Porównanie wyników badań zanieczyszczenia powietrza grzybami pomieszczeń oddziału opieki długoterminowej z wykorzystaniem aparatów SAS SUPER 100 i AIR IDEAL

Krajewska-Kułak, Elżbieta; Łukaszuk, Cecylia; Gniadek, Agnieszka; Kraszyńska, Bogumiła; Macura, Anna B.; Kędziora-Kornatowska, Kornelia; Kornatowski, Tomasz
December 2010
Medical Mycology / Mikologia;2010, Vol. 17 Issue 4, p221
Academic Journal
Introduction: Monitoring of the microbiological environment in the healthcare facilities is particularly important, therefore, it is advisable to research improvement and modifying methods for air assessing. Aim of the study: To compare the results of fungal air pollution in the long-term care departments using SAS SUPER 100 and AIR IDEAL samplers. Materials and methods: The study material consisted of air taken from the long-term care department, corridors and outside the hospital building. Air samples were taken with samplers: SAS 100 (Merck) and AIR IDEAL (bioMerieux). Biological monitoring of surface contamination was performed using Count-Tact applicator. Identification of fungi was carried out in accordance with the applicable mycological procedures. Temperature and humidity were determined by thermohygrometer PWT-401 (Elmetron). Results: In the case of the sampler AIR IDEAL CFU/L air samples ranged from 350 to 850 and average of all measurements were 724.2±159.9, and the sampler SAS SUPER 100 - from 160 to 800 and average of all measurements were 455.3±250.73 (p=0.0016). The average number of colonies from the tested walls was 161.1±257.5 colonies of fungi, the average temperature – 23.5±0.90C, the average moisture – 51.2%±5.4, and average values of air flow – 0.04±0.002 m/s. From the air samples of both samplers was incubated 6 genera/species of fungi. Candia albicans fungi species and genus Penicillium species were most frequently isolated from SAS Super 100 sampler, and Penicillium species from AIR IDEAL sampler. From the samples taken from the walls three types of fungi – Candida albicans, non-Candida albicans and Penicillium species were isolated. Conclusions: In most studied rooms was shown statistically significant differences in the values of CFU/l, depending on the type of sampler. Analysis of the mycological culture of fungi obtained from air samples of SAS SUPER 100 IDEAL AIR samplers, allowed to state that it is not always isolated the same types and species of fungi. Postulate: It is advisable to conduct further studies of fungal air pollution, to see if the authors of the work on the problem, they can compare their using other measuring instruments.


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