Porównanie aktywności enzymatycznej grzybów Trichosporon i Geotrichum wyizolowanych z mastitis u krów

Krukowski, Henryk; Lisowski, Andrzej
December 2010
Medical Mycology / Mikologia;2010, Vol. 17 Issue 4, p233
Academic Journal
Introduction: Fungi of the genus Trichosporon and Geotrichum cause mastitis in cows, but are isolated very rarely, because the main etiologic agents of this disease are the yeast Candida Aim of study: The aim of this study was to compare the enzymatic activity of the yeast of the genus Trichosporon and Geotrichum isolated from 11 cases of mastitis in cows. Material and methods: The study included 6 strains of fungi of the genus Trichosporon and 5 strains of the genus Geotrichum isolated from cows with fungal inflammation of the udder. Enzymatic activity was determined using the API ZYM tests (bioMerieux) containing substrates for the detection of 19 hydrolases. Results: The greatest activity in both types of yeast showed leucine arylamidase, high activity of acid phosphatase, the activity of average: esterase, esterase lipase, valine and cystine arylamidase. The lowest activity was characterized by C14 lipase and N-acetyl-β-glukoseaminidase in Trichosporon, while in Geotrichum the two glucosidase. No activity showed α-fucosidase, α-mannosidase, β-glucuronidase, two galactosidase (α and β), α-chymotrypsine and tripsine. Conlusions: 1. The highest activity in both types of yeast showed leucine arylamidase and acid phosphatase. 2. α-fucosidase, α-mannosidase, β-glucuronidase, α- and β-galactosidase, α-chymotrypsin and trypsin did not show enzymatic activity in the examined fungi.


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