Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus

Zhongzan Cao; Zongxi Han; Yuhao Shao; Heyuan Geng; Xiangang Kong; Shengwang Liu
January 2011
Proteome Science;2011, Vol. 9 Issue 1, p11
Academic Journal
Background: Avian infectious bronchitis (IB) is one of the most serious diseases of economic importance in chickens; it is caused by the avian infectious coronavirus (IBV). Information remains limited about the comparative protein expression profiles of chicken embryonic tissues in response to IBV infection in ovo. In this study, we analyzed the changes of protein expression in trachea and kidney tissues from chicken embryos, following IBV infection in ovo, using two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS). Results: 17 differentially expressed proteins from tracheal tissues and 19 differentially expressed proteins from kidney tissues were identified. These proteins mostly related to the cytoskeleton, binding of calcium ions, the stress response, anti-oxidative, and macromolecular metabolism. Some of these altered proteins were confirmed further at the mRNA level using real-time RT-PCR. Moreover, western blotting analysis further confirmed the changes of annexin A5 and HSPB1 during IBV infection. Conclusions: To the best of our knowledge, we have performed the first analysis of the proteomic changes in chicken embryonic trachea and kidney tissues during IBV infection in ovo. The data obtained should facilitate a better understanding of the pathogenesis of IBV infection.


Related Articles

  • Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection. Feng Cong; Xiaoli Liu; Zongxi Han; Yuhao Shao; Xiangang Kong; Shengwang Liu // BMC Genomics;2013, Vol. 14 Issue 1, p1 

    Background Infectious bronchitis virus (IBV), a prototype of the Coronaviridae family, is an economically important causative agent of infectious bronchitis in chickens and causes an acute and highly contagious upper respiratory tract infections that may lead to nephritis. However, the molecular...

  • Molecular Characterization of Major Structural Protein Genes of Avian Coronavirus Infectious Bronchitis Virus Isolates in Southern China. Mei-Lan Mo; Meng Li; Bai-Cheng Huang; Wen-Sheng Fan; Ping Wei; Tian-Chao Wei; Qiu-Ying Cheng; Zheng-Ji Wei; Ya-Hui Lang // Viruses (1999-4915);Dec2013, Vol. 5 Issue 12, p3007 

    To gain comprehensive genetic information of circulating avian coronavirus infectious bronchitis virus (IBV) isolates in China, analysis of the phylogenetic tree, entropy of the amino acid sequences, and the positive selection as well as computational recombinations of S1, M and N genes of 23...

  • Diverse gammacoronaviruses detected in wild birds from Madagascar. de Sales Lima, Francisco Esmaile; Gil, Patricia; Pedrono, Miguel; Minet, Cécile; Kwiatek, Olivier; Campos, Fabrício Souza; Spilki, Fernando Rosado; Roehe, Paulo Michel; Franco, Ana Cláudia; Maminiaina, Olivier Fridolin; Albina, Emmanuel; de Almeida, Renata Servan // European Journal of Wildlife Research;Aug2015, Vol. 61 Issue 4, p635 

    To date, infectious bronchitis virus (IBV) is potentially found in wild birds of different species. This work reports the survey of coronaviruses in wild birds from Madagascar based on the targeting of a conserved genome sequence among different groups of CoVs. Phylogenetic analyses revealed the...

  • The papain-like protease of avian infectious bronchitis virus has deubiquitinating activity. Yu, Liping; Zhang, Xiaorong; Wu, Tianqi; Wang, Yuyang; Meng, Jie; Liu, Qian; Niu, Xiaosai; Wu, Yantao // Archives of Virology;Jul2017, Vol. 162 Issue 7, p1943 

    Coronavirus papain-like proteases (PLPs) can act as proteases that process virus-encoded large replicase polyproteins and also as deubiquitinating (DUB) enzymes. Like the PLPs of other coronaviruses (CoVs), the avian infectious bronchitis virus (IBV) PLP catalyzes proteolysis of Gly-Gly...

  • Identification of a Major Xylanase from Aspergillus flavus as a 14-kD Protein. Mellon, Jay; Cotty, Peter; Callicott, Kenneth; Abbas, Hamed // Mycopathologia;Oct2011, Vol. 172 Issue 4, p299 

    Aspergillus flavus K49 secreted at least two xylanase activities when grown on a medium containing larch (wood) xylan as a sole carbon source. Enzyme activity was assayed using an agar medium containing Remazol Brilliant Blue R conjugated oat spelt xylan as substrate. Crude enzyme preparations...

  • Differential plasma proteome profiles of mild versus severe β-thalassemia/Hb E. Hatairaktham, Suneerat; Srisawat, Chatchawan; Siritanaratkul, Noppadol; Chiangjong, Wararat; Fucharoen, Suthat; Thongboonkerd, Visith; Kalpravidh, Ruchaneekorn // Annals of Hematology;Mar2013, Vol. 92 Issue 3, p365 

    The severity of thalassemia is currently classified based on clinical manifestations and multiple tests. In the present study, we performed a plasma proteome analysis to identify differentially expressed proteins compared between normal subjects and patients with mild and severe forms of...

  • Heat stress–induced response of the proteomes of leaves from Salvia splendens Vista and King. Hui Liu; Guozheng Shen; Xianping Fang; Qiaojuan Fu; Kangkang Huang; Yi Chen; Hong Yu; Yun Zhao; Le Zhang; Liang Jin; Songlin Ruan // Proteome Science;2013, Vol. 11 Issue 1, p1 

    Background: Salvia splendens Ker-Gawl, most commonly used in China to add a splash of brilliant color to the surroundings during the warm season, is subject to heat stress, which can greatly affect its growth and yield. Results: To gain a comprehensive understanding of heat-tolerance mechanisms...

  • Analysis of protein composition of rabbit aqueous humor following two different cataract surgery incision procedures using 2-DE and LC-MS/MS. Stastna, Miroslava; Behrens, Ashley; McDonnell, Peter J.; Van Eyk, Jennifer E. // Proteome Science;2011, Vol. 9 Issue 1, p8 

    Background: The aqueous humor (AH), a liquid of the anterior and posterior chamber of the eye, comprises many proteins with various roles and important biological functions. Many of these proteins have not been identified yet and their functions in AH are still unknown. Recently, our laboratory...

  • Phylogenetic analysis of the S1 glycoprotein gene of infectious bronchitis viruses isolated in China during 2009-2010. Luo, Hongbin; Qin, Jianping; Chen, Feng; Xie, Qingmei; Bi, Yingzuo; Cao, Yongchang; Xue, Chunyi // Virus Genes;Feb2012, Vol. 44 Issue 1, p19 

    As part of our ongoing surveillance program, 40 field strains of avian infectious bronchitis virus (IBV) were isolated from dead or diseased chicken flocks in different areas of China between 2009 and 2010. S1 glycoprotein genes of these strains were sequenced and analyzed with 38 strains...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics