Generalized 2D infrared spectroscopic study on protein structure of spider’s thread

Katayama, Norihisa; Miyazawa, Mitsuhiro
March 2000
AIP Conference Proceedings;2000, Vol. 503 Issue 1, p287
Academic Journal
Structural study on spider’s thread has been performed by infrared spectroscopy. Generalized 2D method was applied for analysis of conformational transition and denaturation processes by thermal perturbation. The frequency shifts and intensity changes of amide I and amide II bands indicate that the conformation change of this fibrous protein among helical, sheet, and unordered structures has taken place with the temperature increasing. The band intensity of amide A decreases above 230 °C, suggesting that the denaturation of the protein proceeds over this temperature. This paper also demonstrates that the Generalized 2D analysis is a powerful tool for band separation and assignment from featureless spectrum in amide I and II regions. © 2000 American Institute of Physics.


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