Rapid Quantification of Lipids in Acremonium chrysogenum Using Oil Red O

Hyun Yong Shin; Jin Young Lee; Eun Ji Kim; Seung Wook Kim
March 2011
Current Microbiology;Mar2011, Vol. 62 Issue 3, p1023
Academic Journal
method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml of lipids in A. chrysogenum.


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