Stem cell factor can be predictive for human IVF outcome

Salmassi, A.; Zorn, S.; Mettler, L.; Jonat, W.; Schmutzler, A. G.
June 2010
Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2010 S2 Supplement, Vol. 20, pS59
Academic Journal
Introduction: Granulocyte-colony-stimulating factor (G-CSF) is produced by haemopoietic cells and several non-haemopoietic cells, such as stromal cells, fibroblasts, granulosa cells and epithelial cells of the uterine endometrium. Materials and Methods: The level of SCF in serum and in FF was measured in 74 IVF/ICSI patients (group 1) on the day of follicular puncture (FP) by ELISA. In group 2 (n = 30) SCF levels were measured in serum in all cycle phases until gestation. Results: In group 1 there were no significant differences in the median SCF concentration between serum (797.12±162.1 pg/ml) and FF (788.74±161.1 pg/ml) on the day of oocyte retrieval. A significant and positive correlation was found between the levels of SCF related to the number of follicles (SCF/f) and total injected dose (D/f) (P <0.001, r = 0.85). The level of SCF/f in serum and FF in patients who did not become pregnant was significantly higher than in those who became pregnant (P <0.05). Patients with poor response showed the highest SCF/f level in serum (289.8±106 pg/ml) and a pregnancy rate of 11.1%. Patients with moderate response had lower SCF/f levels in serum (177.2±59 pg/ml) and a pregnancy rate of 31.5%. Patients with good response displayed the lowest SCF/f level in serum (78.8±33 pg/ml) and a pregnancy rate of 51.7% (c2, p = 0.001). In group 2 the SCF in serum increased gradually from stimulation day (st.) 6-8 through st. 9-11 and reached a peak on the day of hCG injection (P ≤ 0.04). On the day of oocyte retrieval the SCF levels dropped slightly but not significantly. On post retrieval days, from the day of FP through ET, implantation (FP +1w) to the day of confirmation of pregnancy (ET +2w), the SCF levels increased significantly and reached their highest level (P = 0.02). After implantation the SCF level decreased slightly and reached a plateau during gestation. Conclusions: Our data show that SCF is involved in follicle development and ovulation and plays an important role in the maintenance of pregnancy. It could also be a predictor of embryo implantation for IVF outcome.


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