Human Endometrial CD98 Is Essential for Blastocyst Adhesion

Domínguez, Francisco; Simón, Carlos; Quiñonero, Alicia; Ramírez, Miguel Ángel; González-Muñoz, Elena; Burghardt, Hans; Cervero, Ana; Martínez, Sebastián; Pellicer, Antonio; Palacín, Manuel; Sánchez-Madrid, Francisco; Yáñez-Mó, María
October 2010
PLoS ONE;2010, Vol. 5 Issue 10, p1
Academic Journal
Background: Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans. Methods and Principal Findings: Using two human endometrial cell lines characterized by low and high receptivity, we identified the membrane receptor CD98 as a novel molecule selectively and significantly associated with the receptive phenotype. In human endometrial samples, CD98 was the only molecule studied whose expression was restricted to the implantation window in human endometrial tissue. CD98 expression was restricted to the apical surface and included in tetraspanin-enriched microdomains of primary endometrial epithelial cells, as demonstrated by the biochemical association between CD98 and tetraspanin CD9. CD98 expression was induced in vitro by treatment of primary endometrial epithelial cells with human chorionic gonadotropin, 17-β-estradiol, LIF or EGF. Endometrial overexpression of CD98 or tetraspanin CD9 greatly enhanced mouse blastocyst adhesion, while their siRNA-mediated depletion reduced the blastocyst adhesion rate. Conclusions: These results indicate that CD98, a component of tetraspanin-enriched microdomains, appears to be an important determinant of human endometrial receptivity during the implantation window.


Related Articles

  • Role of Pinopodes. Kabir-Salmani, Maryam // Fertility Weekly;3/18/2013, p1 

    The article discusses a study conducted in Iran which explores the role of pinopodes, the markers of endometrial receptivity. It reveals that pinopodes could have a role in adhesion during early human embryo implantation, noting that the L-selectin ligand is present in the human pinopodes. It...

  • hCG Regulates Cytokines Produced by hEECs.  // Fertility Weekly;11/7/2011, p9 

    The article reports on a study from Australia that human chorionic gonadotrophin (hCG) helps endometrial receptivity during implantation by regulating cytokines produced by human endometrial epithelial cells (hEECs). The study shows a role for blastocyst and/or endometrial-derived hCG in...

  • Myeloid ecotropic viral integration site 1 (MEIS) 1 involvement in embryonic implantation. Bei Xu; Dirk Geerts; Kun Qian; Hanwang Zhang; Guijin Zhu // Human Reproduction;Jun2008, Vol. 23 Issue 6, p1394 

    BACKGROUND The HOXA10 homeobox gene controls embryonic uterine development and adult endometrial receptivity. The three-amino-acid loop extension (TALE) family homeobox genes like myeloid ecotropic viral integration site 1 (MEIS) provide enhanced target gene activation and specificity in...

  • The role of chorionic gonadotropin and Notch1 in implantation. Afshar, Yalda; Stanculescu, Adina; Miele, Lucio; Fazleabas, Asgerally T. // Journal of Assisted Reproduction & Genetics;Jul2007, Vol. 24 Issue 7, p296 

    Purpose Failed implantation is a major limiting factor in infertility and early pregnancy loss. In primates, human chorionic gonadotropin mediated inhibition of stromal cell apoptosis and their subsequent differentiation into decidual cells is critical for successful embryo implantation. A major...

  • What is the preferred method for timing natural cycle frozen--thawed embryo transfer? Weissman, Ariel; Levin, Dan; Ravhon, Amir; Eran, Horowitz; Golan, Avraham; Levran, David // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jul2009, Vol. 19 Issue 1, p66 

    Spontaneous ovulation during a natural menstrual cycle represents a simple and efficient method for synchronization between frozen embryos and the endometrium. The objective was to compare serial monitoring until documentation of ovulation, with human chorionic gonadotrophin (HCG) triggering,...

  • Rat epididymal epithelial cells and 17β-estradiol synthesis under hCG stimulation in vitro. Świder-Al-Amawi, Małgorzata; Marchlewicz, Mariola; Kolasa, Agnieszka; Wenda-Różewicka, Lidia; Wiszniewska, Barbara // Folia Histochemica et Cytobiologica;2007, Vol. 45 Issue 3, p255 

    Epithelial cells of human and animal epididymis display features of steroidogenic cells. Rat epididymal epithelial cells in vitro produce androgens which are converted to 17β-estradiol, and released into the medium. The regulation of the epididymal steroidogenesis is not fully understood but...

  • Molecular basis of implantation. Pellicer, A.; Dominguez, F.; Remohi, J.; Simón, C. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Nov2002 Supplement 1, Vol. 5 Issue S1, p44 

    The process of embryonic implantation needs coordinated development and communication between the blastocyst and the maternal endometrium. Considerable advances in the understanding of the cell biology of the human embryo and maternal endometrium have been achieved separately. However, the...

  • Effect of E2 Concentrations on the Day of hCG in GnRH Antagonist Cycles on Outcome.  // Fertility Weekly;2/8/2010, p6 

    The article presents a study which evaluates the effect of estradiol (E2) on the day of human chorionic gonadotrophin (hCG) administration and pregnancy outcomes by D. Kyrou in Belgium. The study "E2 concentrations on the day of hCG administration in GnRH antagonist cycles are not associated...

  • Transcriptome Analysis Reveals New Insights into the Modulation of Endometrial Stromal Cell Receptive Phenotype by Embryo-Derived Signals Interleukin-1 and Human Chorionic Gonadotropin: Possible Involvement in Early Embryo Implantation Bourdiec, Amélie; Calvo, Ezequiel; Rao, C. V.; Akoum, Ali // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    The presence of the conceptus in uterine cavity necessitates an elaborate network of interactions between the implanting embryo and a receptive endometrial tissue. We believe that embryo-derived signals play an important role in the remodeling and the extension of endometrial receptivity period....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics