Makoolati, Z.; Movahedin, M.; Forouzandeh-Moghadam, M.
October 2010
Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2010 S3 Supplemen, Vol. 20, pS70
Academic Journal
Introduction: Under appropriate culture conditions, stem cells can differentiate into germ cell lineage. The aim of this study was to evaluate the effect of bone morphogenetic protein 4 (BMP4) on the expression of Stra8, the germ cell-specific gene, in mouse embryonic stem cell (ESC) derived germ cells. Materials and Methods: Following the pluripotency confirmation of the CCE mouse ESC line by OCT-4 immunocytochemistry, XY cytogenetic verification was done through Sry gene expression. ESCs were cultured in order to 1-day-old EB formation (the first step of germ cell induction) and then cultured for 4 days in the presence or absence of 5 ng/ml BMP4 (the second step of germ cell induction). The expression of Stra8 was calculated in ESC, the first and the second steps of germ cell induction by quantitative RT-PCR. Data analysis was done with ANOVA and Tukey posttest. Results: The mean normalized expression of Stra8 was increased significantly in 1-day-old EB relative to ESCs. Also, Quantitative RT-PCR results showed that Stra8 was expressed in a higher significant rate in BMP4-treated cells compared to BMP4-free group, ESC and 1-day-old EB (P ≤ 0.05). Conclusion: The results confirmed that the EB system and the presence of BMP4 in a culture system with ESCs improve the differentiation of ESCs to germ cell.


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