Dynamic filtering of 2D-IR correlation spectroscopy to determine thermal transitions in β-lactoglobulin

Richardson, Hugh H.; Sefara, Nelson L.; Magtoto, Noel P.; Caldwell, Meghan L.
March 2000
AIP Conference Proceedings;2000, Vol. 503 Issue 1, p173
Academic Journal
The temperature dependent spectra collected for a mixture of β-lactoglobulin A and B dissolved in phosphate buffer were analyzed using dynamic filtering of the generalized 2D-IR correlation algorithm. This methodology provided information along the temperature coordinate where the largest spectral variations occur. These signatures were used to locate unique state to state transitions at 50 °C, 65 °C, and 73 °C. © 2000 American Institute of Physics.


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