-caffeic acid, rutin, quercetin, dihydroquercetin, and (_)-epicatechin for 30 min is studied. The lower limit of quantification of phenolic compounds is 1-2.5 μg/L. The procedure was applied to the determination of phenolic compounds in aqueous extracts of Hypericum perforatum; its sample was found to contain protocatechuic acid, (_)-epicatechin, and also rutin.
ACCESSION #
59871118

 

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