Metallacarboranes for the labelling of DNA - Synthesis of oligonucleotides bearing a 3,3′-iron-1,2,1′,2′-dicarbollide complex

Olejniczak, Agnieszka B.
April 2011
Canadian Journal of Chemistry;Apr2011, Vol. 89 Issue 4, p465
Academic Journal
The present work reports the efficient synthesis of oligonucleotides bearing metallacarborane complexes containing iron (3,3′-iron-1,2,1′,2′-dicarbollide) on a solid support via the H-phosphonate method. This versatile methodology can be applied to the synthesis of DNA oligomers bearing various metallacarborane units. Dans ce travail, on rapporte la synthèse efficace d'oligonucléotides portant des métallacarboranes contenant du fer (3,3′-fer-1,2,1′2′-dicarbollide) sur un support solide en faisant appel à la méthode du H-phosphonate. Cette méthodologie versatile peut être appliquée à la synthèse d'oligomères de l'ADN portant diverses unités métallacarboranes.


Related Articles

  • Structural Properties of G,T-Parallel Duplexes. Aviñó, Anna; Cubero, Elena; Gargallo, Raimundo; González, Carlos; Orozco, Modesto; Eritja, Ramon // Journal of Nucleic Acids;2010, p1 

    The structure of G,T-parallel-stranded duplexes of DNA carrying similar amounts of adenine and guanine residues is studied by means of molecular dynamics (MD) simulations and UV- and CD spectroscopies. In addition the impact of the substitution of adenine by 8-aminoadenine and guanine by...

  • New Indocyanine Derivatives for the Synthesis of Fluorescently Labeled Oligonucleotides. Timofeev, Edward N.; Kuznetsova, Victoria E.; Zasedatelev, Alexander S.; Chudinov, Alexander V. // Letters in Organic Chemistry;Jan2009, Vol. 6 Issue 1, p71 

    New sulfonated Cy3 and Cy5 phosphoramidites and solid supports were synthesized. These building blocks were used in the synthesis of terminally or internally labeled oligodeoxynucleotides. Mild deprotection schemes were applied to the sulfocyanine-labeled oligonucleotides.

  • Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis. Cleary, Michele A.; Kilian, Kristopher; Yanqun Wang; Bradshaw, Jeff; Cavet, Guy; Wei Ge; Kulkarni, Amit; Paddison, Patrick J.; Chang, Kenneth; Sheth, Nihar; Leproust, Eric; Coffey, Ernest M.; Burchard, Julja; McCombie, W. Richard; Linsley, Peter; Hannon, Gregory J. // Nature Methods;Dec2004, Vol. 1 Issue 3, p241 

    Generation of complex libraries of defined nucleic acid sequences can greatly aid the functional analysis of protein and gene function. Previously, such studies relied either on individually synthesized oligonucleotides or on cellular nucleic acids as the starting material. As each method has...

  • Directed evolution as a tool for understanding and optimizing nucleic acid polymerase function. Brakmann, S. // Cellular & Molecular Life Sciences;Nov2005, Vol. 62 Issue 22, p2634 

    Polynucleotide polymerases play a crucial role in transmitting genetic information from generation to generation, and they are the most important reagents in biotechnology. Although classical crystal structure analyses as well as biochemical studies have significantly contributed to our...

  • RNA:DNA ratio for diagnosis of the nutritional condition of Japanese anchovy Engraulis japonicus larvae during the first-feeding stage. Kono, Naoaki; Tsukamoto, Youichi; Zenitani, Hiromu // Fisheries Science;Dec2003, Vol. 69 Issue 6, p1096 

    In order to establish criteria with which to evaluate the nutritional condition of wild first-feeding larvae of Japanese anchovy Engraulis japonicus, the whole body RNA:DNA ratio and daily protein growth rate of reared larvae were individually determined. Anchovy larvae were reared for up to 9...

  • Synthesis, Antibacterial and Antifungal Investigations of Fe (II) Complexes with Schiff Bases Derived from 2 - Hydroxy - 1 - Naphthaldehyde and Some Aliphatic Diamines. Aliyu, H. N.; Sani, U.; Galadima, A. // European Journal of Scientific Research;9/5/2011, Vol. 59 Issue 2, p276 

    Schiff base ligands derived from 2-hydroxy-1-naphthaldehyde and some aliphatic diamines were successfully employed to synthesize corresponding Fe(II) complexes. Both the ligands and the complexes were characterized by physicochemical, elemental and spectroscopic analyses. They were also...

  • Effects of nitrosyl complexes of iron with functional S-ligands on the activity of hydrolytic enzymes. Tat'yanenko, L.; Dobrokhotova, O.; Pikhteleva, I.; Poletaeva, D.; Kotel'nikov, A.; Rudneva, T.; Sanina, N.; Aldoshin, S. // Pharmaceutical Chemistry Journal;Feb2012, Vol. 45 Issue 11, p651 

    We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe(SR')(NO)] with R' = cysteamine and penicillamine - and of the neutral type - [Fe(SR?)(NO)] with R? = benzthiazoline - on the enzymatic activity of hydrolases - cyclic guanosine monophosphate phosphodiesterase...

  • Regioselectivity of substrate hydroxylation versus halogenation by a nonheme iron(IV)-oxo complex: possibility of rearrangement pathways. Quesne, Matthew; Visser, Sam // Journal of Biological Inorganic Chemistry;Aug2012, Vol. 17 Issue 6, p841 

    Several nonheme iron enzymes and biomimetic model complexes catalyze a substrate halogenation reaction. Recent computational studies (Borowski et al. J Am Chem Soc 132:12887-12898, ) on α-ketoglutarate dependent halogenase proposed an initial isomerization reaction that is important to give...

  • Heterogeneous Photodecolorization of Methyl Green Catalyzed by Fe(II)-o-Phenanthroline/Zeolite Y Nanocluster. Nezamzadeh-Ejhieh, Alireza; Shahriari, Elahe // International Journal of Photoenergy;2011, Special section p1 

    The potential of Fe(II)-orthophenatrolin, as doped with synthetic zeolite Y nanocluster (Na-Y) via complexation process, after wet impregnation of parent zeolite with FeSO4 aqueous solution, was studied as a photocatalyst in decolorization ofMethyl Green (MG) under UV irradiation. The...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics