Macromolecular composition of phloem exudate from white lupin (Lupinus albus L.)

Rodriguez-Medina, Caren; Atkins, Craig A.; Mann, Anthea J.; Jordan, Megan E.; Smith, Penelope M. C.
January 2011
BMC Plant Biology;2011, Vol. 11 Issue 1, p36
Academic Journal
Background: Members of the legume genus Lupinus exude phloem 'spontaneously' from incisions made to the vasculature. This feature was exploited to document macromolecules present in exudate of white lupin (Lupinus albus [L.] cv Kiev mutant), in particular to identify proteins and RNA molecules, including microRNA (miRNA). Results: Proteomic analysis tentatively identified 86 proteins from 130 spots collected from 2D gels analysed by partial amino acid sequence determination using MS/MS. Analysis of a cDNA library constructed from exudate identified 609 unique transcripts. Both proteins and transcripts were classified into functional groups. The largest group of proteins comprised those involved in metabolism (24%), followed by protein modification/turnover (9%), redox regulation (8%), cell structural components (6%), stress and defence response (6%) with fewer in other groups. More prominent proteins were cyclophilin, ubiquitin, a glycine-rich RNA-binding protein, a group of proteins that comprise a glutathione/ascorbate-based mechanism to scavenge oxygen radicals, enzymes of glycolysis and other metabolism including methionine and ethylene synthesis. Potential signalling macromolecules such as transcripts encoding proteins mediating calcium level and the Flowering locus T (FT) protein were also identified. From around 330 small RNA clones (18-25 nt) 12 were identified as probable miRNAs by homology with those from other species. miRNA composition of exudate varied with site of collection (e.g. upward versus downward translocation streams) and nutrition (e.g. phosphorus level). Conclusions: This is the first inventory of macromolecule composition of phloem exudate from a species in the Fabaceae, providing a basis to identify systemic signalling macromolecules with potential roles in regulating development, growth and stress response of legumes.


Related Articles

  • The future of lupin as a protein crop in Europe. Lucas, M. Mercedes; Stoddard, Frederick L.; Annicchiarico, Paolo; Frías, Juana; Martínez-Villaluenga, Cristina; Sussmann, Daniela; Duranti, Marcello; Seger, Alice; Zander, Peter M.; Pueyo, José J. // Frontiers in Plant Science;Sep2015, p1 

    Europe has become heavily dependent on soya bean imports, entailing trade agreements and quality standards that do not satisfy the European citizen's expectations. White, yellow, and narrow-leafed lupins are native European legumes that can become true alternatives to soya bean, given their...

  • Lupin (Lupinus albus) Protein Isolate (L-ISO) Has Adequate Nutritional Value and Reduces Large Intestinal Weight in Rats after Restricted and ad libitum Feeding. Caligari, Silvia; Chiesa, Giulia; Johnson, Stuart K.; Camisassi, Davide; Gilio, Donatella; Marchesi, Marta; Parolini, Cinzia; Rubio, Luis A.; Sirtori, Cesare R. // Annals of Nutrition & Metabolism;Nov2006, Vol. 50 Issue 6, p528 

    Background: A protein isolate from white lupin (Lupinus albus; L-ISO) has potential as a novel human food ingredient, but its nutritional effects are unknown. Methods: We evaluated protein quality and effects on body composition in rats of isoenergic diets of L-ISO, lactalbumin, or casein with...

  • DIVERSIDAD MORFOLÓGICA Y CONCENTRACIÓN DE PROTEÍNA DE LUPINUS SPP. EN LA REGIÓN CENTRO-ORIENTAL DEL ESTADO DE PUEBLA, MÉXICO. Lagunes-Espinoza, Luz Del Carmen; López-Upton, Javier; García-López, Eustolia; Jasso-Mata, Jesús; Delgado-Alvarado, Adriana; De Los Santos, Gabino García // Acta Botanica Mexicana;abr2012, Issue 99, p73 

    The present study aimed to characterize the diversity of Lupinus genus (Leguminosae) present in agricultural and forest ecosystems of eastern central region of the Mexican state of Puebla, and to estimate seed protein concentration in species identified. Natural populations were located from...

  • THE Wild ONE. Sawyer-Fay, Rebecca // Country Living;May2000, Vol. 23 Issue 5, p92 

    Provides information on wild lupines plants in the United States.

  • EFEITO DO TRATAMENTO TÉRMICO NA DIGESTIBILIDADE, SOLUBILIDADE E ÍNDICE DE ATIVIDADE DE UREASE EM TREMOÇO (LUPINUS ALBUS E LUPINUS ANGUSTIFOLIUS). Monteiro, Márcia Regina Pereira; Oliveira, Cíntia Tarabal; Silva, Lucianne Sangiorgi; Mendes, Fabrícia Queiroz; de Cássia Oliveira Sant'ana, Rita // Brazilian Journal of Food & Nutrition / Alimentos e Nutrição;Sep2010, Vol. 21 Issue 3, p487 

    Among the many studied legumes, the lupine is a source of vegetable protein with great potential for use in food. This legume species are indigenous to the Mediterranean and South America and most are grown Lupinus albus (white lupine) and Lupinus angustifolius (Blue Lupin). However, there are...

  • Introduction of novel legume crops in Serbia - White lupin (Lupinus albus). Mikić, Aleksandar; Mihailović, Vojislav; Ćupina, Branko; Đorđević, Vuk; Stoddard, Frederick L. // Field & Vegetable Crops Research / Ratarstvo i povrtarstvo;2010, Vol. 47 Issue 1, p21 

    The renewed interest in introducing white lupin in Serbia is its high crude protein content in grain dry matter of nearly 400 g kg-1, which makes it a potential supplement for soybean meal in animal feeding. The only collection of white and other lupins in Serbia is maintained at Institute of...

  • Identification of Potentially Cross-Reactive Peanut-Lupine Proteins by Computer-Assisted Search for Amino Acid Sequence Homology. Guarneri, Fabrizio; Guarneri, Claudio; Benvenga, Salvatore // International Archives of Allergy & Immunology;2005, Vol. 138 Issue 4, p273 

    Background: Allergic cross-reactions are an issue of major concern because of implications for public health. The molecular basis of cross-allergy is the similarity of epitopes belonging to proteins of different organisms. Lupine is an emerging cause of food allergy, which has become a...

  • White lupins now real contender as spring break crop. Abels, Charles // Farmers Weekly;1/16/2004, Vol. 140 Issue 3, p49 

    Provides information on the advantage of white lupins to farm growers in East Anglia, England in the spring of 2004. Hydrogen-ion concentration tolerance of its variety, Dieta; Premiums that can be expected from the crop; Seed cost.

  • Lupin Growing Area Set to Double. Swallow, Andrew // Farmers Weekly;3/21/2003, Vol. 138 Issue 11, p65 

    Reports on possible increase in farming of high protein crop called lupines in Great Britain. Reasons for the increase in lupine farming; Harvesting season for lupines; Prospects for higher yield of the crop.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics