TITLE

The protective role of 5-HMF against hypoxic injury

AUTHOR(S)
Li, Ming-Ming; Wu, Li-Ying; Zhao, Tong; Xiong, Lei; Huang, Xin; Liu, Zhao-Hui; Fan, Xue-Lai; Xiao, Cheng-Rong; Gao, Yue; Ma, Yun-Bao; Chen, Ji-Jun; Zhu, Ling-Ling; Fan, Ming
PUB. DATE
September 2011
SOURCE
Cell Stress & Chaperones;Autumn2011, Vol. 16 Issue 3, p267
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In an attempt to find new types of anti-hypoxic agents from herbs, we identified 5-hydroxymethyl-2-furfural (5-HMF) as a natural agent that fulfills the criterion. 5-HMF, the final product of carbohydrate metabolism, has favorable biological effects such as anti-oxidant activity and inhibiting sickling of red blood cells. The role of 5-HMF in hypoxia, however, is not yet. Our pilot results showed that pretreatment with 5-HMF markedly increased both the survival time and the survival rate of mice under hypoxic stress. The present study was aimed to further investigate the protective role of 5-HMF and the underlying mechanisms in hypoxic injury using ECV304 cells as an in vitro model. ECV304 cells pretreated with or without 5-HMF for 1 h were exposed to hypoxic condition (0.3% O) for 24 h and then cell apoptosis, necrosis, the changes of mitochondrial membrane potential (MMP) and the expressions of phosphorylation- extracellular signal-regulated kinase (p-ERK) were investigated. Pretreatment with 5-HMF markedly attenuated hypoxia-induced cell necrosis and apoptosis at late stage ( p < 0.01). Furthermore, pretreatment with 5-HMF rescued both the decline of the MMP and the increase of p-ERK protein under hypoxia. In a word, these results indicated that 5-HMF had protective effects against hypoxic injury in ECV304 cells, and its effects on MMP and p-ERK may be involved in the mechanism.
ACCESSION #
59786729

 

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