A study of the unfolding process of human serum albumin in the acidic pH region by two-dimensional infrared correlation spectroscopy

Murayama, Koichi; Czarnik-Matusewicz, Boguslawa; Wu, Yuqing; Tsenkova, Roumiana; Ozaki, Yukihiro
March 2000
AIP Conference Proceedings;2000, Vol. 503 Issue 1, p283
Academic Journal
The aim of this study was the investigation of the unfolding process of human serum albumin (HSA) at acidic pH by using two-dimensional infrared correlation spectroscopy. The synchronous correlation map showed amide bands corresponding to a helix structure of HSA. The asynchronous correlation pattern change occurred near pH 4. New cross peaks appeared in the asynchronous correlation map at pH 3.4. These results provide an evidence for the unfolding of HSA due to conformational changes. © 2000 American Institute of Physics.


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