TITLE

CYTOTOXICITY EFFECT OF Curcuma aeruginosa EXTRACT ON FIBROBLAST WITH MTT ASSAY METHOD

AUTHOR(S)
Yuliawati, Tri Hartini; Hestianah, Eka Pramyrtha
PUB. DATE
April 2010
SOURCE
Folia Medica Indonesiana;abr2010, Vol. 46 Issue 2, p120
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The aim of this study was to find cytotoxicity effect of Curcuma aeruginosa extract on fibroblast with MTT assay method. This study used experimental laboratory with post test only control group design. Fibroblast cell lines culture divided into ten groups with four replication in each group. The positif control group was the group contained fibroblas cells in RPMI media. The negatif control group was the group contained media without cells. There were eight treatment groups given Curcuma aeruginosa extract in different concentration ( 1 ppm, 2 ppm, 2.5 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm). After exposure of the extract, the cells were incubated for twenty hours. The MTT reagen was given on the cells and then was incubated for four hours. After that the optic density was read with microplate reader in 630 wavelength. The result showed that the percentage of viable cells was more fifty percent in treatments groups. The Anova analysis showed that there was significantly decrease (p<0,05) of percentage of viable cell between positif control group and treatment groups. The conclusion was the Curcuma aeruginosa extract did not show cytotoxicity effect on fibroblast cells but the percentage of viable cell was decrease significantly in treatment groups.
ACCESSION #
58601612

 

Related Articles

  • In vitro screening of medicinal plant extracts for macrofilaricidal activity. Mathew Nisha; M. Kalyanasundaram; K. Paily; Abidha; P. Vanamail; K. Balaraman // Parasitology Research;Feb2007, Vol. 100 Issue 3, p575 

    Abstract??Methanolic extracts of 20 medicinal plants were screened at 1?10?mg/ml for in vitro macrofilaricidal activity by worm motility assay against adultSetaria digitata, the cattle filarial worm. Four plant extracts showed macrofilaricidal activity by worm motility at concentrations below...

  • IN VITRO BIOACTIVITIES OF CLOVE BUDS OIL (EUGENIA CARYOPHYLLATA) AND ITS EFFECT ON DERMAL FIBROBLAST. KHUNKITTI, WATCHAREE; VEERAPAN, PRAPHATSON; HAHNVAJANAWONG, CHARIYA // International Journal of Pharmacy & Pharmaceutical Sciences;Apr2012 Supplement 3, Vol. 4, p556 

    The aim of this study was to investigate the antioxidant and antityrosinase activities of clove oil and its components, the correlation between their antioxidant and antityrosinase activity and to examine the effect of clove bud oil towards dermal fibroblasts. The results show that the major...

  • Phytochemical Examination of Prosopis cineraria L. (Druce) Leaves. Malik, Archana; Kalidhar, S. B. // Indian Journal of Pharmaceutical Sciences;2007, Vol. 69 Issue 4, p576 

    The phytochemical studies on the leaves of Prosopis cineraria resulted in isolation of methyl docosanoate, diisopropyl-9,10-dihydroxyicosane-1,20-dioate, tricosan-1-ol and 7,24-tirucalladien-3-one. While diisopropyl-10,11-dihydroxyicosane-1,20-dioate is a hitherto unreported compound, methyl...

  • COMPARATIVE EVALUATION OF ANTIPYRETIC AND ANALGESIC ACTIVITIES OF RUNGIA REPENS NEES AND RUNGIA PECTINATA LINN. SWAIN, S. R.; SINHA, B. N.; MURTHY, P. N. // Asian Journal of Pharmaceutical & Clinical Research;Apr2011, Vol. 4 Issue 2, p103 

    The objective of the present study was to evaluate the anti-pyretic and analgesic activity of hydroalcoholic extract (50:50) of Rungia repens Nees and Rungia pectinata Linn. The anti-pyretic activity of both the plant was studied separately in Brewer's yeast-induced pyrexia in rats. The...

  • Caesalpinia gilliesii, Fabaceae.  // HerbalGram;May-Jul2015, Issue 106, p80 

    The article offers information about the ornamental plant, Caesalpinia gilliesii or bird of paradise. It cites that the species gilliesii was derived after Scottish botanist John Gillies, while the genus Caesalpinia is attributed to 16th century Italian botanist Andrea Cesalpino. It notes that...

  • Ethnobotanical Study OF Plants used to treat Diabetes by Tribal People of Kolli Hills, Namakkal District,Tamilnadu, Southern India. Elavarasi, S.; Saravanan, K. // International Journal of PharmTech Research;Jan-Mar2012, Vol. 4 Issue 1, p404 

    An Ethnobotanical study was conducted in the Kolli hills, Tamil Nadu through an oral interview to investigate the medicinal plants used in the treatment of Diabetes. In the present study, totally 16 species of plants belonging to 14 families were encountered. The present ethno-botanic study...

  • ISOLATION AND EVALUATION OF FENUGREEK, FLAXSEED MUCILAGES AND ITS USE AS A PHARMACEUTICAL BINDER. Inamdar, Mayur; Abhang, Pooja; Momin, Munira // International Journal of Pharmacy & Technology;Oct2012, Vol. 4 Issue 3, p4766 

    The objective of the present investigation was to evaluate the binder effects of mucilage isolated from flaxseed (Linum Usitatissimum L.) and fenugreek seed (Trigonella foenum graceum L.). Flax is an annual plant of the linaceae family and fenugreek is an annual herb of leguminosae family. These...

  • STUDY ON THE DIFFERENCES OF MAJOR PHARMACEUTICAL INGREDIENTS IN DIFFERENT PARTS AND PROCESSED MEDICINAL MATERIAL OF EPIMEDIUM BREVICORNU MAXIM IN TAIHANG MOUNTAIN. Jian Zai-you; Xu Gui-fang; Chen Hong-zhi; Wang Hong-sheng; Hu Xi-qiao; Zai-you, Jian; Gui-fang, Xu; Hong-zhi, Chen; Hong-sheng, Wang; Xi-qiao, Hu // Nutricion Hospitalaria;ago2015, Vol. 32 Issue 2, p913 

    The aim of this study is to evaluate the medicinal values of different parts of Epimedium brevicornu Maxim. and the effect of processing on major pharmaceutical ingredients in it. The contents of icariin and epimedin C in different parts and processed medicinal material of E. brevicornu in...

  • Portulaca oleracea L.: A Review of Phytochemistry and Pharmacological Effects. Zhou, Yan-Xi; Xin, Hai-Liang; Rahman, Khalid; Wang, Su-Juan; Peng, Cheng; Zhang, Hong // BioMed Research International;1/26/2015, Vol. 2015, p1 

    Portulaca oleracea L., belonging to the Portulacaceae family, is commonly known as purslane in English and Ma-Chi-Xian in Chinese. It is a warm-climate, herbaceous succulent annual plant with a cosmopolitan distribution. It is eaten extensively as a potherb and added in soups and salads around...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics