TITLE

Note: An easy way to enable total internal reflection-fluorescence correlation spectroscopy (TIR-FCS) by combining commercial devices for FCS and TIR microscopy

AUTHOR(S)
Yordanov, Stoyan; Best, Andreas; Weisshart, Klaus; Koynov, Kaloian
PUB. DATE
March 2011
SOURCE
Review of Scientific Instruments;Mar2011, Vol. 82 Issue 3, p036105
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Total internal reflection-fluorescence correlation spectroscopy (TIR-FCS) is a powerful method for studying dynamic processes at liquid-solid interfaces that may have numerous applications in biology, physics, and material science. Despite of its power and versatility, however, the use of TIR-FCS is still rather limited. The main reason for this is the need of a complex, in-house constructed optical setup whose assembly and adjustment is a quite difficult task. Clearly, the availability of ready to use, commercial TIR-FCS setups will strongly boost the application of this important method in many research areas. In this note we show that although such setups are still not available in the market, a proper combination of commercial devices for confocal fluorescence correlation spectroscopy and for total internal reflection microscopy may enable TIR-FCS in a way that do not require any special optical alignments. Furthermore, we demonstrate the capabilities of the setup by measuring the diffusion coefficient of single dye molecule and quantum dots in the very proximity of a water-glass interface.
ACCESSION #
59744485

 

Related Articles

  • High pressure sample cell for total internal reflection fluorescence spectroscopy at pressures up to 2500 bar. Koo, Juny; Czeslik, Claus // Review of Scientific Instruments;Aug2012, Vol. 83 Issue 8, p085109 

    Total internal reflection fluorescence (TIRF) spectroscopy is a surface sensitive technique that is widely used to characterize the structure and dynamics of molecules at planar liquid-solid interfaces. In particular, biomolecular systems, such as protein adsorbates and lipid membranes can...

  • Hindered diffusion of colloidal particles very near to a wall: Revisited. Bevan, Michael A.; Prieve, Dennis C. // Journal of Chemical Physics;7/15/2000, Vol. 113 Issue 3 

    Total internal reflection microscopy is a technique for monitoring changes in the distance between a single microscopic sphere and a flat plate by measuring the intensity of light scattered by the sphere when illuminated by an evanescent wave. A histogram of scattering intensities can be used to...

  • The last few milliseconds in the life of a secretory granule: Docking, dynamics and fusion visualized by total internal reflection fluorescence microscopy (TIRFM). Oheim, M.; Loerke, Dinah; St├╝hmer, Walter; Chow, Robert H. // European Biophysics Journal;1998, Vol. 27 Issue 2, p83 

    Abstract We have monitored single vesicles (granules) in bovine adrenal chromaffin cells using an optical sectioning technique, total internal reflection fluorescence microscopy (TIRFM). With TIR, fluorescence excitation is limited to an optical slice near a glass/water interface. In cells...

  • Microscopy's Expanding Role. Karet, Gail; Studt, Tim // R&D Magazine;Aug2000, Vol. 42 Issue 8, p16 

    Discusses the impact of technology on the field of optical microscopy. Major technological changes in microscopy; Information on the optical technique called total internal reflection fluorescence microscopy; Recommendations for buying a microscope.

  • BLAZING FUTURE Paths in TIRF. Semiatin, Ilene K. // R&D Magazine;Aug2006, Vol. 48 Issue 8, p24 

    The article reports on the developments in total internal reflection fluorescence (TIRF) microscopy, a method for viewing single molecule and cellular fluorescence at locations where molecules interact with surfaces. A development has been the introduction of high-quality chromatic correction....

  • Detection of single-molecule DNA hybridization by using dual-color total internal reflection fluorescence microscopy. Kang, Seong Ho; Kim, Yun-Jeong; Yeung, Edward S. // Analytical & Bioanalytical Chemistry;Apr2007, Vol. 387 Issue 8, p2663 

    We examined the use of prism-type simultaneous dual-color total internal reflection fluorescence microscopy (TIRFM) to probe DNA molecules at the single-molecule level. The system allowed the direct detection of the complementary interactions between single-stranded probe DNA molecules (16-mer)...

  • Direct visualization of nanopatterns by single-molecule imaging. Erwen Mei; Sharonov, Alexey; Ferris, James H.; Hochstrasser, Robin M. // Applied Physics Letters;1/24/2005, Vol. 86 Issue 4, p043102 

    The structures of patterned surface regions were clearly visualized by superimposing a series of single-molecule images from a total internal reflection fluorescence microscope, clearly demonstrating that a single-molecule imaging method can be used to directly visualize nanostructures. The...

  • Si[sub 1-x]Ge[sub x]/Si asymmetric 2x2 electro-optical switch of total internal reflection type. Yong Gao; Xiding Liu // Applied Physics Letters;12/4/1995, Vol. 67 Issue 23, p3379 

    Examines the fabricated asymmetric two by two switches of total internal reflection type based on plasma dispersion. Growth of Si[sub 1-x]Ge[sub x] by molecular beam epitaxy; Optimum intersecting angle and crosstalk injection current; Presence of insertion loss and switch time.

  • Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana.  // Plant Methods;2011, Vol. 7 Issue 1, p27 

    The article cites a research study that uses a new technique known as variable-angle epifluorescence microscopy (VAEM) as tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, as plants have cell walls, thick peripheral layers surrounding the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics