Isolation and Characterization of Streptomyces sp. from Durg District of Chhattisgarh for Antimicrobial activity

Singh, N.; Rai, V.
January 2011
Current Trends in Biotechnology & Pharmacy;Jan2011, Vol. 5 Issue 1, p1043
Academic Journal
The present work was carried out in order to establish a suitable protocol for screening program for antimicrobial producing Streptomyces sp. from soil sample of Durg District of Chhattisgarh, INDIA. Fifty seven Streptomyces isolates with distinct characteristics were isolated and purified by serial dilution method on starch casein nitrate agar medium. These isolates were screened for their antimicrobial activity against twenty one test organisms obtained from the Microbial Type Culture Collection and Gene Bank (MTCC), IMTECH, Chandigarh. Morphological, physiological and biochemical characteristics indicated that these isolates belong to the genus Streptomyces and characterized as Streptomyces rimosus, Streptomyces chromogenus, Streptomyces rouchi-3, Streptomyces antibioticus-3, Streptomyces filipinensis, Streptomyces atroolivaceus, Streptomyces exfloliatus-2, Streptomyces halstedii, Streptomyces cyaneus, Streptomyces violaceus, Streptomyces oliovaceoviridis, Streptomyces lydicus, Streptomyces phaeochromogenes, Streptomyces chromofuscus . Among fifty seven isolates, nineteen exhibited antimicrobial activity against test pathogens, and out of these nineteen isolates, 11 isolates exhibited both antibacterial and antifungal activity while 8 exhibited only antibacterial activity.


Related Articles

  • Antimicrobial Properties of 'Kelulut' (Trigona Spp) Honey. Shahjahan, M.; Halim, Nurul A. Bt A.; N. J., Noor Izani // Malaysian Journal of Medical Sciences;Jan2007, Vol. 14 Issue 1, p107 

    INTRODUCTION The natural healing power of honey has been well documented. Most accounts on honey involve honey produced by the common honeybee (Apis spp.). Honey produced by stingless bee (Trigona spp.), commonly known as 'Kelulut' in Malaysia, is also known to have medicinal values. 'Kelulut'...

  • Chemical Composition and Antifungal Activity of the Fruit Oil of Zanthoxylum bungeanum Maxim. (Rutaceae) from China. Youwen Gong; Yongfu Huang; Ligang Zhou; Xueyan Shi; Zejian Guo; Mingan Wang; Weibo Jiang // Journal of Essential Oil Research;Mar/Apr2009, Vol. 21 Issue 2, p174 

    An essential oil obtained by water distillation from Zanthoxylum bungeanum Maxim. fruits was analyzed by GC and GC/MS. Terpinen-4-ol (19.7%), 1,8-cineole (16.0%), p-cymene (7.9%), γ-terpinene (7.3%) and α-terpineol (7.2%) were the major compounds of the 35 identified components. The...

  • Effect of different cultural, physiological and nutritional parameters on Streptomyces rochei (MTCC 10109) antimicrobial metabolites production. Reddy, N. Gopi; Ramakrishna, D. P. N.; Rajagopal, S. V. // Journal of Pharmacy Research;Aug2011, Vol. 4 Issue 8, p2675 

    In order to induce higher amount of antimicrobial metabolites production by Streptomyces rochei (10109) through fermentation, the cultural parameters has been studied in shake flasks. Antimicrobial metabolites production was started after 48 h of incubation and reached its optimum level at 10 %...

  • Isolating of microorganism resistance to crop pathogenic fungi and its polyketone synthase gene analysis. GUO Jia; WU Jinlong; TANG Yanping; TAN Hui; LAI Shujin; LI Yang; YANG Zhenfci; TAO Xinyuan; CHEN Xin; LI Xiaohua // Journal of Huazhong Normal University;Jun2014, Vol. 48 Issue 3, p399 

    ... R21 strain was isolated from the soil collected from Shenlongjia Hubei province with 5 pathogenic fungi of crop as indicator. ... R21 strain was classified as streptomyces by standard morphological, physiological properties and 16S rDNA alalysis. The experiments showed the antimicrobial...

  • Antifungal activities of actinomycete strains associated with high-altitude sagebrush rhizosphere. Jim�nez-Esquil�n, A. E.; Roane, T. M. // Journal of Industrial Microbiology & Biotechnology;Aug2005, Vol. 32 Issue 8, p378 

    The antifungal-producing potential of actinomycete populations from the rhizosphere of low-altitude sagebrush, Artemisia tridentata, has been examined. In a continued investigation of new sources of antifungal-producing microorganisms, this study examined the antifungal-producing potential of...

  • Purification and chemical characterization of antimicrobial compounds from a new soil isolate Streptomyces rimosus MTCC 10792. Singh, N.; Rai, V.; Tripathi, C. // Applied Biochemistry & Microbiology;Sep2013, Vol. 49 Issue 5, p473 

    A new isolate of Streptomyces sp. from soil of state Chhattisgarh (India) having broad spectrum antibacterial and antifungal activity was obtained. The active strain was identified as Streptomyces rimosus subsp. rimosus with accession number MTCC 10792 based on physiological, biochemical...

  • ANTIFUNGAL POTENTIAL OF PIGMENTED STREPTOMYCES KSRO4 FROM AGUMBE REGION OF WESTERN GHATS. Shobha, K. S.; Onkarappa, R.; Gautham, S. A. // Journal of Pharmaceutical & Scientific Innovation;Jan/Feb2014, Vol. 3 Issue 1, p43 

    A strain of Streptomyces isolated from agumbe soil of Western Ghats of Karnataka, India was studied with special references to the production of yellow colored pigment and the antibiotic properties of the same. The optimum temperature as 28C and the most favorable PH was 9. This strain used the...

  • Antifungal Chemical Compounds Identified Using a C. elegans Pathogenicity Assay. Breger, Julia; Fuchs, Beth Burgwyn; Aperis, George; Moy, Terence I.; Ausubel, Frederick M.; Mylonakis, Eleftherios // PLoS Pathogens;Feb2007, Vol. 3 Issue 2, p168 

    There is an urgent need for the development of new antifungal agents. A facile in vivo model that evaluates libraries of chemical compounds could solve some of the main obstacles in current antifungal discovery. We show that Candida albicans, as well as other Candida species, are ingested by...

  • Differential citral content of 15 lemongrass genotypes and their antimicrobial property. Kumar, Sanjay; Subudhi, Enketeswara; Nayak, Sanghamitra; Sahoo, Satyabrata // Internet Journal of Microbiology;Jan2009, Vol. 6 Issue 1, p4 

    In this present study antimicrobial activity of 15 promising cultivars of lemongrass were compared against 10 pathogenic microorganisms. Essential oil extracted from fresh leaves showed a distinctive variation in its oil percentage, citral content as well as antibacterial and antifungal...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics