Spectral-domain phase microscopy with improved sensitivity using two-dimensional detector arrays

Singh, K.; Dion, C.; Lesk, M. R.; Ozaki, T.; Costantino, S.
February 2011
Review of Scientific Instruments;Feb2011, Vol. 82 Issue 2, p023706
Academic Journal
In this work we demonstrate the use of two-dimensional detectors to improve the signal-to-noise ratio (SNR) and sensitivity in spectral-domain phase microscopy for subnanometer accuracy measurements. We show that an increase in SNR can be obtained, from 82 dB to 105 dB, using 150 pixel lines of a low-cost CCD camera as compared to a single line, to compute an averaged axial scan. In optimal mechanical conditions, phase stability as small as 92 μrad, corresponding to 6 pm displacement accuracy, could be obtained. We also experimentally demonstrate the benefit of spatial-averaging in terms of the reduction of signal fading due to an axially moving sample. The applications of the improved system are illustrated by imaging live cells in culture.


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