BinaxNOW -- An immunochromatographic test for the diagnosis of human influenza viruses: Comparison with viral culture and polymerase chain reaction

Bhattacharya, S.; Osman, H.
January 2011
Indian Journal of Medical Microbiology;Jan2011, Vol. 29 Issue 1, p19
Academic Journal
Purpose: A rapid test for influenza viruses (Binax NOW®) was evaluated. Materials and Methods: In season-1, 35 respiratory samples were tested retrospectively; in season-2, 45 samples were tested prospectively [gold-standard: viral culture in season-1, culture+ reverse transcriptase-polymerase chain reaction (RT-PCR) in season-2]. Results: Sensitivity for Binax for influenza A was 59.3 and 0% in season-1 and -2, respectively. Sensitivity was low for influenza B (33.3% in season-1, 26.1% in season-2). Samples having low viral load were more likely to have a negative Binax test. Specificity of Binax was high (100 and 94.7% with influenza A and B, respectively). Conclusion: Sensitivity information provided in the kit insert does not always reflect post licensure performance in clinical settings.


Related Articles

  • Accuracy of rapid flu tests.  // Clinical Advisor;Apr2012, Vol. 15 Issue 4, p20 

    The article reports on the result of a study which shows that rapid influenza diagnostic tests (RDITs) were more sensitive for the detection of influenza A than for influenza B.

  • Effectiveness of Patient-Collected Swabs for Influenza Testing. Dhiman, Neelam; Miller, Rita M.; Finley, Janet L.; Sztajnkrycer, Matthew D.; Nestler, David M.; Boggust, Andy J.; Jenkins, Sarah M.; Smith, Thomas F.; Wilson, John W.; Cockerill III, Franklin R.; Pritt, Bobbi S. // Mayo Clinic Proceedings;Jun2012, Vol. 87 Issue 6, p548 

    Objective: To compare the effectiveness of self-collected and health care worker (HCW)-collected nasal swabs for detection of influenza viruses and determine the patients' preference for type of collection. Patients and Methods: We enrolled adult patients presenting with influenzalike illness to...

  • Lab-on-chip test can identify human strains of flu.  // Electronics Weekly;4/2/2008, Issue 2329, p5 

    The article presents information on In-Check, a lab-on-chip test for flu, released by STMicroelectronics NV. In-Check is stated to be a disposable silicon-based micromachined integrated polymerase chain reaction (PCR) reactor for amplifying DMA. The reactor is customisable by spotting its...

  • Respiratory Viral Coinfections Identified by a 10-Plex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay in Patients Hospitalized With Severe Acute Respiratory Illness—South Africa, 2009–2010. Pretorius, Marthi A; Madhi, Shabir A.; Cohen, Cheryl; Naidoo, Dhamari; Groome, Michelle; Moyes, Jocelyn; Buys, Amelia; Walaza, Sibongile; Dawood, Halima; Chhagan, Meera; Haffjee, Sumayya; Kahn, Kathleen; Puren, Adrian; Venter, Marietjie // Journal of Infectious Diseases;Dec2012 Supplement 1, Vol. 206 Issue suppl_1, pS159 

    Background. Data about respiratory coinfections with 2009 pandemic influenza A virus subtype H1N1 during the 2009–2010 influenza pandemic in Africa are limited. We used an existing surveillance program for severe acute respiratory illness to evaluate a new multiplex real-time polymerase...

  • Results From the First 30 Months of National Sentinel Surveillance for Influenza in Tanzania, 2008–2010. Mmbaga, V. M.; Mwasekaga, M. J.; Mmbuji, P.; Matonya, M.; Mwafulango, A.; Moshi, S.; Emukule, G.; Katz, M. A. // Journal of Infectious Diseases;Dec2012 Supplement 1, Vol. 206 Issue suppl_1, pS80 

    Limited data exist on the burden of influenza in developing countries. In 2008, in order to better understand the epidemiology of influenza virus infection in Tanzania, the Tanzanian Ministry of Health and Social Welfare created a sentinel surveillance system for influenza. At 5 hospitals across...

  • FEASIBILITY OF REAL TIME PCR OVER CELL CULTURE IN DIAGNOSIS OF INFLUENZA VIRUS INFECTION: AN EXPERIENCE OF GRADE I VIRAL DIAGNOSTIC LABORATORY OF DEVELOPING COUNTRY. Jain, Bhawana; Singh, Ajay K.; Dangi, Tanushree; Verma, Anil K.; Dwivedi, Mukesh; Mohan, Madan; Singh, K. P.; Jain, Amita // International Journal of Applied Biological Sciences;2014, Vol. 2 Issue 1, p97 

    Introduction: In spite of the discovery of viral culture technology about a century ago, its application in diagnostic labs is being used since 1970s. It served as the "gold standard" for virus detection for long. In recent years, rapid, technically less challenging, sensitive and highly...

  • Detection of Influenza A and B Viruses With the Sofia Analyzer. Lewandrowski, Kent; Tamerius, John; Menegus, Marilyn; Olivo, Paul D.; Lollar, Ron; Lee-Lewandrowski, Elizabeth // American Journal of Clinical Pathology;May2013, Vol. 139 Issue 5, p684 

    This report describes the clinical evaluation of a novel fluorescent immunoassay (FIA), Sofia Influenza A+B FIA (Quidel, San Diego, CA), for the rapid detection and differentiation of influenza A and B viruses. A total of 2,047 subjects provided nasal swabs and nasopharyngeal swabs or aspirates....

  • A Quantitative Assessment of the Efficacy of Surgical and N95 Masks to Filter Influenza Virus in Patients with Acute Influenza Infection. Johnson, D. F.; Druce, J. D.; Birch, C.; Grayson, M. L. // Clinical Infectious Diseases;7/15/2009, Vol. 49 Issue 2, p275 

    We assessed the in vivo efficacy of surgical and N95 (respirator) masks to filter reverse transcription-polymerase chain reaction (RT-PCR)-detectable virus when worn correctly by patients with laboratory-confirmed acute influenza. Of 26 patients with a clinical diagnosis of influenza, 19 had the...

  • Detection of pneumolysin and autolysin genes among antibiotic resistant Streptococcus pneumoniae in invasive infections. Sourav, S.; Patricia, A.; Sharma, S.; Kanungo, R.; Jayachandran, S.; Prashanth, K. // Indian Journal of Medical Microbiology;Jan2010, Vol. 28 Issue 1, p34 

    Purpose: To detect the presence of autolysin and pneumolysin genes among Streptococcus pneumoniae strains isolated from different disease entities among Indian patients. The study also attempted to determine antimicrobial susceptibility of the isolates. Materials and Methods: A total of 24 S....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics