TITLE

Rapid Quantification of Lipids in Acremonium chrysogenum Using Oil Red O

AUTHOR(S)
Hyun Yong Shin; Jin Young Lee; Eun Ji Kim; Seung Wook Kim
PUB. DATE
March 2011
SOURCE
Current Microbiology;Mar2011, Vol. 62 Issue 3, p1023
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml of lipids in A. chrysogenum.
ACCESSION #
58627823

 

Related Articles

  • Inclusion-induced boundary layers in lipid vesicles. Paolo Biscari; Gaetano Napoli // Biomechanics & Modeling in Mechanobiology;Sep2007, Vol. 6 Issue 5, p297 

    Abstract  The equilibrium shapes of lipid vesicles are perturbed by rigid inclusions. In a two-dimensional vesicle, that may also model a cylindrically elongated tubule, the shape modifications can be determined analytically, and turn out to be significant even far from the inclusion. On...

  • Dermatophyte Infections in Cairo, Egypt. S. Zaki; N. Ibrahim; K. Aoyama; Y. Shetaia; K. Abdel-Ghany; Y. Mikami // Mycopathologia;Mar2009, Vol. 167 Issue 3, p133 

    Abstract  In this study, we examined dermatophyte infections in patients referred to the Department of Dermatology, EL-Houd El-Marsoud Hospital, Cairo, during March 2004 to June 2005. Of 506 patients enrolled in this investigation, 403 (79.6%) were clinically diagnosed as having...

  • Keratinophilic fungi from selected soils of Bahrain. S. Deshmukh; Q. Mandeel; S. Verekar // Mycopathologia;Mar2008, Vol. 165 Issue 3, p143 

    Abstract  Eighty soil samples were collected from various sites of Bahrain and screened for presence of keratinophilic fungi using hair baiting techniques for isolation. Thirty-six isolates were recovered and identified. The cultures were identified using macro- and...

  • Nano- and Microdelivery Systems for Marine Bioactive Lipids. Pereira, David M.; Valentäo, Patricia; Andrade, Paula B. // Marine Drugs;2014, Vol. 12 Issue 12, p6014 

    There is an increasing body of evidence of the positive impact of several marine lipids on human health. These compounds, which include ω-3 polyunsaturated fatty acids, have been shown to improve blood lipid profiles and exert anti-inflammatory and cardioprotective effects. The high...

  • Biomimetic Silica Microspheres in Biosensing. Chemburu, Sireesha; Fenton, Kyle; Lopez, Gabriel P.; Zeineldin, Reema // Molecules;Mar2010, Vol. 15 Issue 3, p1932 

    Lipid vesicles spontaneously fuse and assemble into a lipid bilayer on planar or spherical silica surfaces and other substrates. The supported lipid bilayers (SLBs) maintain characteristics of biological membranes, and are thus considered to be biomembrane mimetic systems that are stable because...

  • Structure of apo-phosphatidylinositol transfer protein a provides insight into membrane association. Schouten, Arie; Agianian, Bogos; Westerman, Jan; Kroon, Jan; Wirtz, Karel W. A.; Gros, Piet // EMBO Journal;5/1/2002, Vol. 21 Issue 9, p2117 

    Phosphatidylinositol transfer protein a (PITPa) is a ubiquitous and highly conserved protein in multicellular eukaryotes that catalyzes the exchange of phospho-lipids between membranes in vitro and participates in cellular phospholipid metabolism, signal transduction and vesicular trafficking in...

  • Retrospective Comparison of the Effectiveness of a Fenofibrate 145 mg Formulation Compared with the Standard 160 mg Tablet. Davidson, Michael H.; Jones, Peter H. // Clinical Drug Investigation;2008, Vol. 28 Issue 10, p615 

    OBJECTIVE: To compare changes in lipid levels (total cholesterol [total-C], low-density lipoprotein cholesterol [LDL-C], triglycerides [TG], and high-density lipoprotein cholesterol [HDL-C]) for patients who switched from standard fenofibrate 160 mg (requiring dosing with food) to fenofibrate...

  • CHOLESTANOL IN AVIAN PLUMAGE. Bolliger, A.; Varga, D. // Australian Journal of Experimental Biology & Medical Science;Aug1960, Vol. 38 Issue 4, p265 

    Considerable amounts of cholestanol were consistently found in the feather lipids obtained by ether or chloroform extraction of the plumage of 14 (widely varying) species of birds including one ratite (emu). The cholestanol, which is the characteristic steroid of avian plumage, occurred mainly...

  • Cutaneous Blood Flow in Psoriasis. Klemp, Per; Staberg, Bent // Journal of Investigative Dermatology;Dec83, Vol. 81 Issue 6, p503 

    The disappearance rate of 133Xe was studied in 20 patients with psoriasis vulgaris, using an epicutaneous labeling technique in involved skin lesions or normal-appearing skin of the proximal extensor site of the forearm. Control experiments were performed in 10 normal subjects. Calculations of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics