The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana

Biao Jin; Li Wang; Jing Wang; Ke-Zhen Jiang; Yang Wang; Xiao-Xue Jiang; Cheng-Yang Ni; Yu-Long Wang; Nian-Jun Teng
January 2011
BMC Plant Biology;2011, Vol. 11 Issue 1, p35
Academic Journal
Background: The leaf is an important plant organ, and how it will respond to future global warming is a question that remains unanswered. The effects of experimental warming on leaf photosynthesis and respiration acclimation has been well studied so far, but relatively little information exists on the structural and biochemical responses to warming. However, such information is very important to better understand the plant responses to global warming. Therefore, we grew Arabidopsis thaliana at the three day/night temperatures of 23/18°C (ambient temperature), 25.5/20.5°C (elevated by 2.5°C) and 28/23°C (elevated by 5°C) to simulate the middle and the upper projected warming expected within the 21st century for this purpose. Results: The 28/23°C treatment significantly reduced the life span, total biomass and total weight of seeds compared with the other two temperatures. Among the three temperature regimes, the concentrations of starch, chlorophyll, and proline were the lowest at 28/23°C, whereas the total weight of seeds, concentrations of chlorophyll and proline, stomatal density (SD), stomatal conductance (gs), net CO2 assimilation rate (A) and transpiration rate (E) were the highest at 25.5/20.5°C. Furthermore, the number of chloroplasts per cell and mitochondrial size were highest at 25.5/20.5°C and lowest at 28/23°C. Conclusions: The conditions whereby the temperature was increased by 2.5°C were advantageous for Arabidopsis. However, a rise of 5°C produced negative effects, suggesting that lower levels of warming may benefit plants, especially those which belong to the same functional group as Arabidopsis, whereas higher levels of warming may produce negative affects. In addition, the increase in A under moderately warm conditions may be attributed to the increase in SD, chlorophyll content, and number of chloroplasts. Furthermore, starch accumulation in chloroplasts may be the main factor influencing chloroplast ultrastructure, and elevated temperature regulates plant respiration by probably affecting mitochondrial size. Finally, high SOD and CAT activities may enable plants grown at elevated temperatures to exhibit relatively high tolerance to temperature stress, thus alleviating the harmful effects of superoxide anion radicals and hydrogen peroxide.


Related Articles

  • Age-dependent changes in the functions and compositions of photosynthetic complexes in the thylakoid membranes of Arabidopsis thaliana. Nath, Krishna; Phee, Bong-Kwan; Jeong, Suyeong; Lee, Sun; Tateno, Yoshio; Allakhverdiev, Suleyman; Lee, Choon-Hwan; Nam, Hong // Photosynthesis Research;Nov2013, Vol. 117 Issue 1-3, p547 

    Photosynthetic complexes in the thylakoid membrane of plant leaves primarily function as energy-harvesting machinery during the growth period. However, leaves undergo developmental and functional transitions along aging and, at the senescence stage, these complexes become major sources for...

  • Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots. Sylak-Glassman, Emily; Zaks, Julia; Amarnath, Kapil; Leuenberger, Michelle; Fleming, Graham // Photosynthesis Research;Jan2016, Vol. 127 Issue 1, p69 

    We describe a technique to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We show how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with...

  • Test of an In Vivo Method to Detect Chloroplast Division in Crop Plants. Ping Zheng; Wettzel, Carolyn; Ammar, Karim; Girard, Anne-Marie Michelle; Rodermel, Steve; Thomas, David R.; Li Ning; Callis, James B.; Edwards, Gerry E.; Daley, Larry // Spectroscopy;Apr2002, Vol. 17 Issue 4, p16 

    Presents information on a study which described a novel spectrofluorometric method that allows for an in vivo observation of chloroplast division in leaves of Arabidopsis thaliana. Significance of chloroplasts; Occurrence of chloroplast division; Use of statistical analysis to test the...

  • Chloroplast NAD Kinase is Essential for Energy Transduction Through the Xanthophyll Cycle in Photosynthesis. Takahashi, Hideyuki; Watanabe, Ayako; Tanaka, Ayumi; Hashida, Shin-nosuke; Kawai-Yamada, Maki; Sonoike, Kintake; Uchimiya, Hirofumi // Plant & Cell Physiology;Dec2006, Vol. 47 Issue 12, p1678 

    Photosynthetic parameters of the nadk2 mutant of Arabidopsis thaliana, which is defective in chloroplast NAD kinase, were investigated. In this plant, the effective efficiency of photosynthetic electron transport (ΦII) and the quantum yield of open reaction centers of photosystem II...

  • A Survey of Chloroplast Protein Kinases and Phosphatases in Arabidopsis thaliana. Schliebner, I.; Pribil, M.; Zühlke, J.; Dietzmann, A.; Leister, D. // Current Genomics;May2008, Vol. 9 Issue 3, p184 

    Protein phosphorylation is a major mode of regulation of metabolism, gene expression and cell architecture. In chloroplasts, reversible phosphorylation of proteins is known to regulate a number of prominent processes, for instance photosynthesis, gene expression and starch metabolism. The...

  • Acclimation of Arabidopsis thaliana to the light environment: the relationship between photosynthetic function and chloroplast composition. Shaun Bailey; Peter Horton; Robin G. Walters // Planta;Mar2004, Vol. 218 Issue 5, p793 

    Plants respond to growth under different environmental conditions by adjusting the composition of the photosynthetic apparatus. To investigate the consequences of the acclimation strategies adopted by Arabidopsis thaliana, we have assessed the functioning of the photosynthetic apparatus in...

  • Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein. Alboresi, Alessandro; Dall'Osto, Luca; Aprile, Alessio; Carillo, Petronia; Roncaglia, Enrica; Cattivelli, Luigi; Bassi, Roberto // BMC Plant Biology;2011, Vol. 11 Issue 1, p62 

    Background: Reactive oxygen species (ROS) are unavoidable by-products of oxygenic photosynthesis, causing progressive oxidative damage and ultimately cell death. Despite their destructive activity they are also signalling molecules, priming the acclimatory response to stress stimuli. Results: To...

  • The short-term response of Arabidopsis thaliana (C3) and Zea mays (C4) chloroplasts to red and far red light. Zienkiewicz, Maksymilian; Drożak, Anna; Wasilewska, Wioleta; Bacławska, Ilona; Przedpełska-Wąsowicz, Ewa; Romanowska, Elżbieta // Planta;Dec2015, Vol. 242 Issue 6, p1479 

    Main conclusion: Light quality has various effects on photochemistry and protein phosphorylation in Zea mays and Arabidopsis thaliana thylakoids due to different degrees of light penetration across leaves and redox status in chloroplasts. The effect of the spectral quality of light (red, R and...

  • Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Qihua Ling; Weihua Huang; Jarvis, Paul // Photosynthesis Research;Feb2011, Vol. 107 Issue 2, p209 

    The SPAD-502 meter is a hand-held device that is widely used for the rapid, accurate and non-destructive measurement of leaf chlorophyll concentrations. It has been employed extensively in both research and agricultural applications, with a range of different plant species. However, its utility...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics